Hydroxyapatite cages with aligned pores for bone grafting – Seeding of human osteoblast-like cells in vitro and their response in dynamic culture mode

Hydroxyapatite (HA) is a highly regarded synthetic bone graft material. Porous HA ceramics blocks are used to substitute harvested natural bone grafts. Being similar to bone mineral, HA material integrates with the host bone through surface osteointegration and slowly resorb along with the natural bone remodeling process. The blocks in use currently have random and tortuous pore structures. The present work explores the usefulness of cage-like HA ceramic design with end-to-end open pores, with the help of in vitro cell culture methods. Such a structure, on implantation, will take up the blood factors and cells and host the bone remodeling process inside the bulk of the cage, leading to early healing. In the study, HA samples with aligned through-pores were prepared and explored in vitro, with a focus on how the pores host the cells inside and to what level the cells maintain their activity. Human osteoblast-like cells (HOS) were used, at different seeding and culturing approaches. Cell seeding was done through (i) conventional large volume cell suspension, (ii) a confined mini chamber with a limited volume of cell suspension, and (iii) placing a concentrated drop of cell suspension directly on top of the scaffold. The third approach gave the best cell adhesion and proliferation, and hence used for further explorations. A dynamic culture system was designed in-house by bifurcating the cell culture wells using vertical inserts, holding the samples horizontally with their ends open to both sides, and making the media flow across using a rocker platform. The HOS cell adhesion, viability and proliferation were tested in the HA cages, in static and dynamic culture conditions, with conventional porous ceramics as the control. The cell infiltration was deeper and the cell viability over a period of 7 days was significantly higher in dynamic culture conditions in the test samples.     

岩石圈深部温度压力条件下褐煤与水的热模拟研究

在压力高达1—3GPa、温度为400—700℃的条件下，在密闭体系中进行了褐煤加水的模拟实验。分析了实验产物中液态烃的变化规律，并讨论了压力、温度及恒温时间对有机质演化的影响。实验结果表明，热模拟液态产物氯仿沥青“A”的有机碳含量为0．91％-2．55％，2GPa条件下其高峰值后移至700℃，说明高压抑制了液态烃的生成，同时压力升高有利于有机质降解产物的环化、聚合和芳构化。在400—600℃条件下，温度升高或恒温时间增长，OEP和Pr／Ph值均减小；而在700℃的恒温条件下，压力增高，OEP和Pr／Ph值均增大。说明有机质的成熟度与温度和加热时间成正相关，而压力增加抑制了有机质的成熟演化。在高压条件下，芳烃演化的主要趋势是甲基化作用，压力升高有利于甲基化反应和甲基重排。     

应用金属迭片挠性联轴器改造D1000-32型风机

使用液力偶合器因故障频繁，对高炉稳产、高产造成严重威胁，而且维修费用较高，对偶合器改造后，应用金属迭片挠性联轴器其故障率、维修费用都很低。     

利用计算机进行油气资源评价和勘探决策

本文着重介绍了构成《现代勘探决策理论系统》的5个主要计算机模块,即:生油岩热演化处理、求任意曲边形面积、数学模拟预测资源量、勘探经济评价分析及决策、辅助处理等模块。该系统曾在塔里木盆地和准噶尔盆地的油气资源评价和勘探决策中发挥了作用,受到了专家和用户的好评。     

Proteomic Analysis of Embryogenesis and the Acquisition of Seed Dormancy in Norway Maple (Acer platanoides L.)

The proteome of zygotic embryos of Acer platanoides L. was analyzed via high-resolution 2D-SDS-PAGE and MS/MS in order to: (1) identify significant physiological processes associated with embryo development; and (2) identify changes in the proteome of the embryo associated with the acquisition of seed dormancy. Seventeen spots were identified as associated with morphogenesis at 10 to 13 weeks after flowering (WAF). Thirty-three spots were associated with maturation of the embryo at 14 to 22 WAF. The greatest changes in protein abundance occurred at 22 WAF, when seeds become fully mature. Overall, the stage of morphogenesis was characterized by changes in the abundance of proteins (tubulins and actin) associated with the growth and development of the embryo. Enzymes related to energy supply were especially elevated, most likely due to the energy demand associated with rapid growth and cell division. The stage of maturation is crucial to the establishment of seed dormancy and is associated with a higher abundance of proteins involved in genetic information processing, energy and carbon metabolism and cellular and antioxidant processes. Results indicated that a glycine-rich RNA-binding protein and proteasome proteins may be directly involved in dormancy acquisition control, and future studies are warranted to verify this association.     

Functional maturation during bovine granulopoiesis

Granulocytic precursor cells undergo morphologic changes in the nucleus and the cytoplasm during the process of granulopoiesis, which takes place in the bone marrow. These changes are associated with the development of stage-specific proteins necessary for the highly specialized roles of polymorphonuclear leukocytes in phagocytosis, bacterial killing, and in mediating the inflammatory process. The objective of the current study was to sequence the various events that occur upon functional development of granulocytic bone marrow cells in the bovine species. Cells were obtained from the bone marrow of clinically healthy cows and separated into different stages of maturation using density gradient centrifugation. Three cellular fractions were obtained that were enriched for either early immature, late immature or mature granulocytic cells. Functions and receptor expressions assessed in the three maturation stages were:Fc-IgG2 receptor and CD11b expression, phagocytosis of Escherichia coli, respiratory burst activity, and cellular myeloperoxidase activity. Immature cells expressed already Fc-IgG2 receptor and CD11b on their cytoplasma membrane. Phagocytic ability was acquired in the myelocytic stage, but only the more mature forms were readily capable of phagocytosis. Promyelocytes, myelocytes and metamyelocytes showed no respiratory burst activity. Only band and segmented cells produced reactive oxygen species. Myeloperoxidase was present at all stages of maturity. Thus, each of the maturation stages was characterized by a selective expression of one or more functions and receptors. Therefore, sequential biochemical maturation is postulated during bovine granulopoiesis.     

煤岩及泥炭成熟度热模拟研究--镜质组反射率演化特征及其地质意义

通过高温高压模拟实验，在20℃／h和2℃／h两种升温速率条件下，对沁水盆地山西组和太原组煤岩以及现代泥炭模拟固体产物镜质组反射率Ro演化特征进行了分析和研究．结果表明：1)在336．8～600℃的模拟温度区间，2℃／h和20℃／h两种升温速率条件下，模拟固体产物Ro值太原组煤岩分别为1．07％～4．50％和0．83％～3．97％，山西组煤岩分别为0．98％～4．51％和0．80％～4．05％，泥炭分别为0．96％～4．19％和0．72％～3．72％．2)随着实验温度的增高，煤岩以及泥炭的热模拟固体产物累积Ro值都呈直线增加，并且在实验温度区间范围内，2C／h升温速率条件下的Ro值增长值都要高于20℃／h升温速率条件下的Ro值增长值，说明升温速率是影响有机质热成熟演化过程的重要因素．3)在2种升温速率条件下，煤岩以及泥炭固体产物的Ro值随温度的相对波动较为明显，这主要反映了Ro值热演化的阶段性特征，总体来说，煤岩与泥炭的固体产物镜质组反射率在演化阶段上具有较多的相似性．但是太原组煤岩固体产物的Ro值变化相对幅度较大，而山西组煤岩和泥炭则较小．4)在相同的升温速率条件下，同一温度不同样品之间模拟固体产物的累积Ro值不同，大部分温度点处太原组煤岩Ro值最高，山西组煤岩次之，泥炭最低，并且各个样品之间Ro值的相对变化显，反映了样品性质对模拟固体产物Ro值演化的影响．结合实验所获得的结果和认识，对其地质意义进行了探讨．     

Internal chemical communication within flatworms

An understanding of reproductive function is important for control of parasitic helminths. In cestodes and trematodes virtually nothing is known about regulatory and coordinating mechanisms that control maturation, gamete formation, egg production, and related processes. Neurosecretory neurons have been reported in various species but specific modes of action of neurohormones have yet to be demonstrated. The role of ecdysone is being investigated.