全文获取类型
收费全文 | 1448篇 |
免费 | 171篇 |
国内免费 | 4篇 |
专业分类
电工技术 | 15篇 |
综合类 | 2篇 |
化学工业 | 387篇 |
金属工艺 | 15篇 |
机械仪表 | 39篇 |
建筑科学 | 58篇 |
矿业工程 | 2篇 |
能源动力 | 68篇 |
轻工业 | 269篇 |
水利工程 | 20篇 |
石油天然气 | 6篇 |
无线电 | 95篇 |
一般工业技术 | 212篇 |
冶金工业 | 73篇 |
原子能技术 | 4篇 |
自动化技术 | 358篇 |
出版年
2024年 | 4篇 |
2023年 | 25篇 |
2022年 | 23篇 |
2021年 | 92篇 |
2020年 | 62篇 |
2019年 | 78篇 |
2018年 | 87篇 |
2017年 | 87篇 |
2016年 | 108篇 |
2015年 | 64篇 |
2014年 | 88篇 |
2013年 | 138篇 |
2012年 | 107篇 |
2011年 | 142篇 |
2010年 | 73篇 |
2009年 | 71篇 |
2008年 | 74篇 |
2007年 | 62篇 |
2006年 | 54篇 |
2005年 | 34篇 |
2004年 | 25篇 |
2003年 | 20篇 |
2002年 | 28篇 |
2001年 | 8篇 |
2000年 | 7篇 |
1999年 | 10篇 |
1998年 | 12篇 |
1997年 | 10篇 |
1996年 | 11篇 |
1995年 | 3篇 |
1994年 | 2篇 |
1993年 | 3篇 |
1990年 | 1篇 |
1988年 | 2篇 |
1987年 | 2篇 |
1985年 | 3篇 |
1981年 | 2篇 |
1978年 | 1篇 |
排序方式: 共有1623条查询结果,搜索用时 15 毫秒
1.
Laura Cuy-Chaparro Michel David Bohrquez Gabriela Arvalo-Pinzn Jeimmy Johana Castaeda-Ramírez Carlos Fernando Surez Laura Pabn Diego Ordez Gina Marcela Gallego-Lpez Carlos Esteban Surez Darwin Andrs Moreno-Prez Manuel Alfonso Patarroyo 《International journal of molecular sciences》2021,22(2)
Apical membrane antigen 1 is a microneme protein which plays an indispensable role during Apicomplexa parasite invasion. The detailed mechanism of AMA-1 molecular interaction with its receptor on bovine erythrocytes has not been completely defined in Babesia bovis. This study was focused on identifying the minimum B. bovis AMA-1-derived regions governing specific and high-affinity binding to its target cells. Different approaches were used for detecting ama-1 locus genetic variability and natural selection signatures. The binding properties of twelve highly conserved 20-residue-long peptides were evaluated using a sensitive and specific binding assay based on radio-iodination. B. bovis AMA-1 ectodomain structure was modelled and refined using molecular modelling software. NetMHCIIpan software was used for calculating B- and T-cell epitopes. The B. bovis ama-1 gene had regions under functional constraint, having the highest negative selective pressure intensity in the Domain I encoding region. Interestingly, B. bovis AMA-1-DI (100YMQKFDIPRNHGSGIYVDLG119 and 120GYESVGSKSYRMPVGKCPVV139) and DII (302CPMHPVRDAIFGKWSGGSCV321)-derived peptides had high specificity interaction with erythrocytes and bound to a chymotrypsin and neuraminidase-treatment sensitive receptor. DI-derived peptides appear to be exposed on the protein’s surface and contain predicted B- and T-cell epitopes. These findings provide data (for the first-time) concerning B. bovis AMA-1 functional subunits which are important for establishing receptor-ligand interactions which could be used in synthetic vaccine development. 相似文献
2.
To advance organ-on-a-chip development and other areas befitting from physiologically-relevant biomembranes,a microfluidic platform is presented for synthesis of biomembranes during gelation and investigation into their role as extracellular matrix supports.In this work,high-throughput studies of collagen,chitosan,and collagen-chitosan hybrid biomembranes were carried out to characterize and compare key properties as a function of the applied hydrodynamic conditions during gelation.Specifically,depending on the biopolymer material used,varying flow conditions during biomembrane gelation caused width,uniformity,and swelling ratio to be differently affected and controllable.Finally,cell viability studies of seeded fibroblasts were conducted,thus showing the potential for biological applications. 相似文献
3.
Vanessa Modelski Schatkoski Thaís Larissa do Amaral Montanheiro Beatriz Rossi Canuto de Menezes Raissa Monteiro Pereira Karla Faquine Rodrigues Renata Guimarães Ribas Diego Morais da Silva Gilmar Patrocínio Thim 《Ceramics International》2021,47(3):2999-3012
Studies related to biomaterials that stimulate the repair of living tissue have increased considerably, improving the quality of many people's lives that require surgery due to traumatic accidents, bone diseases, bone defects, and reconstructions. Among these biomaterials, bioceramics and bioactive glasses (BGs) have proved to be suitable for coating materials, cement, scaffolds, and nanoparticles, once they present good biocompatibility and degradability, able to generate osteoconduction on the surrounding tissue. However, the role of biomaterials in hard tissue engineering is not restricted to a structural replacement or for guiding tissue regeneration. Nowadays, it is expected that biomaterials develop a multifunctional role when implanted, orchestrating the process of tissue regeneration and providing to the body the capacity to heal itself. In this way, the incorporation of specific metal ions in bioceramics and BGs structure, including magnesium, silver, strontium, lithium, copper, iron, zinc, cobalt, and manganese are currently receiving enhanced interest as biomaterials for biomedical applications. When an ion is incorporated into the bioceramic structure, a new category of material is created, which has several unique properties that overcome the disadvantages of primitive material and favors its use in different biomedical applications. The doping can enhance handling properties, angiogenic and osteogenic performance, and antimicrobial activity. Therefore, this review aims to summarize the effect of selected metal ion dopants into bioceramics and silicate-based BGs in bone tissue engineering. Furthermore, new applications for doped bioceramics and BGs are highlighted, including cancer treatment and drug delivery. 相似文献
4.
Russian Engineering Research - The commercialization of intellectual property at high-tech enterprises is discussed. The participants in commercialization are identified. A three-component matrix... 相似文献
5.
Yuri Prozzillo Stefano Cuticone Diego Ferreri Gaia Fattorini Giovanni Messina Patrizio Dimitri 《International journal of molecular sciences》2021,22(9)
Chromatin organization is developmentally regulated by epigenetic changes mediated by histone-modifying enzymes and chromatin remodeling complexes. In Drosophila melanogaster, the Tip60 chromatin remodeling complex (dTip60) play roles in chromatin regulation, which are shared by evolutionarily-related complexes identified in animal and plants. Recently, it was found that most subunits previously assigned to the dTip60 complex are shared by two related complexes, DOM-A.C and DOM-B.C, defined by DOM-A and DOM-B isoforms, respectively. In this work, we combined classical genetics, cell biology, and reverse genetics approaches to further investigate the biological roles played during Drosophila melanogaster development by a number of subunits originally assigned to the dTip60 complex. 相似文献
6.
Martinez-Yelmo Isaias Alvarez-Horcajo Joaquin Briso-Montiano Miguel Lopez-Pajares Diego Rojas Elisa 《Telecommunication Systems》2019,72(4):555-565
Telecommunication Systems - The Software-Defined Networking (SDN) architecture decouples the control plane from the data plane, but it does not explicitly state where the control should be located.... 相似文献
7.
ABSTRACTConception and development of an Unmanned Aerial Vehicle (UAV) capable of detecting, tracking and following a moving object with unknown dynamics is presented in this work, considering a human face as a case of study. Object detection is accomplished by a Haar cascade classifier. Once an object is detected, it is tracked with the help of a Kalman Filter (KF), and an estimation of the relative position with respect to the target is obtained. A linear controller is used to validate the proposed vision scheme and for regulating the aerial robot's position in order to keep a constant distance with respect to the mobile target, employing as well the extra available information from the embedded sensors. The proposed system was extensively tested in real-time experiments, through different conditions, using a commercial quadcopter connected via wireless to a ground station running under the Robot Operative System (ROS). The proposed overall strategy shows a good performance even under disadvantageous conditions as outdoor flight, being robust against illumination changes, image noise and the presence of other people in the scene. 相似文献
8.
Diego Nocetti Hipólito Núñez Luis Puente Alejandra Espinosa Fernando Romero 《Journal of the science of food and agriculture》2020,100(12):4335-4346
Goldenberry is a wild fruit that has been widely used for centuries, mainly in folk medicine. Most studies of goldenberry have focused on the fruit, but new research has studied its byproducts, which were considered to be waste until recently. The main objective of our study was to systematize the published information regarding the composition of goldenberry byproducts (calyces, leaves, seeds, and pomace) and their effects on biological systems. Goldenberry byproducts contain minerals, amino acids, withanolides, flavonoids, and essential fatty acids, thus representing good sources of these compounds. Some of their major biological effects include anti-inflammatory, antioxidant, antidiabetic, and antiproliferative effects. Information regarding their toxicity is also presented here. To determine the optimal dosage, further safety studies would be recommended to ensure the best health benefits of these compounds. The available evidence has demonstrated the nutritional value of different byproducts of goldenberry, suggesting them to be potential candidates for use in the cosmetic industry, in the preparation of functional foods, and in phytomedicine for the prevention and adjuvant treatment of some diseases. © 2020 Society of Chemical Industry 相似文献
9.
Jorge Pascual-Fernndez Alejandro Fernndez-Montero Alfredo Crdova-Martínez Diego Pastor Alejandro Martínez-Rodríguez Enrique Roche 《International journal of molecular sciences》2020,21(22)
Aging is associated with sarcopenia. The loss of strength results in decreased muscle mass and motor function. This process accelerates the progressive muscle deterioration observed in older adults, favoring the presence of debilitating pathologies. In addition, sarcopenia leads to a decrease in quality of life, significantly affecting self-sufficiency. Altogether, these results in an increase in economic resources from the National Health Systems devoted to mitigating this problem in the elderly, particularly in developed countries. Different etiological determinants are involved in the progression of the disease, including: neurological factors, endocrine alterations, as well as nutritional and lifestyle changes related to the adoption of more sedentary habits. Molecular and cellular mechanisms have not been clearly characterized, resulting in the absence of an effective treatment for sarcopenia. Nevertheless, physical activity seems to be the sole strategy to delay sarcopenia and its symptoms. The present review intends to bring together the data explaining how physical activity modulates at a molecular and cellular level all factors that predispose or favor the progression of this deteriorating pathology. 相似文献
10.