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排序方式: 共有123条查询结果,搜索用时 31 毫秒
1.
Ciro Vasmara Massimo Pindo Diego Micheletti Rosa Marchetti 《International Journal of Hydrogen Energy》2018,43(18):8707-8717
This study addresses for the first time the influence of initial pH on the evolution of microbial consortia in dark fermentation of scotta permeate, using a high-throughput sequencing approach. Three fermentation phases could be detected: 1) a lag phase with no substantial differences in microbial composition at different initial pH values; 2) an exponential H2 production phase, accompanied by a general increase of Clostridium genus components and higher incidence of Trichococcus genus at neutral and alkaline pH; 3) a final stationary phase, characterized by a general increase of Bifidobacterium and Lactobacillus genera in all reactors. The initial pH value influenced the relative abundance of Trichococcus at 16–48 h of incubation. The metabolic activity of this genus increased the amount of metabolic precursors of H2 so that, when pH lowered to 5.4, clostridia in the reactors with initial alkaline pH become more active H2-producers than those in the others. 相似文献
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Vázquez Elisa Gomar Jéssica Ciurana Joaquim Rodríguez Ciro A. 《The International Journal of Advanced Manufacturing Technology》2015,81(1-4):219-229
The International Journal of Advanced Manufacturing Technology - The demand for micro holes, micro-molds, and micro forms continues to grow as high-tech industries demand miniaturized products.... 相似文献
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Sahana Gopal Ciro Chiappini Jelle Penders Vincent Leonardo Hyejeong Seong Stephen Rothery Yuri Korchev Andrew Shevchuk Molly M. Stevens 《Advanced materials (Deerfield Beach, Fla.)》2019,31(12)
Owing to their ability to efficiently deliver biological cargo and sense the intracellular milieu, vertical arrays of high aspect ratio nanostructures, known as nanoneedles, are being developed as minimally invasive tools for cell manipulation. However, little is known of the mechanisms of cargo transfer across the cell membrane‐nanoneedle interface. In particular, the contributions of membrane piercing, modulation of membrane permeability and endocytosis to cargo transfer remain largely unexplored. Here, combining state‐of‐the‐art electron and scanning ion conductance microscopy with molecular biology techniques, it is shown that porous silicon nanoneedle arrays concurrently stimulate independent endocytic pathways which contribute to enhanced biomolecule delivery into human mesenchymal stem cells. Electron microscopy of the cell membrane at nanoneedle sites shows an intact lipid bilayer, accompanied by an accumulation of clathrin‐coated pits and caveolae. Nanoneedles enhance the internalization of biomolecular markers of endocytosis, highlighting the concurrent activation of caveolae‐ and clathrin‐mediated endocytosis, alongside macropinocytosis. These events contribute to the nanoneedle‐mediated delivery (nanoinjection) of nucleic acids into human stem cells, which distribute across the cytosol and the endolysosomal system. This data extends the understanding of how nanoneedles modulate biological processes to mediate interaction with the intracellular space, providing indications for the rational design of improved cell‐manipulation technologies. 相似文献
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We studied the influence of initial pH on hydrogen (H2) production using permeate from scotta (a partially deproteinized cheese whey from ricotta production) as substrate (51 g L?1 lactose). Dark fermentation was carried out at 35 °C in laboratory batch reactors, in an unbuffered system. Hydrogen production and metabolite (volatile fatty acids, ethanol, and lactic acid) evolution during a 96-h period were monitored in reactors with initial pH varying in the range 4–10. In all reactors, H2 production started only when pH fell below 6. However, it was much higher (+31%) in the reactors with initial alkaline pH. We conclude that H2 production occurs only at acidic pH values, but initial alkaline pH values increase the overall H2 production in dark fermentation of lactose-rich substrates. 相似文献
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We relate indicators of cognitive abilities and health promotion to the propensity to screen for breast cancer, using microeconomic data available in a sample of women aged 50 or above in 11 European countries. Our findings suggest that health promotion activities attenuate the effect of human capital on the decision to undertake mammography, thus implying that disparities in education achievements and cognitive skills do not fully translate into differences in the propensity to do preventive screening. 相似文献
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