全文获取类型
收费全文 | 582篇 |
免费 | 12篇 |
国内免费 | 1篇 |
专业分类
电工技术 | 54篇 |
综合类 | 1篇 |
化学工业 | 125篇 |
金属工艺 | 23篇 |
机械仪表 | 12篇 |
建筑科学 | 3篇 |
能源动力 | 26篇 |
轻工业 | 30篇 |
无线电 | 59篇 |
一般工业技术 | 87篇 |
冶金工业 | 102篇 |
原子能技术 | 36篇 |
自动化技术 | 37篇 |
出版年
2023年 | 3篇 |
2022年 | 2篇 |
2021年 | 3篇 |
2020年 | 7篇 |
2019年 | 4篇 |
2018年 | 10篇 |
2017年 | 5篇 |
2016年 | 9篇 |
2015年 | 7篇 |
2014年 | 9篇 |
2013年 | 22篇 |
2012年 | 16篇 |
2011年 | 22篇 |
2010年 | 19篇 |
2009年 | 26篇 |
2008年 | 23篇 |
2007年 | 28篇 |
2006年 | 14篇 |
2005年 | 24篇 |
2004年 | 20篇 |
2003年 | 18篇 |
2002年 | 19篇 |
2001年 | 11篇 |
2000年 | 10篇 |
1999年 | 15篇 |
1998年 | 51篇 |
1997年 | 29篇 |
1996年 | 25篇 |
1995年 | 13篇 |
1994年 | 10篇 |
1993年 | 16篇 |
1992年 | 6篇 |
1991年 | 2篇 |
1990年 | 7篇 |
1989年 | 6篇 |
1988年 | 4篇 |
1987年 | 7篇 |
1986年 | 6篇 |
1985年 | 7篇 |
1984年 | 5篇 |
1983年 | 7篇 |
1982年 | 7篇 |
1981年 | 4篇 |
1980年 | 8篇 |
1979年 | 12篇 |
1978年 | 3篇 |
1977年 | 4篇 |
1976年 | 2篇 |
1973年 | 4篇 |
1960年 | 1篇 |
排序方式: 共有595条查询结果,搜索用时 390 毫秒
1.
Yasuha Watanabe Prof. Kenzo Fujimoto 《Chembiochem : a European journal of chemical biology》2020,21(22):3244-3248
The regulation of DNAzyme activity is an important problem for its in vivo applications. We achieved photochemical regulation of DNAzyme activity by using reversible DNA photo-crosslinking of 3-cyanovinylcarbazole (CNVK). The ODN containing CNVK photo-crosslinked to a pyrimidine base in the complementary strand after a few seconds of photoirradiation, and its photoadduct was split by photoirradiation of another wavelength. The activity of photo-crosslinked DNAzyme with CNVK was completely inhibited (OFF state). In contrast, after 312 nm irradiation, DNAzyme activity was recovered upon addition of a substrate strand (ON state). In addition, the photo-crosslinked DNAzyme is prone to enzymatic digestion by exonuclease. This photochemical OFF to ON switching with reversible DNA photo-crosslinking was regulated at the desired time and position; therefore, it might be possible to use it for in vivo application. 相似文献
2.
3.
4.
In this paper, photoresponsive behavior of multi-bilayered films having precisely controlled layer thickness prepared by stacking an azo-functionalized polymer liquid crystal, PMAzXAc, and polyvinyl alcohol alternatively, PVA, is described. The multi-bilayered films were found to reflect a light of specific wavelength depending on the layer thickness and refractive index, and showed the reversible change in the reflection intensity by irradiation with visible and UV lights. The change in the reflection intensity was brought about by change in the molecular orientation of PMAzXAc between an out-of-plane orientation and a photo-induced isotropic state, and was strongly dependent on the number of methylene spacer of PMAzXAc linking the azobenzene side group with the acrylate polymer main chain. PMAz6Ac with hexa-methylene spacer showed the largest change in the reflection intensity, while smaller change in the reflection intensity was observed for PMAzXAc having shorter or longer methylene spacer than 6. The effect of the methylene spacers on the photochemical change in the molecular orientation of azobenzene chromophores in the multi-bilayered films will be discussed. 相似文献
5.
Takatoshi Shindo Hideki Motoyama Toru Miki Mikihisa Saito Akiyori Matsueda Noriyasu Honma Tomohito Hida Kazuo Shinjo Kiyotaka Hayashi Hayato Awazu Katsuhisa Makabe Masato Fujikawa Satoshi Kurihara Masashi Sato 《IEEJ Transactions on Electrical and Electronic Engineering》2016,11(Z2):S28-S33
Nine electric power companies in Japan have been collecting lightning data with lightning location systems. Five years of the lightning data (2009–2013) are summarized and analyzed in this paper. The recent annual number of lightning flashes of which the current is more than 10 kA in Japan is around 1 million. The variations of lightning occurrence characteristics by areas, seasons, and so on, are clarified. Meteorological conditions that may affect lightning occurrence are also discussed. Furthermore, we show that there is a clear correlation between the number of lightning strikes and the outage rate of transmission lines. © 2016 Institute of Electrical Engineers of Japan. Published by John Wiley & Sons, Inc. 相似文献
6.
Supramolecular Ligands for Histone Tails by Employing a Multivalent Display of Trisulfonated Calix[4]arenes 下载免费PDF全文
Dr. Yasuaki Kimura Nae Saito Kayo Hanada Jiaan Liu Prof. Takayoshi Okabe Dr. Shigehiro A. Kawashima Dr. Kenzo Yamatsugu Prof. Motomu Kanai 《Chembiochem : a European journal of chemical biology》2015,16(18):2599-2604
Post‐translational modification of histone tails plays critical roles in gene regulation. Thus, molecules recognizing histone tails and controlling their epigenetic modification are desirable as biochemical tools to elucidate regulatory mechanisms. There are, however, only a few synthetic ligands that bind to histone tails with substantial affinity. We report CA2 and CA3, which exhibited sub‐micromolar affinity to histone tails (especially tails with a trimethylated lysine). Multivalent display of trisulfonated calix[4]arene was important for strong binding. CA2 was applicable not only to synthetic tail peptides but also to endogenous histone proteins, and was successfully used to pull‐down endogenous histones from nuclear extract. These findings indicate the utility of these supramolecular ligands as biochemical tools for studying chromatin regulator protein and as a targeting motif in ligand‐directed catalysis to control epigenetic modifications. 相似文献
7.
Xuetao Shi Serge Ostrovidov Yihua Zhao Xiaobin Liang Motohiro Kasuya Kazue Kurihara Ken Nakajima Hojae Bae Hongkai Wu Ali Khademhosseini 《Advanced functional materials》2015,25(15):2250-2259
Engineering living tissues that simulate their natural counterparts is a dynamic area of research. Among the various models of biological tissues being developed, fiber‐shaped cellular architectures, which can be used as artificial blood vessels or muscle fibers, have drawn particular attention. However, the fabrication of continuous microfiber substrates for culturing cells is still limited to a restricted number of polymers (e.g., alginate) having easy processability but poor cell–material interaction properties. Moreover, the typical smooth surface of a synthetic fiber does not replicate the micro‐ and nanofeatures observed in vivo, which guide and regulate cell behavior. In this study, a method to fabricate photocrosslinkable cell‐responsive methacrylamide‐modified gelatin (GelMA) fibers with exquisite microstructured surfaces by using a microfluidic device is developed. These hydrogel fibers with microgrooved surfaces efficiently promote cell encapsulation and adhesion. GelMA fibers significantly promote the viability of cells encapsulated in/or grown on the fibers compared with similar grooved alginate fibers used as controls. Importantly, the grooves engraved on the GelMA fibers induce cell alignment. Furthermore, the GelMA fibers exhibit excellent processability and could be wound into various shapes. These microstructured GelMA fibers have great potential as templates for the creation of fiber‐shaped tissues or tissue microstructures. 相似文献
8.
9.
For a large volume air cleanup system in nuclear fusion facility honeycomb type oxidizing catalyst offers more useful advantage in terms of their low pressure drop than the conventional particle packed bed. Scaling up examination of 35 times large volume Pd catalyst deposit on metal honeycomb Al–Cr-steel alloy had been performed comparing with fundamental small size honeycomb. The catalyst was heated up to over 400 °C with stepwise by combined the inner and external heaters under various flow rate of processing gas containing various concentrations of hydrogen and methane. Then oxidizing reaction rates and the catalytic activation energies were evaluated and thermal property of catalyst bed was estimated considering exothermic oxidizing reaction in the process gas. As a result it was revealed that the scaling up honeycomb catalyst bed would be practically useful for the air cleanup system. 相似文献
10.
F. Yuasa E. de Doncker N. Hamaguchi T. Ishikawa K. Kato Y. Kurihara J. Fujimoto Y. Shimizu 《Computer Physics Communications》2012,183(10):2136-2144
A new approach is presented to evaluate multi-loop integrals, which appear in the calculation of cross-sections in high-energy physics. It relies on a fully numerical method and is applicable to a wide class of integrals with various mass configurations. As an example, the computation of two-loop planar and non-planar box diagrams is shown. The results are confirmed by comparisons with other techniques, including the reduction method, and by a consistency check using the dispersion relation. 相似文献