Pro-Resolving Lipid Mediator Resolvin E1 Mitigates the Progress of Diethylnitrosamine-Induced Liver Fibrosis in Sprague-Dawley Rats by Attenuating Fibrogenesis and Restricting Proliferation

Liver fibrosis is a complex process associated to most types of chronic liver disease, which is characterized by a disturbance of hepatic tissue architecture and the excessive accumulation of extracellular matrix. Resolvin E1 (RvE1) is a representative member of the eicosapentaenoic omega-3 lipid derivatives, and is a drug candidate of the growing family of endogenous resolvins. Considering the aforementioned, the main objective of this study was to analyze the hepatoprotective effect of RvE1 in a rat model of liver fibrosis. Male Sprague-Dawley rats received diethylnitrosamine (DEN, 70 mg/mg body weight intraperitoneally (i.p)) as an inductor of liver fibrosis once weekly and RvE1(100 ng/body weight i.p) twice weekly for four weeks. RvE1 suppressed the alterations induced by DEN, normalizing the levels of alanine aminotransferase (ALT), albumin, and lactate dehydrogenase (LDH), and ameliorated DEN injury by decreasing the architecture distortion, inflammatory infiltration, necrotic areas, and microsteatosis. RvE1 also limited DEN-induced proliferation through a decrease in Ki67-positive cells and cyclin D1 protein expression, which is related to an increase of the levels of cleaved caspase-3. Interestingly, we found that RvE1 promotes higher nuclear translocation of nuclear factor κB (NF-κB)p65 than DEN. RvE1 also increased the levels of nuclear the nuclear factor erythroid 2–related factor 2 (Nrf2), but with no antioxidant effect, measured as an increase in glutathione disulfide (GSSG) and a decrease in the ratio of glutathione (GSH)/GSSG. Taken together, these results suggest that RvE1 modulates the fibrogenesis, steatosis, and cell proliferation in a model of DEN induced fibrosis.     

The Role of Cholesterol on Triterpenoid Saponin-Induced Endolysosomal Escape of a Saporin-Based Immunotoxin

Cholesterol seems to play a central role in the augmentation of saporin-based immunotoxin (IT) cytotoxicity by triterpenoid saponins. Endolysosomal escape has been proposed as one mechanism for the saponin-mediated enhancement of targeted toxins. We investigated the effects of lipid depletion followed by repletion on Saponinum album (SA)-induced endolysosomal escape of Alexa Fluor labelled saporin and the saporin-based immunotoxin OKT10-SAP, directed against CD38, in Daudi lymphoma cells. Lipid deprived cells showed reduced SA-induced endolysosomal escape at two concentrations of SA, as determined by a flow cytometric method. The repletion of membrane cholesterol by low density lipoprotein (LDL) restored SA-induced endolysosomal escape at a concentration of 5 µg/mL SA but not at 1 µg/mL SA. When LDL was used to restore the cholesterol levels in lipid deprived cells, the SA augmentation of OKT10-SAP cytotoxicity was partially restored at 1 µg/mL SA and fully restored at 5 µg/mL SA. These results suggest that different mechanisms of action might be involved for the two different concentrations of SA and that endosomal escape may not be the main mechanism for the augmentation of saporin IT cytotoxicity by SA at the sub-lytic concentration of 1 µg/mL SA.     

TESLA: an extended study of an energy-saving agent that leverages schedule flexibility

This paper presents transformative energy-saving schedule-leveraging agent (TESLA), an agent for optimizing energy usage in commercial buildings. TESLA’s key insight is that adding flexibility to event/meeting schedules can lead to significant energy savings. This paper provides four key contributions: (i) online scheduling algorithms, which are at the heart of TESLA, to solve a stochastic mixed integer linear program for energy-efficient scheduling of incrementally/dynamically arriving meetings and events; (ii) an algorithm to effectively identify key meetings that lead to significant energy savings by adjusting their flexibility; (iii) an extensive analysis on energy savings achieved by TESLA; and (iv) surveys of real users which indicate that TESLA’s assumptions of user flexibility hold in practice. TESLA was evaluated on data gathered from over 110,000 meetings held at nine campus buildings during an 8-month period in 2011–2012 at the University of Southern California and Singapore Management University. These results and analysis show that, compared to the current systems, TESLA can substantially reduce overall energy consumption.     

Nutrient budget for Saguling Reservoir,West Java,Indonesia

A preliminary nutrient budget for Saguling Reservoir is reported as a first attempt to quantify the behaviour of nutrients entering this reservoir. This work is part of a larger Indonesia-Australia collaborative research and training project, involving Padjadjaran University and Monash University, established to study nutrient dynamics in Saguling Reservoir. Saguling Reservoir, the first of a chain of three large reservoirs (Saguling, Cirata and Jatilahur), built on the Citarum River in central Java, was completed in 1985. It has already become highly polluted, particularly with domestic and industrial effluent (organic matter, nutrients, heavy metals) from the urban areas of Bandung (population 2 million). The reservoir experiences major water quality problems, including excessive growths of floating plants, toxic cyanobacterial blooms and regular fish-kills. The work reported in this paper shows that Saguling receives a very large nutrient load from the city of Bandung and because of this, is highly eutrophic. It is unlikely that the water quality of Saguling will improve until a substantial part of Bandung is sewered and adequate discharge controls are placed on the many industries in the region upstream of the reservoir.     

A Maternal High Fat Diet Has Long‐Lasting Effects on Skeletal Muscle Lipid and PLIN Protein Content in Rat Offspring at Young Adulthood

A maternal high fat diet (HFD) can have adverse effects on skeletal muscle development. Skeletal muscle PLIN proteins (PLIN2, 3 and 5) are thought to play critical roles in lipid metabolism, however effects of HFD on PLIN and lipases (HSL, ATGL, CGI‐58) in mothers as well as their offspring have yet to be investigated. The primary objective of this study was to determine whether maternal HFD would influence skeletal muscle lipase and PLIN protein content in offspring at weaning (19d) and young adulthood (3mo). Female rats (28d old, n = 9/group) were fed control (CON, AIN93G, 7 % soybean oil) or HFD (AIN93G, 20 % lard) for 10 weeks prior to mating and throughout pregnancy and lactation. All offspring were weaned to CON [n = 18/group, 1 female and 1 male pup per litter were studied at weaning (19d) and 3mo of age]. There was no effect of sex for the main outcomes measured in plantaris, therefore male and female data was combined. Maternal HFD resulted in higher triacylglycerol content in pups at 3mo (p < 0.05), as well as in the dams (p = 0.015). Maternal HFD resulted in higher PLIN5 content in pups at weaning and 3mo (p = 0.05). PLIN2 and PLIN5 content decreased at 3mo versus weaning (p < 0.001). HFD dams had a higher PLIN3 content (p = 0.016). Diet had no effect on ATGL, CGI‐58, or HSL content. In conclusion, exposure to a maternal HFD resulted in higher skeletal muscle lipid and PLIN5 content in plantaris of offspring through to young adulthood.     

Developing New Multivariate Process Capability Indices for Autocorrelated Data

Traditionally, the process capability index is developed assuming that the process output data are independent and follow normal distribution. However, in most environmental cases, the process data have more than one quality characteristic and exhibit property of autocorrelation. We propose two novel multivariate process capability indices for autocorrelated data, NMAC_p and NMAC_pm, for the nominal‐the‐best case. For the smaller‐the‐better case, Γ(0) is used to modify the ND index and a new multivariate autocorrelated process capability index NMAC_pu is derived. Furthermore, a simulation study is conducted to compare the performance of the various multivariate autocorrelated indices. The simulation results show that the actual nonconforming rates can be correctly reflected by our proposed indices, which outperform the previous C_p^m, MC_p, MC_pm, NMC_p, NMC_pm, and ND indices under different time series models. Thus, our proposed capability indices can be used in evaluating the performance of multivariate autocorrelated processes. Finally, a realistic example in hydrological application further demonstrates the usefulness of our proposed capability indices. Copyright © 2013 John Wiley & Sons, Ltd.     

Alice,Finnegan&Oliver 还记得没它们之前日子是什么样吗

正加拿大摄影师Holly SissonMonica Sisson这是一个让人"质壁分离"的家庭,两猫一狗,主人都是摄影师,住在加拿大的多伦多市。在她们的照片中,生活平淡可爱又满满当当,我们无法想象,如果这些画面里没有它们,会是什么样子。     

Max Penson in Uzbekistan

Photography reached the Ottoman Empire soon after its invention, at about the time that painting began to be practised there as part of a broader project of assimilating aspects of European culture. This was in marked contrast to the situation in Europe, where photography had to contend with visual traditions from which it adopted pictorial conventions and subject matter. Instead of supplanting existing traditions of realistic visual representation, photography in the Ottoman Empire served as a discrete source of inspiration. This paper examines how this inversion of European experience within the Ottoman Empire provides an alternative to dominant narratives of photographic history. If one of the salient characteristics of modernist movements in Western art is their ability to break with tradition, then the adoption of Western practices of representation by artists within the Ottoman Empire may be viewed as a radical modernist success.