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The accurate knowledge of the elastic properties of single-stranded DNA (ssDNA) is key to characterize the thermodynamics of molecular reactions that are studied by force spectroscopy methods where DNA is mechanically unfolded. Examples range from DNA hybridization, DNA ligand binding, DNA unwinding by helicases, etc. To date, ssDNA elasticity has been studied with different methods in molecules of varying sequence and contour length. A dispersion of results has been reported and the value of the persistence length has been found to be larger for shorter ssDNA molecules. We carried out pulling experiments with optical tweezers to characterize the elastic response of ssDNA over three orders of magnitude in length (60–14 k bases). By fitting the force-extension curves (FECs) to the Worm-Like Chain model we confirmed the above trend:the persistence length nearly doubles for the shortest molecule (60 b) with respect to the longest one (14 kb). We demonstrate that the observed trend is due to the different force regimes fitted for long and short molecules, which translates into two distinct elastic regimes at low and high forces. We interpret this behavior in terms of a force-induced sugar pucker conformational transition (C3′-endo to C2′-endo) upon pulling ssDNA.  相似文献   
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The noninvasive sampling of dermal interstitial fluid (ISF) for the monitoring of clinical biomarkers is a greatly appealing area of research. The identification of molecular biomarkers in biological fluids has been accelerated with -omics analyses but remains limited in ISF because of its time-consuming and complex extraction process. Here, the generation of microneedle (MN) patches made of superabsorbent acrylate-based hydrogels for the rapid sampling of dermal ISF is described to explore its proteome. In depth, iterative optimization allows the identification of novel acrylate-based compositions with the required chemical, mechanical, and biocompatibility properties allowing proteomic analysis of the extracted ISF for the first time after sampling with swelling MNs. The generated MN arrays show no cytotoxic effect, successfully cross the stratum corneum, and can collect up to 6 µL of dermal ISF in 10 min in vivo. Proteomics lead to the detection of 176 clinically relevant biomarkers in the collected samples validating the use of ISF as a relevant bodily fluid for disease monitoring and diagnostic. Importantly, it is discovered that extraction fingerprint is strongly dependent on the MNs chemistry, and thus specific biomarkers could be selectively extracted by tuning the composition of the patch, making the system versatile and specific.  相似文献   
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ABSTRACT

People around the world spend hours of their daily lives using smartphones; therefore, it is important to conduct cross-cultural research on the effects of smartphone use on health and well-being as culture influences values, motivations and communication patterns. The purpose of this study was to explore 5 popular uses of the smartphone – messaging, browsing the Internet, posting social content, reading social content, and playing games – how they relate to anxiety and depression scores, and how they vary depending on the country of the participants: Spain, the United States, and Colombia. In all three countries the ranking of most popular uses was the same: (1) Messaging, (2) Reading social content, and (3) Browsing the Internet. In the USA, game playing contributed to anxiety scores whereas reading social content was a protective factor; regarding depression scores, text messaging was a contributing factor. In Spain, browsing the Internet contributed to anxiety scores; regarding depression scores, messaging was a contributing factor and posting social content was a protective factor. In Colombia, no specific use influenced anxiety scores; regarding depression scores, only game playing was a protective factor. Our results showed that in all the countries, problematic smartphone use contributed to anxiety scores.  相似文献   
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The high quality of electrical power in high power and high reliability applications is a crucial necessity even under fault mode function. However, in these conditions, the quality of the torque is a key feature. To overcome this problem, the multiphase permanent magnet (PM) motors seems to be a very attractive choice. In order to highlight the robustness and reliability of this technology, this paper investigates the control of a five-phase PM motor under an open circuited phase fault conditions. Moreover, a High Order Sliding Mode (HOSM) controller combined to an optimal reference current generation is tested and compared to a PID controller under fault mode conditions. This original control strategy is proposed for faulted conditions. Compared to classical fault tolerant control, this strategy allows a better dynamic tracking of the non-sinusoidal reference currents and leads to a smooth torque with minimal losses even in severe fault conditions. To validate the proposed control strategy, simulation, and experimental results are presented and discussed.  相似文献   
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Sensors for monitoring temperature, heat flux, and thermal radiation are essential for applications such as electronic skin. While pyroelectric and thermoelectric effects are suitable candidates as functional elements in such devices, both concepts show individual drawbacks in terms of zero equilibrium signals for pyroelectric materials and small or slow response of thermoelectric materials. Here, these drawbacks are overcome by introducing the concept of thermodiffusion‐assisted pyroelectrics, which combines and enhances the performance of pyroelectric and ionic thermoelectric materials. The presented integrated concept provides both rapid initial response upon heating and stable synergistically enhanced signals upon prolonged exposure to heat stimuli. Likewise, incorporation of plasmonic metasurfaces enables the concept to provide both rapid and stable signals for radiation‐induced heating. The performance of the concept and its working mechanism can be explained by ion–electron interactions at the interface between the pyroelectric and ionic thermoelectric materials.  相似文献   
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