牙鲆淋巴囊肿病的PCR诊断方法研究

以中国养殖牙鲆 (Paralichthysolivaceus)淋巴囊肿病毒 (Lymphocystisdiseasevirus,LCDVcn)主要衣壳蛋白 (majorcapsidprotein ,MCP)基因的中间保守序列为目标基因 ,设计了一对特异性引物。该引物可扩增出 172bp的病毒DNA片段 ,其最小DNA检出量为 0 0 183ng。用PCR法从人工感染淋巴囊肿病毒 3天的牙鲆血、鳃、肝、脾、肠、胃及自然发病牙鲆的肿瘤中 ,分别检测到了LCDV的存在。本实验结果证明 ,PCR法对于早期检测LCDV是十分有效的。     

预防HIV传播的药物

药物预防HIV传播是人类战胜HIV的重要手段。现将预防HIV感染药的研发现状，抗HIV药在预防母婴传播，暴露前后预防的研发和应用动态作一综述。     

四株狂犬病街毒的免疫学特性

目的 研究国内分离的4株狂犬病街毒的免疫学特性。方法 采用免疫力试验及中和试验进行抗原性及免疫原性检测。结果4株街毒抗原性存在一定差异，应用aG株疫苗免疫小鼠能保护4株街毒的攻击。结论aG疫苗与4株街毒具有良好的交叉免疫性。     

构筑企业级信息系统安全体系

本文以跨地区公司为例,探讨企业级信息系统安全体系的构筑,所述的原则和做法可以实现在外部网-内部网-主机-用户体系上,形成多道防线,使企业计算机网络具有相当程度的安全性,从而达到企业信息系统的安全目标。     

网络媒体发展中的信息安全问题探讨

现代信息化的技术的进步给我们的社会生活带来的很大帮助,而网络媒体就是信息化技术的一个产物,它能迅速成为市场的一大宠儿,不仅有着他自身独有的优势和特点,还有与之相适应的外部条件,传统媒体在某些方面已经不能适应现代产业的发展,人们正在寻求一种迅速、容量大的传播方式,于是网络媒体的出现众望所归。但是由于我国的网络媒体的出现时间尚短,难免有着这样那样的问题,随着网络媒体为越来越多的人所接受,其问题也更加严重,尤其是传播不良的信息荼毒青少年的思想健康,网络受到黑客、病毒的侵袭,网上传播虚假信息、盗窃数据库资料等等,信息安全问题突出,这些问题越来越受到全世界的关注,成为全球共同注意的事情。     

2011年12月计算机病毒疫情分析

根据国家计算机病毒应急处理中心对互联网的监测,2011年12月计算机病毒整体感染情况呈平稳态势,主要传播途径仍以＂网络钓鱼＂和＂网页挂马＂为主。     

Human DDX3X Unwinds Japanese Encephalitis and Zika Viral 5′ Terminal Regions

Flavivirus genus includes many deadly viruses such as the Japanese encephalitis virus (JEV) and Zika virus (ZIKV). The 5′ terminal regions (TR) of flaviviruses interact with human proteins and such interactions are critical for viral replication. One of the human proteins identified to interact with the 5′ TR of JEV is the DEAD-box helicase, DDX3X. In this study, we in vitro transcribed the 5′ TR of JEV and demonstrated its direct interaction with recombinant DDX3X (K_d of 1.66 ± 0.21 µM) using microscale thermophoresis (MST). Due to the proposed structural similarities of 5′ and 3′ TRs of flaviviruses, we investigated if the ZIKV 5′ TR could also interact with human DDX3X. Our MST studies suggested that DDX3X recognizes ZIKV 5′ TR with a K_d of 7.05 ± 0.75 µM. Next, we performed helicase assays that suggested that the binding of DDX3X leads to the unwinding of JEV and ZIKV 5′ TRs. Overall, our data indicate, for the first time, that DDX3X can directly bind and unwind in vitro transcribed flaviviral TRs. In summary, our work indicates that DDX3X could be further explored as a therapeutic target to inhibit Flaviviral replication     

Lipid Droplet Motility Increases Following Viral Immune Stimulation

Lipid droplets (LDs) have traditionally been thought of as solely lipid storage compartments for cells; however, in the last decade, they have emerged as critical organelles in health and disease. LDs are highly dynamic within cells, and their movement is critical in organelle–organelle interactions. Their dynamics are known to change during cellular stress or nutrient deprivation; however, their movement during pathogen infections, especially at very early timepoints, is under-researched. This study aimed to track LD dynamics in vitro, in an astrocytic model of infection. Cells were either stimulated with a dsRNA viral mimic, poly I:C, or infected with the RNA virus, Zika virus. Individual LDs within infected cells were analysed to determine displacement and speed, and average LD characteristics for multiple individual cells calculated. Both LD displacement and mean speed were significantly enhanced in stimulated cells over a time course of infection with an increase seen as early as 2 h post-infection. With the emerging role for LDs during innate host responses, understanding their dynamics is critical to elucidate how these organelles influence the outcome of viral infection.     

甲型肝炎病毒(L-A-1株)基因型分析

目的 分析甲肝病毒基因型,为确定我国甲肝的分子流行病学及疫苗的安全性和免疫原性奠定基础。方法 采用抗原捕获聚合酶链反应（AC/PCR）,依据国际上甲肝病毒分型标准,扩增VP1/2A结合处的168个核苦酸（nt2909～3264）。结果 经生物信息学软件分析,得出L-A-1株病毒属于甲肝病毒基因IB亚型。结论 在我国有IA和IB两个亚型甲肝病毒流行。     

应用免疫策略对蒙脱石HIV DNA疫苗免疫作用的研究

目的运用Prime—Boost策略,研究蒙脱石(MMT)DAN疫苗对小鼠免疫功能的影响。方法选用3H-TdR掺入法测定MMT和DNA联合免疫小鼠脾淋巴细胞的增殖反应;采用ELISA试剂盒检测血清中的抗体和细胞因子。结果使用痘苗病毒和/或加入MMT加强免疫DNA疫苗后,均可以提高其体液和细胞免疫功能。结论“Prime-boost-boost”免疫方案在小动物模型中取得了较好的免疫效果。