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Fluorescence‐Activated Cell Sorters: Standing Surface Acoustic Wave (SSAW)‐Based Fluorescence‐Activated Cell Sorter (Small 40/2018) 下载免费PDF全文
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Liqiang Ren Shujie Yang Peiran Zhang Zhiguo Qu Zhangming Mao Po‐Hsun Huang Yuchao Chen Mengxi Wu Lin Wang Peng Li Tony Jun Huang 《Small (Weinheim an der Bergstrasse, Germany)》2018,14(40)
Microfluidic fluorescence‐activated cell sorters (μFACS) have attracted considerable interest because of their ability to identify and separate cells in inexpensive and biosafe ways. Here a high‐performance μFACS is presented by integrating a standing surface acoustic wave (SSAW)‐based, 3D cell‐focusing unit, an in‐plane fluorescent detection unit, and an SSAW‐based cell‐deflection unit on a single chip. Without using sheath flow or precise flow rate control, the SSAW‐based cell‐focusing technique can focus cells into a single file at a designated position. The tight focusing of cells enables an in‐plane‐integrated optical detection system to accurately distinguish individual cells of interest. In the acoustic‐based cell‐deflection unit, a focused interdigital transducer design is utilized to deflect cells from the focused stream within a minimized area, resulting in a high‐throughput sorting ability. Each unit is experimentally characterized, respectively, and the integrated SSAW‐based FACS is used to sort mammalian cells (HeLa) at different throughputs. A sorting purity of greater than 90% is achieved at a throughput of 2500 events s?1. The SSAW‐based FACS is efficient, fast, biosafe, biocompatible and has a small footprint, making it a competitive alternative to more expensive, bulkier traditional FACS. 相似文献
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Yu‐Jung Lin Chun‐Chieh Chen Dang Nguyen Huei‐Rou Su Kun‐Ju Lin Hsin‐Lung Chen Yu‐Jung Hu Po‐Liang Lai Hsing‐Wen Sung 《Small (Weinheim an der Bergstrasse, Germany)》2020,16(23)
Nitric oxide (NO) is a potent tumor‐cell radiosensitizer but it can be readily scavenged by hemoglobin (Hb) in vivo. A biomimetic incubator that can generate and deliver NO in a scavenger (Hb)‐free environment to enhance its radiosensitizing effect to maximize its efficacy in radiotherapy is proposed. This NO incubator comprises a poly(lactic‐co‐glycolic acid) (PLGA) hollow microsphere (HM) that contains an NO donor (NONOate) and a surfactant molecule (sodium caprate, SC) in its aqueous core. In acidic tumorous environments, the PLGA shell of the HM allows the penetration of protons from the outside, activating the hydrolytic cleavage of NONOate, spontaneously generating NO bubbles, which are immediately trapped/stabilized by SC. The SC‐stabilized NO bubbles in the HM are then squeezed through the spaces of its PLGA matrices by the elevated internal pressure. Upon leaving the HM, the entrapped NO molecules may passively diffuse through their SC‐stabilized/protected layer gradually to the tumor site, having a long‐lasting radiosensitizing effect and inhibiting tumor growth. The entire process of NO generation and delivery is conducted in a scavenger (Hb)‐free environment, mimicking the development of young ovoviviparous fish inside their mothers' bodies in the absence of predators before birth. 相似文献
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高校体育馆在教学、管理及使用功能等方面不同于社会体育馆.通过对南阳师范学院体育馆建筑设计分析,从环境关联性、多功能综合性、文化延续性三个方面,探讨了高校体育馆的建筑设计特点及发展趋势. 相似文献
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