An analysis of segregation-induced changes in grain boundary cohesion in bcc iron

We thoroughly compare available experimental as well as theoretical values of the strengthening/embrittling energy of numerous solutes at grain boundaries in α-iron and assess their reliability. The strengthening/embrittling energy is displayed according to its relationship to the difference of corresponding sublimation enthalpies of the host and the solute as well as with regard to the position of the solute in the Periodic Table.     

Behaviour of an optically trapped probe approaching a dielectric interface

Abstract

The way in which reflection of the trapping beam from a dielectric interface influences the distance of the trapped sphere from the beam waist is studied theoretically and experimentally. The reflected wave interferes with the incident wave and they create a standing-wave component in the total axial intensity distribution. This component then modulates the trapping potential and creates several possible equilibrium positions for the trapped sphere. When the beam waist approaches the surface, the potential profile changes, which consequently causes jumps of the trapped probe from its current location to a deeper potential well. We suggested theoretically and proved experimentally that the magnitude of these unwanted jumps between the neighbouring equilibrium positions can be decreased by a suitable size of the sphere.     

On decidability of LTL model checking for process rewrite systems

We establish a decidability boundary of the model checking problem for infinite-state systems defined by Process Rewrite Systems (PRS) or weakly extended Process Rewrite Systems (wPRS), and properties described by basic fragments of action-based Linear Temporal Logic (LTL) with both future and past operators. It is known that the problem for general LTL properties is decidable for Petri nets and for pushdown processes, while it is undecidable for PA processes.We show that the problem is decidable for wPRS if we consider properties defined by LTL formulae with only modalities strict eventually, strict always, and their past counterparts. Moreover, we show that the problem remains undecidable for PA processes even with respect to the LTL fragment with the only modality until or the fragment with modalities next and infinitely often.     

Depth profile analyses of nc-TiC/a-C:H coating prepared by balanced magnetron sputtering

Nanocrystalline titanium carbide embedded in an hydrogenated amorphous carbon matrix (nc-TiC/a-C:H) shows high hardness and Young's modulus together with low wear and low friction coefficient. In this paper, we report on the preparation of well adherent nc-TiC/a-C:H coatings ~ 5 μm thick on stainless steel substrates using a well balanced magnetic field configuration and only very low power RF bias on the substrate. Hardness and Young's modulus of these coatings are 43 GPa and 380 GPa, respectively. The mechanical properties – hardness and Young's modulus – measured from the coating's top reach the values obtained at optimized experiments where the unbalanced magnetic field configuration was used. A simple method of depth profiling suitable for evaluation of mechanical properties of several micrometers thick coatings is developed and employed. The paper reports on the depth profile analyses of the coating hardness, Young's modulus, composition and morphology.     

Fingerprinting Biogenic Aldehydes through Pattern Recognition Analyses of Excitation–Emission Matrices

Biogenic carbonyls, especially aldehydes, have previously demonstrated their potential to serve as early diagnostic biomarkers for disease and injury that have not been fully realized owing, in part, to the lack of a rapid and simple point-of-care method for aldehyde identification. The ability to determine which carbonyl compound is elevated and not just the total aldehydic load may provide more disease- or injury-specific diagnostic information. Toward this end, a novel fluorophore is presented that is able to form a complex with biogenic carbonyls under catalyst-free conditions so as to give a fluorescent fingerprint of the resulting hydrazone. The successful identification of bound carbonyls was accomplished with a newly described algorithm that applied principal curvature analysis of excitation–emission matrices to reduce surface features to ellipse representations, followed by a pattern-matching routine. With this algorithm, carbonyls were identified over a range of concentrations, and mixture components were successfully parsed. Overall, the results presented lay the groundwork for novel implementations of chemometrics to low-cost, rapid, and simple-to-implement point-of-care diagnostics.     

"Multicolor" electrochemical labeling of DNA hybridization probes with osmium tetroxide complexes

Labeling of oligonucleotide reporter probes (RP) with electroactive markers has frequently been utilized in electrochemical detection of DNA hybridization. Osmium tetroxide complexes with tertiary amines (Os,L) bind covalently to pyrimidine (predominantly thymine) bases in DNA, forming stable, electrochemically active adducts. We propose a technique of electrochemical "multicolor" DNA coding based on RP labeling with Os,L markers involving different nitrogenous ligands (such as 2,2' -bipyridine, 1,10-phenanthroline derivatives or N,N,N',N'-tetramethylethylenediamine). At carbon electrodes the Os,L-labeled RPs produce specific signals, with the potentials of these differing depending on the ligand type. When using Os,L markers providing sufficiently large differences in their peak potentials, parallel analysis of multiple target DNA sequences can easily be performed via DNA hybridization at magnetic beads followed by voltammetric detection at carbon electrodes. Os,L labeling of oligonucleotide probes comprising a segment complementary to target DNA and an oligo(T) tail (to be modified with the osmium complex) does not require any organic chemistry facilities and can be achieved in any molecular biological laboratory. We also for the first time show that this technology can be used for labeling of oligonucleotide probes hybridizing with target DNAs that contain both purine and pyrimidine bases.     

Vicinal Diol-Tethered Nucleobases as Targets for DNA Redox Labeling with Osmate Complexes

Six-valent osmium (osmate) complexes with nitrogenous ligands have previously been used for the modification and redox labeling of biomolecules involving vicinal diol moieties (typically, saccharides or RNA). In this work, aliphatic (3,4-dihydroxybutyl and 3,4-dihydroxybut-1-ynyl) or cyclic (6-oxo-6-(cis-3,4-dihydroxypyrrolidin-1-yl)hex-2-yn-1-yl, PDI) vicinal diols are attached to nucleobases to functionalize DNA for subsequent redox labeling with osmium(VI) complexes. The diol-linked 2′-deoxyribonucleoside triphosphates were used for the polymerase synthesis of diol-linked DNA, which, upon treatment with K₂OsO₃ and bidentate nitrogen ligands, gave the desired Os-labeled DNA, which were characterized by means of the gel-shift assay and ESI-MS. Through ex situ square-wave voltammetry at a basal plane pyrolytic graphite electrode, the efficiency of modification/labeling of individual diols was evaluated. The results show that the cyclic cis-diol (PDI) was a better target for osmylation than that of the flexible aliphatic ones (alkyl- or alkynyl-linked). The osmate adduct-specific voltammetric signal obtained for Os^VI-treated DNA decorated with PDI showed good proportionality to the number of PDI per DNA molecule. The Os^VI reagents (unlike OsO₄) do not attack nucleobases; thus offering specificity of modification on the introduced glycol targets.