Polysorbate identity and quantity dictate the extensional flow properties of protein-excipient solutions

While protein medications are promising for treatment of cancer and autoimmune diseases, challenges persist in terms of development and injection stability of high-concentration formulations. Here, the extensional flow properties of protein-excipient solutions are examined via dripping-onto-substrate extensional rheology, using a model ovalbumin (OVA) protein and biocompatible excipients polysorbate 20 (PS20) and 80 (PS80). Despite similar PS structures, differences in extensional flow are observed based on PS identity in two regimes: at moderate total concentrations where surface tension differences drive changes in extensional flow behavior, and at small PS:OVA ratios, which impact the onset of weakly elastic flow behavior. Undesirable elasticity is observed in ultra-concentrated formulations, independent of PS identity; higher PS contents are required to observe these effects than in analogous polymeric excipient solutions. These studies reveal novel extensional flow behaviors in protein-excipient solutions, and provide a straightforward methodology for assessing the extensional flow stability of new protein-excipient formulations.     

Deployment of a cloud pipeline for real-time visual inspection using fast streaming high-definition images

We investigate the challenges of building an end-to-end cloud pipeline for real-time intelligent visual inspection system for use in automotive manufacturing. Current methods of visual detection in automotive assembly are highly labor intensive, and thus prone to errors. An automated process is sought that can operate within the real-time constraints of the assembly line and can reduce errors. Components of the cloud pipeline include capture of a large set of high-definition images from a camera setup at the assembly location, transfer and storage of the images as needed, execution of object detection, and notification to a human operator when a fault is detected. The end-to-end execution must complete within a fixed time frame before the next car arrives in the assembly line. In this article, we report the design, development, and experimental evaluation of the tradeoffs of performance, accuracy, and scalability for a cloud system.     

Using bald eagles to indicate the health of the Great Lakes' environment

The bald eagle (Haliaeetus leucocephalus) is one of the most studied birds of North America, and a great amount of natural life‐history information, including the response of various stressors on the eagles' ability to reproduce, are well known. In Michigan, the eagle has been chosen to track the trends of bioaccumulative compounds of concern across watersheds in the state. The state has been divided into major watersheds, and 20% of these are surveyed each year. A control area in northern Minnesota, Voyageurs National Park, is also sampled annually. We report here on the methods used, the preliminary results of the 1999 field season, and how differences in mercury concentrations varied over a 10‐year period. Mercury in feathers of nestling eagles declined over time only in Lakes Michigan and Huron, but have not decreased among other subpopulations in Michigan. Concentrations of polychlorinated biphenyls (PCBs) and 4,4′‐DDE in blood plasma from nestling eagles have declined over time for most subpopulations; however, they remain greater for breeding areas associated with the Great Lakes' food web. Sea eagles of the genus Haliaeetus are a good sentinel species to track trends in bioaccumulative compounds in aquatic systems.     

Learning about tools in infancy.

These experiments explored the role of prior experience in 12- to 18-month-old infants' tool-directed actions. In Experiment 1, infants' use of a familiar tool (spoon) to accomplish a novel task (turning on lights inside a box) was examined. Infants tended to grasp the spoon by its handle even when doing so made solving the task impossible (the bowl did not fit through the hole in the box, but the handle did) and even though the experimenter demonstrated a bowl-grasp. In contrast, infants used a novel tool flexibly and grasped both sides equally often. In Experiment 2, infants received training using the novel tool for a particular function; 3 groups of infants were trained to use the tool differently. Later, infants' performance was facilitated on tasks that required infants to grasp the part of the tool they were trained to grasp. The results suggest that (a) infants' prior experiences with tools are important to understanding subsequent tool use, and (b) rather than learning about tool function (e.g., hammering), infants learn about which part of the tool is meant to be held, at least early in their exposure to a novel tool. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Contracting, prompting, and reinforcing substance use disorder continuing care: A randomized clinical trial.

Although continuing care is strongly related to positive treatment outcomes for substance use disorder (SUD), participation rates are low and few effective interventions are available. In a randomized clinical trial with 150 participants (97% men), 75 graduates of a residential Veterans Affairs Medical Center SUD program who received an aftercare contract, attendance prompts, and reinforcers (CPR) were compared to 75 graduates who received standard treatment (STX). Among CPR participants, 55% completed at least 3 months of aftercare, compared to 36% in STX. Similarly, CPR participants remained in treatment longer than those in STX (5.5 vs. 4.4 months). Additionally, CPR participants were more likely to be abstinent compared to STX (57% vs. 37%) after 1 year. The CPR intervention offers a practical means to improve adherence among individuals in SUD treatment. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Bench-Scale Testing of Nanofiltration for Seawater Desalination

A dual-staged nanofiltration process is being evaluated as an alternative to reverse osmosis for seawater desalination. The primary goal of this system is to reduce energy consumption while producing potable water at an acceptable recovery rate. Investigation of this system at the bench-scale level focused on membrane surface characterization, ion rejection (including boron, bromide, and iodide rejection), and flux decline. Results from this study showed that two commercially available nanofiltration membranes can effectively desalinate seawater. Although fouling was apparent—and resulted in approximately 20% flux decline over 3 days—a critical flux was not identifiable. Operation of the system at different cross-flow velocities revealed the significance of hydrodynamic conditions on the polarization modulus, and hence on membrane performance.     

Stream Classification System Based on Susceptibility to Algal Growth in Response to Nutrients

We developed a stream classification system that is based on stream’s susceptibility to algal growth using a two-step approach. The model portrays algal biomass as a result of stream’s response to nutrient concentrations and the response is governed by various stream factors. In the first step, a nutrient-chlorophyll a relationship was developed to characterize nutrient’s effects on algal biomass. Residuals of the relationship were attributed to stream’s susceptibility to algal growth in response to nutrients and referred to as “observed” susceptibility. In the second step, conditions of other contributing factors were used to explain the variation in the residuals and the developed relationship was used to generate “predicted” susceptibility. Existing data compiled from various monitoring projects of Illinois streams and rivers were used to illustrate the approach. Streams were classified into three (high, medium, and low) categories based on their observed and predicted susceptibility values, respectively. With the available data, the model showed a 40-50% success rate for classifying the streams based on three observed and predicted susceptibility categories. Model entropy also was calculated for selecting the best model. The results show the important role of both nutrients and other contributing factors in explaining the variation of algal biomass. The study also suggests ways to fine tune the model and improve its accuracy, which would make the presented model a more viable tool for stream classification for establishing nutrient criteria to prevent surface streams from eutrophication.     

Use of modelling for optimization and upgrade of a tropical wastewater treatment plant in a developing country.

This paper presents results of a novel application of coupling the Activated Sludge Model No. 3 (ASM3) and the Anaerobic Digestion Model No.1 (ADM1) to assess a tropical wastewater treatment plant in a developing country (Surat, India). In general, the coupled model was very capable of predicting current plant operation. The model proved to be a useful tool in investigating various scenarios for optimising treatment performance under present conditions and examination of upgrade options to meet stricter and upcoming effluent discharge criteria regarding N removal. It appears that use of plant-wide modelling of wastewater treatment plants is a promising approach towards addressing often complex interactions within the plant itself. It can also create an enabling environment for the implementations of the novel side processes for treatment of nutrient-rich, side-streams (reject water) from sludge treatment.     

TI:无线通信创新无限

9月25日,正值中国传统节日中秋佳节,德州仪器(TI)无线通信事业部门的高层领导及其合作伙伴在"无线通信创新无限"-2007德州仪器无线高峰论坛上汇聚一堂,对无线通信技术的创新与应用,未来无线通信市场的机遇与挑战进行了深度沟通与探讨.论坛现场还实机演示了TI各项最新的手机技术应用,气氛融洽愉快.