Interaction of IL-22/IL-22R1 promotes cell proliferation and suppresses apoptosis of colorectal cancer via phosphorylation of STAT3

Interleukin-22 (IL-22) is a member of IL-10 cytokine family which is expressed in activated T cells predominantly and in activated natural killer cells at lower levels. Previous studies have demonstrated the link between elevated levels of IL-22 and disease severity of psoriasis, Crohn’s disease, rheumatoid arthritis and interstitial lung diseases. However, the function of IL-22 in the development and progression of colorectal cancer (CRC) remains elusive. In this study, we first evaluated the IL-22/IL-22R1 level in CRC patients, and found that tumor tissues have more active expression of IL-22 and IL-22R1 than normal tissues, presenting correlation with the degree of differentiation of tumor tissues. Subsequently, Caspase and cell viability assays were performed on SW-480 cell line which expresses high level of IL-22R1 to examine if the supplementation of IL-22 has an impact on apoptosis and proliferation. In comparison with treatment of 5-FU, supplementation of IL-22 promoted cell proliferation and ameliorated apoptosis. To unveil signal transduction upon activation of IL-22R, we examined the phosphorylation of STAT3 in SW-480 cell line following supplementation of IL-22. The treatment of IL-22 also increased the level of p-Akt, an essential component in PI3K/Akt pathway. Although the link between STAT3 phosphorylation and PI3K/ Akt activation remains to be explored, our study revealed the mechanism underlying the effects of IL-22R activation on apoptosis as well as tumor differentiation, indicating the prognostic value of IL-22/IL-22R.     

受潮汐影响的深基坑放坡开挖与回筑施工技术

以台州港头门港某港区煤污水池深基坑为依托,在难以进行降水且由于回填宕石而无法施打拉森钢板桩的情况下,研究放坡开挖的可行性.在考虑潮汐作用时间性与空间性的同时,研究了不降水情况下赶潮开挖与赶潮结构回筑的施工措施,形成了一套适用于受潮汐影响显著的邻海地区深基坑施工技术.     

低GWP制冷剂在热泵行业的应用分析

随着世界各国对于环境问题的日益关注,热泵行业中的R22制冷剂产品已经开始逐步降低使用,主流制冷剂R134a、R410A等已经被列入受控物质范围,使用更低GWP的制冷剂成为热泵行业可持续发展的重要支撑.针对现有热泵行业的三大主流细分领域高温热泵、普通热泵、低温环境热泵,分别对各个领域的低GWP制冷剂的应用情况进行分析,提出未来中期和长期的可能发生的冷媒替换形势.同时对R290和CO2两种典型的适用于热泵行业的天然制冷剂进行探讨,分析其未来发展趋势.开发适应于我国国情又能在国际竞争中处于优势的全新低GWP制冷剂(包括天然制冷剂)的热泵产品或将成为行业后续发展方向.     

海底隧道钢筋混凝土基于氯盐腐蚀的耐久性参数设计研究

胶州湾海底隧道是中国自行建造的第二条海底隧道,其服役寿命为100a。在详细分析海底隧道结构混凝土的服役环境基础上,参考国内外现有的寿命预测模型,依据混凝土耐久性试验结果和重大工程类比,综合考虑混凝土碳化、混凝土初始氯离子浓度影响等因素,建立了海底隧道混凝土在双重破坏因素作用下的寿命预测模型。通过上述模型进行计算,计算结果表明:(1)海底隧道要达到100a服役寿命,其衬砌混凝土靠近空气一侧保护层厚度应大于70mm,靠近土体一侧应大于60mm;(2)混凝土初始氯离子浓度应小于0.15%;(3)氯离子扩散系数应小于4×10-12m2/s,水胶比w/b应小于0.34,混凝土强度等级应高于C50。此外,依据混凝土耐久性能与其配合比、原材料之间的定量关系,提出海底隧道100a服役寿命的混凝土配合比设计和原材料优选原则。     

基于高精度电感法的无刷直流电机起动及反电动势同步检测稳定性研究

提出了一种基于电感法的高精度定位方案,在不增加定位电流脉冲数量的基础上将定位精度由原先的60°电角度提高至30°电角度,并消除了电机因工艺问题导致的相绕组参数不一致对检测的影响。设计了一种新颖的切换算法,可在极短时间内稳定可靠地切换到闭环。讨论了反电动势的同步检测方案,通过理论分析和仿真对比了PWM导通和PWM关断时刻检测反电动势过零信号方法的区别,并对干扰因素提出了补偿方案。样机实验验证了所提方案的稳定性和可靠性。     

计及样本容量合理性的风电功率预测考核算法

在风功率预测误差服从正态分布的基础上,首先,提出了在给定置信水平、最大允许误差下最小样本容量的计算方法,以及在给定误差范围、样本容量下估计值置信水平的计算方法。其次,针对典型显著性水平、误差范围进行分析,论证了当样本容量较小时,样本统计指标置信水平低,无法代表总体指标。最后,提出了计及样本容量合理性的日前风电功率预测准确率考核算法,实际算例表明,所提考核算法更科学合理,避免了不合理的考核罚款。     

深部大变形软岩巷道返修支护对策研究

淮南矿业集团望峰岗矿-817水平内水仓埋深约850 m,虽经多次返修,但其围岩难以维护稳定.根据地应力场和巷道变形破坏原因的分析,对该水仓巷道原支护方案进行了调整,在返修支护方案中增加了底部反拱,并采用钢纤维混凝土喷层.利用FLAC软件的用户自定义损伤本构模型（UDM）对无支护、原支护方案以及返修支护方案的深部开挖损伤区进行了数值分析,结果表明返修支护方案可以减小开挖损伤区.现场试验结果表明,返修支护方案能够维持围岩巷道的稳定.     

低碳低合金及含铜海洋工程用钢的研究及其发展

随着海洋资源的不断开采和利用,对深海及极地用海洋工程关键部位用钢强韧性等综合性能提出了更高的要求;开发具有更高强塑性,低屈强比,良好的低温韧性、焊接性以及耐腐蚀性等综合性能优良的钢材是海洋工程用钢的发展方向。介绍了国内外低碳低合金海洋工程用钢的研究和发展概况,分析了该类钢种制造过程中面临的问题,对铜在低碳高强钢中的作用以及含铜海洋工程用钢的研发情况进行了综述。     

Synthesis of KUE-siRNA Conjugates for Prostate Cancer Cell-Targeted Gene Silencing

The delivery of siRNAs to selectively target cells poses a great challenge in RNAi-based cancer therapy. The lack of suitable cell-targeting methods seriously restricts the advance in delivering siRNAs to extrahepatic tissues. Based on prostate-specific membrane antigen (PSMA)-targeting ligands, we have synthesized a series of lysine-urea-glutamate (KUE)-siRNA conjugates and verified their effective cell uptake and gene silencing properties in prostate cancers. The results indicated that the KUE-siRNA conjugates could selectively enter PSMA⁺ LNCaP cells, eventually down-regulating STAT3 expression. Based on post-synthesis modification and receptor-mediated endocytosis, this strategy of constructing ligand-siRNA conjugates might provide a general method of siRNA delivery for cell-targeted gene silencing.     

Fabrication of Co doped MoS2 nanosheets with enlarged interlayer spacing as efficient and pH-Universal bifunctional electrocatalyst for overall water splitting

Exploring inexpensive and active bifunctional electrocatalysts to produce hydrogen and oxygen from water at all pHs is highly desirable. Herein, we report a facile one-step method to prepare vertically aligned Co doped MoS₂ nanosheets with extended interlayer distance on carbon cloth (Co–MoS₂@CC) for full hydrolysis in both alkaline and acidic medium. Co–MoS₂@CC exhibits long-term durability with overpotentials of 56.6 mV and 130 mV for hydrogen generation and 242 mV and 201 mV for oxygen production at 10 mA cm^?2 in basic and acidic conditions, respectively. Moreover, we achieve low voltages of 1.585 V and 1.55 V in basic and acidic conditions respectively for the overall water splitting. We assume that such excellent property of Co–MoS₂@CC may be ascribed to the uncovering of more active sites and high porosity resulted from Co doping, which boosts the conductivity and thus reduces MoS₂ hydrogen adsorption free energy in HER, as well as benefits to catalytic active sites in OER. This one-step doping approach opens up new ways to regulate the intrinsic catalytic activity to catalyze total hydrolysis at all PHs.