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Diversified Structural Basis of a Conserved Molecular Mechanism for pH‐Dependent Dimerization in Spider Silk N‐Terminal Domains 下载免费PDF全文
Martins Otikovs Dr. Gefei Chen Kerstin Nordling Dr. Michael Landreh Prof. Dr. Qing Meng Dr. Hans Jörnvall Dr. Nina Kronqvist Dr. Anna Rising Prof. Dr. Jan Johansson Dr. Kristaps Jaudzems 《Chembiochem : a European journal of chemical biology》2015,16(12):1720-1724
Conversion of spider silk proteins from soluble dope to insoluble fibers involves pH‐dependent dimerization of the N‐terminal domain (NT). This conversion is tightly regulated to prevent premature precipitation and enable rapid silk formation at the end of the duct. Three glutamic acid residues that mediate this process in the NT from Euprosthenops australis major ampullate spidroin 1 are well conserved among spidroins. However, NTs of minor ampullate spidroins from several species, including Araneus ventricosus (AvMiSp NT), lack one of the glutamic acids. Here we investigate the pH‐dependent structural changes of AvMiSp NT, revealing that it uses the same mechanism but involves a non‐conserved glutamic acid residue instead. Homology modeling of the structures of other MiSp NTs suggests that these harbor different compensatory residues. This indicates that, despite sequence variations, the molecular mechanism underlying pH‐dependent dimerization of NT is conserved among different silk types. 相似文献
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Back Cover: Diversified Structural Basis of a Conserved Molecular Mechanism for pH‐Dependent Dimerization in Spider Silk N‐Terminal Domains (ChemBioChem 12/2015) 下载免费PDF全文
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Yuanzhang Jiang Cong Wang Shuai Zhang Lin Tan Jinlian Hu 《Advanced functional materials》2023,33(35):2303387
The exciting development of hydrogels makes it a promising candidate to be applied in various fields. However, it remains a great challenge to store the precursors of hydrogels and to process them after gelation, like synthetic polymer materials. Herein, spidroin-inspired novel nanogels with extraordinary processability, which can be spun into fibers via direct drawing and fabricated into thermal actuators easily after gelation are prepared. These soluble and spinnable nanogels are composed of a liquid metal core and a poly (acrylic acid) (PAA) shell and are entangled with each other. The as fabricated nanogels and diluted dope solution can be stored >1 month. The as-spun nanogel fibers with hierarchical structures achiev extraordinary mechanical properties (tensile stress of 575 MPa, toughness of 381 MJ m−3) and supercontraction at 60% RH. Besides, a photothermal actuator is prepared by coating the nanogels on a polyethylene substrate with a commercial shading ink, and the as-prepared actuator shows a rapid response to near-infrared light as well as a fast recovery. Molecular dynamics simulation reveals a possible working mechanism of the actuator. This study provides a new strategy to prepare processable nanogels with broad application prospects for smart textiles and soft robots. 相似文献
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