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In CHO cells transfected with the rat dopamine D2 receptor (long isoform), administration of dopamine per se elicited a concentration-dependent increase in arachidonic acid (AA) release. The maximal effect was 197% of controls (EC50=25 nM). The partial D2 receptor agonist, (-)-(3-hydroxyphenyl)-N-n-propylpiperidine [(-)-3-PPP], also induced AA release, but with somewhat lower efficacy (maximal effect: 165%; EC50=91 nM). The AA-releasing effect of dopamine was counteracted by pertussis toxin, by the inhibitor of intracellular Ca2+ release, 8-(N N-diethylamino)octyl-3,4,5-trimethoxybenzoate (TMB-8), by excluding calcium from the medium, by the phospholipase A2 (PLA2) inhibitor, quinacrine, and by long-term pretreatment with the phorbol ester, 12-O-tetradecanoylphorbol-13-acetate (TPA). In addition, it was antagonized by the D2 antagonists, raclopride and (-)-sulpiride--but not by (+)-sulpiride--and absent in sham-transfected CHO cells devoid of D2 receptors. The results obtained contrast to the previous notion that dopamine and other D2 receptor agonists require the concomitant administration of calcium-mobilizing agents such as ATP, ionophore A-23187 (calcimycin), thrombin, and TRH, to influence AA release from various cell lines. 相似文献
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The purpose of this investigation was to study the origin of the differences in paper‐to‐paper friction of linerboards based on old corrugated containers (OCC). The sheets were subjected to two extraction stages and analyzed with respect to, surface roughness, and their content of low‐molecular‐mass lipophilic compounds (LLC). Friction was measured using a friction tester based on the horizontal plane principle. The surface roughness was measured using a Perthometer profiler and the low molecular mass lipophilic constituent of the paper sheets was determined by gas chromatography‐mass spectroscopy. The sheets were imaged using environmental scanning electron microscopy (ESEM), and the relative compositions of inorganic ions on the paper surfaces were determined by energy dispersive X‐ray spectroscopy (EDS). The results showed that a high amount of LLC in the sheets lead to low friction, due to lubrication. It was also observed that large CaCO3 particles on the surface had a friction‐increasing effect, and that there was no relationship between the surface roughness and the friction. © 2002 Wiley Periodicals, Inc. J Appl Polym Sci 85: 1511–1520, 2002 相似文献
5.
This paper presents a new perspective on the stability problem for uncertain LTI feedback systems with actuator input amplitude saturation. The solution is obtained using the quantitative feedback theory and a 3 DoF non‐interfering control structure. Describing function (DF) analysis is used as a criterion for closed loop stability and limit cycle avoidance, but the circle or Popov criteria could also be employed. The novelty is the combination of a controller parameterization from the literature and describing function‐based limit cycle avoidance with margins for uncertain plants. Two examples are given. The first is a benchmark problem and a comparison is made with other proposed solutions. The second is an example that was implemented and tested on an X‐Y linear stage used for nano‐positioning applications. Design and implementation considerations are given. An example is given on how the method can be extended to amplitude and rate saturation with the help of the generalized describing function, and a novel anti‐windup compensation structure inspired by previous contributions. Copyright © 2015 John Wiley & Sons, Ltd. 相似文献
6.
We have implemented a compiler for key parts of Modelica, an object-oriented language supporting equation-based modeling and simulation of complex physical systems. The compiler is extensible, to support experiments with emerging tools for physical models. To achieve extensibility, the implementation is done declaratively in JastAdd, a metacompilation system supporting modern attribute grammar mechanisms such as reference attributes and nonterminal attributes.This paper reports on experiences from this implementation. For name and type analyses, we illustrate how declarative design strategies, originally developed for a Java compiler, could be reused to support Modelica’s advanced features of multiple inheritance and structural subtyping. Furthermore, we present new general design strategies for declarative generation of target ASTs from source ASTs. We illustrate how these strategies are used to resolve a generics-like feature of Modelica called modifications, and to support flattening, a fundamental part of Modelica compilation. To validate that the approach is practical, we have compared the execution speed of our compiler to two existing Modelica compilers. 相似文献
7.
Johan kesson Torbjrn Ekman Grel Hedin 《Electronic Notes in Theoretical Computer Science》2008,203(2):117
This paper describes experiences from implementing key parts of a compiler for Modelica, an object-oriented language supporting declarative modeling and simulation of complex physical systems. Our implementation uses the attribute-grammar based tool JastAdd. In particular, we discuss the implementation of Modelica name analysis which is highly context-dependent, type analysis which is based on structural subtyping, and flattening which is a fundamental part of the Modelica compilation process.of so called modifications, Modelica. 相似文献
8.
Lundgren U. Ekman J. Delsing J. 《Electromagnetic Compatibility, IEEE Transactions on》2006,48(4):766-773
Ten different commercially available conductive thermoplastic materials have been tested for near- and far-field shielding effectiveness (SE). Far-field SE was tested using a modified standard measurement technique to provide results comparable with the company-provided data. Further, housings of different thermoplastic materials were constructed and equipped with an electromagnetic interference (EMI) source to model a realistic near-field SE situation. The SE data up to 1 GHz is presented. Conductive thermoplastic materials with fillings of stainless steel fibers and nickel-coated carbon fibers were the two materials that offer the best far-field shielding performance. For the near-field shielding, two materials with filling of stainless steel fibers were the best performing ones. A thermoplastic with polycarbonate (PC) base and stainless steel content of 1.5 vol% showed the best combined far- and near-field shielding results 相似文献
9.
Jing Li Olof B. Widlund 《International journal for numerical methods in engineering》2006,66(2):250-271
The FETI‐DP and BDDC algorithms are reformulated using Block Cholesky factorizations, an approach which can provide a useful framework for the design of domain decomposition algorithms for solving symmetric positive definite linear system of equations. Instead of introducing Lagrange multipliers to enforce the coarse level, primal continuity constraints in these algorithms, a change of variables is used such that each primal constraint corresponds to an explicit degree of freedom. With the new formulation of these algorithms, a simplified proof is provided that the spectra of a pair of FETI‐DP and BDDC algorithms, with the same set of primal constraints, are essentially the same. Numerical experiments for a two‐dimensional Laplace's equation also confirm this result. Copyright © 2005 John Wiley & Sons, Ltd. 相似文献
10.
Embedded control devices today usually allow parameter changes, and possibly activation of different pre-implemented algorithms. Full reprogramming using the complete source code is not allowed for safety, efficiency, and proprietary reasons. For these reasons, embedded regulators are quite rigid and closed concerning the control structure. In several applications, like industrial robots, there is a need to tailor the low level control to meet specific application demands. In order to meet the efficiency and safety demands, a way of building more generic and open regulators has been developed. The key idea is to use pieces of compiled executable code as functional operators, which in the simplest case may appear as ordinary control parameters. In an object oriented framework, this means that new methods can be added to controller objects after implementation of the basic control, even at run-time. The implementation was carried out in industrially well accepted languages such as C and C++. The dynamic binding at run-time differs from ordinary dynamic linking in that only a subset of the symbols can be used. This subset is defined by the fixed part of the system. The safety demands can therefore still be fulfilled. Encouraged by results from fully implemented test cases, we believe that extensive use of this concept will admit more open, still efficient, embedded systems. 相似文献