全文获取类型
收费全文 | 9063篇 |
免费 | 865篇 |
国内免费 | 36篇 |
专业分类
电工技术 | 133篇 |
综合类 | 13篇 |
化学工业 | 1929篇 |
金属工艺 | 244篇 |
机械仪表 | 391篇 |
建筑科学 | 242篇 |
矿业工程 | 4篇 |
能源动力 | 400篇 |
轻工业 | 745篇 |
水利工程 | 31篇 |
石油天然气 | 4篇 |
无线电 | 1765篇 |
一般工业技术 | 1992篇 |
冶金工业 | 811篇 |
原子能技术 | 126篇 |
自动化技术 | 1134篇 |
出版年
2023年 | 84篇 |
2022年 | 53篇 |
2021年 | 207篇 |
2020年 | 193篇 |
2019年 | 233篇 |
2018年 | 313篇 |
2017年 | 253篇 |
2016年 | 368篇 |
2015年 | 311篇 |
2014年 | 414篇 |
2013年 | 671篇 |
2012年 | 511篇 |
2011年 | 664篇 |
2010年 | 519篇 |
2009年 | 529篇 |
2008年 | 516篇 |
2007年 | 423篇 |
2006年 | 378篇 |
2005年 | 317篇 |
2004年 | 305篇 |
2003年 | 266篇 |
2002年 | 243篇 |
2001年 | 209篇 |
2000年 | 173篇 |
1999年 | 173篇 |
1998年 | 305篇 |
1997年 | 225篇 |
1996年 | 169篇 |
1995年 | 131篇 |
1994年 | 116篇 |
1993年 | 79篇 |
1992年 | 58篇 |
1991年 | 61篇 |
1990年 | 62篇 |
1989年 | 53篇 |
1988年 | 39篇 |
1987年 | 45篇 |
1986年 | 50篇 |
1985年 | 32篇 |
1984年 | 26篇 |
1983年 | 31篇 |
1982年 | 7篇 |
1981年 | 20篇 |
1980年 | 20篇 |
1979年 | 10篇 |
1978年 | 14篇 |
1977年 | 18篇 |
1976年 | 25篇 |
1975年 | 10篇 |
1972年 | 5篇 |
排序方式: 共有9964条查询结果,搜索用时 15 毫秒
1.
So Young Kim Cheol Park Min Yeong Kim Seon Yeong Ji Hyun Hwangbo Hyesook Lee Su Hyun Hong Min Ho Han Jin-Woo Jeong Gi-Young Kim Chang-Gue Son JaeHun Cheong Yung Hyun Choi 《International journal of molecular sciences》2021,22(9)
Coptidis Rhizoma is the dried rhizome from the Coptis chinensis Franch. that has been shown to have a number of beneficial pharmacological properties including antioxidant, anti-inflammatory, and anti-cancer effects. However, the anti-cancer effects of Coptidis Rhizoma on hepatocellular carcinoma (HCC) remain unclear. In this study, we investigated the anti-cancer properties of Coptidis Rhizoma ethanol extract (CR) in HCC Hep3B cells and in a xenograft mouse model. Our results showed that the CR significantly inhibited cell growth and induced apoptosis in Hep3B cells through increased expression of Bcl-2 associated x-protein (Bax) and cleavage of poly-ADP ribose polymerase (PARP), reduced expression of Bcl-2, and activated caspases. CR also increased the generation of intracellular reactive oxygen species (ROS), which caused a loss of mitochondrial membrane potential (MMP, ΔΨm) and activation of the mitochondria-mediated intrinsic apoptosis pathway. Moreover, N-acetylcysteine (NAC), a ROS inhibitor, markedly blocked the effects of CR on apoptotic pathways. CR also induced the expression of light chain 3 (LC3)-I/II, a key autophagy regulator, whereas CR-mediated autophagy was significantly suppressed by NAC. In addition, pre-treatment with NAC perfectly attenuated the inhibition of cell invasion and migration of CR-stimulated Hep3B cells. Furthermore, oral administration of CR suppressed Hep3B tumor growth in xenograft mice without toxicity, alterations to body weight, or changes in hematological and biochemical profiles. Taken together, our findings suggest that CR has anti-tumor effects that result from ROS generation, and may be a potential pharmacological intervention for HCC. 相似文献
2.
Made Angga Akwiditya Chean Yeah Yong Mohd Termizi Yusof Abdul Razak Mariatulqabtiah Kok Lian Ho Wen Siang Tan 《International journal of molecular sciences》2021,22(5)
Gene therapy research has advanced to clinical trials, but it is hampered by unstable nucleic acids packaged inside carriers and there is a lack of specificity towards targeted sites in the body. This study aims to address gene therapy limitations by encapsidating a plasmid synthesizing a short hairpin RNA (shRNA) that targets the anti-apoptotic Bcl-2 gene using truncated hepatitis B core antigen (tHBcAg) virus-like particle (VLP). A shRNA sequence targeting anti-apoptotic Bcl-2 was synthesized and cloned into the pSilencer 2.0-U6 vector. The recombinant plasmid, namely PshRNA, was encapsidated inside tHBcAg VLP and conjugated with folic acid (FA) to produce FA-tHBcAg-PshRNA VLP. Electron microscopy revealed that the FA-tHBcAg-PshRNA VLP has an icosahedral structure that is similar to the unmodified tHBcAg VLP. Delivery of FA-tHBcAg-PshRNA VLP into HeLa cells overexpressing the folate receptor significantly downregulated the expression of anti-apoptotic Bcl-2 at 48 and 72 h post-transfection. The 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay demonstrated that the cells’ viability was significantly reduced from 89.46% at 24 h to 64.52% and 60.63%, respectively, at 48 and 72 h post-transfection. As a conclusion, tHBcAg VLP can be used as a carrier for a receptor-mediated targeted delivery of a therapeutic plasmid encoding shRNA for gene silencing in cancer cells. 相似文献
3.
This article presents a design methodology for linearizing GaN HEMT amplifiers based on splitting a large FET into multiple parallel FETs with same total gate periphery and by biasing them individually. By varying the biases, the magnitude and the phase of the IMD3 components at the output of FET changes. A detailed simulation methodology using commercial microwave CAD software is presented. Simulation results show that by biasing one device in Class AB and other(s) in deep Class AB mode, IMD3 components of parallel FETs can be made out of phase to each other leading to cancellation and improvement in linearity. Three prototype circuits were simulated using (a) a single 5 mm FET (1 × 5 mm), (b) two parallel 2.5 mm FETs (2 × 2.5 mm), and (c) four parallel 1.25 mm FETs (4 × 1.25 mm), for a total gate periphery of 5 mm, over the frequency range of 0.8 to 1.0 GHz. IMD3 improvement up to 20 dBc was achieved with the 4 × 1.25 mm circuit when the FET biases were optimized. Measurement results show improvement in linearity up to 20 dBc for 4 × 1.25 mm circuit. The proposed method improves linearity without a substantial penalty on the power consumption and is straightforward to implement. 相似文献
4.
5.
Kim Ho Jin Lee Mi Jin Park Hye Jin Kim Hye Jin Cho Soon Kil Jeong Min Hee 《Food science and biotechnology》2018,27(3):877-882
Food Science and Biotechnology - A new analytical method was developed for the simultaneous determination of seven food additives (Ponceau 4R, Allura Red AC, Amaranth, 4-hydroxymethyl benzoic acid,... 相似文献
6.
Molecular Ordering: Selectivity of Threefold Symmetry in Epitaxial Alignment of Liquid Crystal Molecules on Macroscale Single‐Crystal Graphene (Adv. Mater. 40/2018) 下载免费PDF全文
7.
Nuruk is a fermentation agent used to manufacture alcoholic beverages, which contains a variety of microorganisms. Most microorganisms in Nuruk are useful for the production of alcoholic beverages; however, Nuruk can be infected with Aspergillus flavus, which produces aflatoxin (AF). Therefore, this study analyzed total AF concentrations in Nuruk, the transfer of AF from Nuruk to alcoholic beverages, and AF-producing microorganisms to determine the safety of alcoholic beverages with respect to this toxin. ELISA showed that total AF levels in 14 of 61 Nuruk samples exceeded 15 ppb, the Korean permissible level in cereal products. In alcoholic beverages produced with Nuruk containing AF levels >15 ppb, only AF G1 was detected, at a level of 0.3 ppb, and the transfer ratio of AF G1 was approximately 1.2% to 1.3%. The dominant genera in Nuruk were Aspergillus and Rhizopus. Among 30 strains belonging to Aspergillus, 10 produced only AF B1 at levels of 0.1 to 2.4 ppb after incubation at 25 °C for 8 days on potato dextrose agar plates. Although AF in Nuruk was rarely transferred to alcoholic beverages and the aflatoxigenic strains were found to possess poor AF-producing capacity, further efforts to reduce AF in Nuruk are needed to ensure the safety of alcoholic beverages. 相似文献
8.
Scientometrics - Although scientists, like many other professionals, aspire to fame and recognition, research in the emergent field of fame and celebrity has as yet neglected to explore their fame... 相似文献
9.
Peida Zhao Ruixuan Wang Der‐Hsien Lien Yingbo Zhao Hyungjin Kim Joy Cho Geun Ho Ahn Ali Javey 《Advanced materials (Deerfield Beach, Fla.)》2019,31(48)
Scanning probe lithography is used to directly pattern monolayer transition metal dichalcogenides (TMDs) without the use of a sacrificial resist. Using an atomic‐force microscope, a negatively biased tip is brought close to the TMD surface. By inducing a water bridge between the tip and the TMD surface, controllable oxidation is achieved at the sub‐100 nm resolution. The oxidized flake is then submerged into water for selective oxide removal which leads to controllable patterning. In addition, by changing the oxidation time, thickness tunable patterning of multilayer TMDs is demonstrated. This resist‐less process results in exposed edges, overcoming a barrier in traditional resist‐based lithography and dry etch where polymeric byproduct layers are often formed at the edges. By patterning monolayers into geometric patterns of different dimensions and measuring the effective carrier lifetime, the non‐radiative recombination velocity due to edge defects is extracted. Using this patterning technique, it is shown that selenide TMDs exhibit lower edge recombination velocity as compared to sulfide TMDs. The utility of scanning probe lithography towards understanding material‐dependent edge recombination losses without significantly normalizing edge behaviors due to heavy defect generation, while allowing for eventual exploration of edge passivation schemes is highlighted, which is of profound interest for nanoscale electronics and optoelectronics. 相似文献
10.
Feifei Li Haoqiang Ai Changmin Shi Kin Ho Lo Hui Pan 《International Journal of Hydrogen Energy》2021,46(24):12886-12896
Electrochemical CO2 reduction reaction (CO2RR) is an efficient way in the utilization of CO2. In this work, single transition-metal (TM) atom (TM = Sc, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn) anchored on two-dimensional (2D) Ti2CN2 are designed for CO2RR using density-functional-theory (DFT) calculation. We show that Ti2CN2 serves as an excellent substrate to support single atom catalysts (SACs), compared to Ti2CO2 and Ti2CF2. We find that the Sc, Ti and V supported on Ti2CN2 show high catalytic activities to produce CO with a low overpotential of 0.37, 0.27, and 0.23 eV, respectively. Differently, the Mn and Fe on Ti2CN2 are catalytically active for the production of HCOOH with a low overpotential of 0.32 and 0.43 eV, respectively. We further show that the negatively charged TM-Ti2CN2 can capture and activate CO2 more effectively, and the catalytic activity and selectivity can be significantly tuned by injecting extra electrons. 相似文献