Photon Upconversion Hydrogels for 3D Optogenetics

The ability to optically induce biological responses in 3D has been dwarfed by the physical limitations of visible light penetration to trigger photochemical processes. However, many biological systems are relatively transparent to low-energy light, which does not provide sufficient energy to induce photochemistry in 3D. To overcome this challenge, hydrogels that are capable of converting red or near-IR (NIR) light into blue light within the cell-laden 3D scaffolds are developed. The upconverted light can then excite optically active proteins in cells to trigger a photochemical response. The hydrogels operate by triplet–triplet annihilation upconversion. As proof-of-principle, it is found that the hydrogels trigger an optogenetic response by red/NIR irradiation of HeLa cells that have been engineered to express the blue-light sensitive protein Cry2olig. While it is remarkable to photoinduce the clustering of Cry2olig with blanket NIR irradiation in 3D, it is also demonstrated how the hydrogels trigger clustering within a single cell with great specificity and spatiotemporal control. In principle, these hydrogels may allow for photochemical control of cell function within 3D scaffolds, which can lead to a wealth of fundamental studies and biochemical applications.     

Thermal performance of non air-conditioned buildings with vaulted roofs in comparison with flat roofs

Vaulted or domed roofs have been frequently adopted by builders and architects throughout the Middle East and other hot dry areas. However, the thermal performance of such buildings under hot dry climatic conditions has rarely been quantitatively studied. In this paper, a detailed finite element model for the investigation of the thermal performance of non air-conditioned buildings with vaulted roofs (VR) is suggested based on two-dimensional unsteady heat transfer in such roofs and solar geometry. This model allows a comparison of the thermal performance of non air-conditioned buildings with a VR and a flat roof (FR) under different climatic conditions. Results obtained by numerical calculation show that, irrespective of building type the VRs are applied to, buildings with a VR have lower indoor temperatures as compared to those with a FR. The reason is that such roofs dissipate more heat than a FR does by convection and thermal radiation at night due to the enlarged curved surfaces. This implies that such roof forms are suitable for buildings located in hot dry regions but not for those located in hot humid areas, and reasonably explains why curved roofs have been extensively adopted by builders and architects in the hot dry areas in the past. However, with the decrease in the half rim angle of a VR, the difference of indoor thermal condition between a VR and a FR building becomes small and insignificant. Results also indicate that the indoor air temperature is slightly influenced by the half rim angle _θ0${\theta }_0$ and the orientation _φv${\phi }_v$ of the VR. To be effective to create a favorable thermal condition inside buildings with a VR under hot dry climatic conditions, the half rim angle of a VR should be θ₀>50°, instead of _θ0<50^°${\theta }_0<50^{°}$, which is the optimal half rim angle of a VR of air-conditioned buildings, as found by the present authors in a previous study.     

Interaction between the mixing and displacement modes in a naturally ventilated enclosure

Experiments are carried out to study for the first time interactive phenomena in buoyancy-induced natural ventilation in a full-scale enclosure with upper and lower openings on one of the sidewalls. The interaction between the mixing and the displacement ventilation modes is revealed by opening the lower vent to different heights while the upper vent is kept fully open. Both the transient process and steady state interaction are explored. Measurements include temperature differences between inside and outside and air velocity through the upper opening. The level of the neutral plane at the upper vent, defined here as the plane separating between inflow and outflow, decreases with R^*$R^{*}$, the ratio between the opening heights (and areas) of the lower and upper vents. Experiments show that when 0*<0.27$0<R^{*}<0.27$ the mixing and displacement modes interact through a new combined ventilation mode. For 0.53*?1$0.53<R^{*}?1$, the displacement mode dominates whereas in the intermediate range, 0.27?R^*?0.53$0.27?R^{*}?0.53$, either the combined or the pure displacement mode takes place. The experiments are in qualitative agreement with a previous theoretical model.     

Mechanism of Infrared Detection and Transduction by Beetle Melanophila Acuminata In memory of Jerry Wolken

The Melanophila acuminata beetle is attracted to forest fires via a pair of infrared sensory organs composed of sensilla. Our histological work showed that each sensillum contains lipid layers surrounding a protein layer and a unique polysaccharide base that is associated with a neuron to each sensillum. Infrared microscopy showed that the protein region maximally absorbs infrared radiation at 3 μm wavelength and at 10 μm, which corresponds to the known radiation produced by forest fires at 3 μm.Mathematical calculations showed that the physical properties of the sensilla are such that the expected temperature rise is insufficient for transduction of the infrared signal through mechanical means or as a thermal receptor as previously thought;hence the protein plays the pivotal role in perception of single photons and transmission of the signal within the sensilla.     

The Synthesis of Vicinal Diacylamines via Bamberger Ring Cleavage of Substituted Imidazoles. 1,2,4-Triacylaminobutanes Bearing Different Acyl Groups

Bamberger ring cleavage acylation of N-p-toluenesulfonyl (tosyl) histamine followed by hydrogenation yields 3,4-diacylaminobutanetosylamine, a triamine containing groups with different chemical reactivities.     

Lower bounds for multivariate approximation by affine-invariantdictionaries

The problem of approximating locally smooth multivariate functions by linear combinations of elements from an affine-invariant redundant dictionary is considered. Augmenting previous upper bound results for approximation, we establish lower bounds on the performance of such schemes. The lower bounds are tight to within a logarithmic factor in the number of elements used in the approximation. Using a previously introduced notion of nonlinear approximation, we show that the approximation ability may be completely characterized by the pseudodimension of the approximation space with respect to a finite set of points. This result establishes a useful link between the problems of approximation and estimation, or learning, the latter often being conveniently characterized, at least in terms of upper bounds, by the pseudodimension     

A Comparative Study of Continuous and Periodic Inspection Policies in Deteriorating Production Systems

Continuous or periodic inspection policies are often used to monitor the maintenance of a production process. In this paper, a comparative study of continuous and periodic inspection policies in deteriorating production systems is provided. The overall cost function is comprised of: set-up, inspection, inventory holding, defective and restoration costs. Different models, constant, linear and exponential, of restoration costs are considered. Tradeoffs between the continuous and the periodic inspection policies are analyzed, and ranges of values of system or cost parameters for which the continuous inspection policy is preferred are established.