Fluorescence Correlation Spectroscopy Analysis of Effect of Molecular Crowding on Self-Assembly of β-Annulus Peptide into Artificial Viral Capsid

Recent progress in the de novo design of self-assembling peptides has enabled the construction of peptide-based viral capsids. Previously, we demonstrated that 24-mer β-annulus peptides from tomato bushy stunt virus spontaneously self-assemble into an artificial viral capsid. Here we propose to use the artificial viral capsid through the self-assembly of β-annulus peptide as a simple model to analyze the effect of molecular crowding environment on the formation process of viral capsid. Artificial viral capsids formed by co-assembly of fluorescent-labelled and unmodified β-annulus peptides in dilute aqueous solutions and under molecular crowding conditions were analyzed using fluorescence correlation spectroscopy (FCS). The apparent particle size and the dissociation constant (K_d) of the assemblies decreased with increasing concentration of the molecular crowding agent, i.e., polyethylene glycol (PEG). This is the first successful in situ analysis of self-assembling process of artificial viral capsid under molecular crowding conditions.     

Full scale investigation of GCL damage mechanisms in small earth dam retrofit applications under earthquake loading

This paper reports results of full scale testing to further explore potential GCL damage mechanisms in earth dam retrofit applications in seismically active areas; in particular, to a) investigate whether shear displacements could reduce the magnitude of GCL panel overlap during earthquake shaking; b) explore the influence of gravel particles on GCL thickness at localised point of contact; and c) observe the consequences of an accidental exposure of an uncovered GCL to short duration rainfall in terms of moisture content and effects during subsequent compaction. The results of these experiments indicate that even under severe shaking no movements were detected at the GCL panel overlap. Whereas gravel particles were observed to locally reduce the thickness of the GCL to 2.2 mm, no plowing of the particle into the GCL occurred due to a lack of shear displacement at the interface, resulting in no localised internal erosion through the barrier. Furthermore, hydration of GCL panels during construction due to surface wetting was observed to result in a state of hydration less than its post-construction state. These results indicate that although each of the three GCL damage mechanisms cannot be ruled out to ever be relevant in practice, the performance of the GCL retrofitted earth dam tested was satisfactory under even severe Level 2 earthquake shaking, and suggests that the retrofitting of small earth dams with GCLs is a promising strategy to improve their static and seismic resistance.     

Possibility of Japanese Cedar Pollen Causing False Positives in the Deep Mycosis Test

Because Japanese cedar pollen (JCP) contains beta-1,3-d-glucan (BG), there is concern that its lingering presence in the atmosphere, especially during its scattering period, may cause false positives in the factor-G-based Limulus amebocyte lysate (LAL) assay used to test for deep mycosis (i.e., G-test). Hence, we examined whether the LAL assay would react positively with substances contained in JCP by using the G-test to measure JCP particles and extracts. BG was purified from the JCP extract on a BG-specific affinity column, and the percentage extractability was measured using three different BG-specific quantitative methods. The G-test detected 0.4 pg BG in a single JCP particle and 10 fg from a single particle in the extract. The percentage extractability of JCP-derived BG was not significantly different among the three quantitative methods. As the JCP particles should technically have been removed during serum separation, they should be less likely to be a direct false-positive factor. However, given that the LAL-assay-positive substances in the JCP extract were not distinguishable by the three BG-specific quantitative methods, we conclude that they may cause the background to rise. Therefore, in Japan false positives arising from JCP contamination should be considered when testing patients for deep mycosis.     

Learning subtree pattern importance for Weisfeiler-Lehman based graph kernels

Machine Learning - Graph is an usual representation of relational data, which are ubiquitous in many domains such as molecules, biological and social networks. A popular approach to learning with...     

The effects of warmth and CO2 concentration,with and without bioeffluents,on the emission of CO2 by occupants and physiological responses

The emission rate of carbon dioxide (CO₂) depends on many factors but mainly on the activity level (metabolic rate) of occupants. In this study, we examined two other factors that may influence the CO₂ emission rate, namely the background CO₂ concentration and the indoor temperature. Six male volunteers sat one by one in a 1.7 m³ chamber for 2.5 h and performed light office-type work under five different conditions with two temperature levels (23 vs. 28°C) and three background concentrations of CO₂ (800 vs. 1400 vs. 3000 ppm). Background CO₂ levels were increased either by dosing CO₂ from a cylinder or by reducing the outdoor air supply rate. Physiological responses to warmth, added CO₂, and bioeffluents were monitored. The rate of CO₂ emission was estimated using a mass-balance equation. The results indicate a higher CO₂ emission rate at the higher temperature, at which the subjects were warm, and a lower emission rate in all conditions in which the background CO₂ concentration increased. Physiological measurements partially explained the present results but more measurements are needed.     

广州市老年人使用空气调节设备状况及对健康影响调查

老人的健康与建筑室内环境存在重要联系，为了给适老建筑的暖通系统优化设计提供理论依据，有必要探究室内空气调节设备与老人健康的关系。对广州市104位本地老年人进行了问卷调查，收集了老年人的基本健康信息和家庭使用空气调节设备的情况，包括制冷、除湿、采暖和空气净化4种设备及其使用时长，使用习惯。收集到的信息用SPSS20.0进行了统计分析。结果包括广州市老年人家中四种设备拥有率、设备平均使用龄、设备使用时间以及常见日均使用时长；所调查的老年人健康比例达到55.8%。患病老人中心血管疾病的比例最高，其余为风湿性疾病和关节炎等疾病。相关性分析表明，广州地区夏天家庭采取制冷措施的老人患病可能性更小；制冷设备常见日均使用时长与身处空调区域时长的比值越大，老人患病的可能性越小。     

Compositional Dependence of Normal Spectral Emissivity of Molten Cu-Fe Alloy

Metallurgical and Materials Transactions B - Normal spectral emissivity of molten Cu-Fe alloy with different compositions was measured at the wavelength of 807 nm using an electromagnetic...     

Dynamic Stabilization of DNA Assembly by Using Pyrrole-Imidazole Polyamide

We used N-methylpyrrole (Py)-N-methylimidazole-(Im) polyamide as an exogenous agent to modulate the formation of DNA assemblies at specific double-stranded sequences. The concept was demonstrated on the hybridization chain reaction that forms linear DNA. Through a series of melting curve analyses, we demonstrated that the binding of Py−Im polyamide positively influenced both the HCR initiation and elongation steps. In particular, Py−Im polyamide was found to drastically stabilize the DNA duplex such that its thermal stability approached that of an equivalent hairpin structure. Also, the polyamide served as an anchor between hairpin pairs in the HCR assembly, thus improving the originally weak interstrand stability. We hope that these proof-of-concept results can inspire future use of Py−Im polyamide as a molecular tool to modulate the formation of DNA assemblies.