Biofortification with selenium and lithium improves nutraceutical properties of major winery grapes in the Midwestern United States

Enriching the micronutrients, selenium (Se) and lithium (Li), in grapes to improve their nutraceutical properties were implemented by foliar application of organic fertiliser rich in Se and Li onto five grape cultivars. The effects of this biofortification on vine vigour, fruit quality, overall micronutrients and phenolic compounds also were investigated. Agronomic biofortification was found greatly increased the Se and Li content in the whole grape by multiple times, meanwhile it did not significantly affect the vine vigour and fruit quality of grapes. However, the biofortification did impact the Ionome (including all the mineral nutrients and trace elements) and phenolic compounds in grapes and this varied among cultivars. This study demonstrated foliar spray of organic Se/Li fertiliser was a very effective strategy to biofortify these micronutrients in grape berries, particularly in the skin, and therefore might be a promising strategy to increase the consumption and awareness of these grapes.     

Profile of Triacylglycerols,Phenols, and Vitamin E of Manjari Medika Grape Seed Oil and Cake: Introducing a Novel Indian Variety

The aim of the study is to investigate the biochemical composition of grapeseed oil and cake from an unexplored Indian grape‐juice cultivar, Manjari Medika (MM). The composition of oil and residual seed cake is evaluated using various chromatographic and spectroscopic techniques. The findings demonstrate that the vitamin E content of MM‐seed oil (1.15–1.35 g kg^?1) is distinctively higher than the Codex standard, suggesting its superior quality as an edible oil. The predominant triacylglycerols include trilinolein (LLL, 43%), dilinoleoyl‐stearylglycerol (LSL, 19%), and dilinoleoyl‐palmitoylglycerol (LLP, 11%), which are earlier recognized as natural antioxidants. The seed‐cake is rich in polyphenols including acylated anthocyanins (e.g., pelargonidin‐3‐O‐coumaroyl glucoside) and certain other flavonoids (e.g., catechin). The profile of phytonutrients in MM seed‐oil and cake is significantly superior to its seeded female parent and two other widely cultivated wine‐grape varieties. In brief, the studied by‐products of this new grape‐juice cultivar can be an important source of high‐value ingredients for use in food supplements, nutraceuticals, and functional foods. Practical applications: This study reports the phytochemical profile of the seed‐oil and seed cake derived from a newly developed grape variety, Manjari Medika. High contents of selective antioxidants: lipids, vitamin E, and phenols in the seed‐oil and cake with health benefits suggest their potential for use in nutraceutical and functional foods. These byproducts can be utilised as ingredients of functional foods and nutraceuticals (e.g., grape seed oil capsule) and also as raw materials in food supply chains (e.g., for production of grape cookies or cake). MM can also be utilized as a colorant in the food industry.     

Nonenzymic browning during storage of white hard grape pekmez (Zile pekmezi)

Effects of storage period on hydroxymethylfurfural, pH and colour were studied in white hard grape pekmez (Zile pekmezi), a Turkish traditional product. Pekmez samples were stored at 20 °C for 8 months. Analysis of variance revealed significant differences in hydroxymethylfurfural, pH, L, a and b values (P<0.01) based on storage time.     

Nephelometric study of salivary protein–tannin aggregates

Grape seed procyanidins were fractionated through different degrees of polymerisation, and human saliva was purified and separated into two fractions: one was mostly α‐amylase and the other was essentially proline‐rich proteins (PRPs). The interaction of these proteins with the procyanidin compounds was assayed using nephelometry, and the influence of several factors was investigated, such as degree of polymerisation, pH and concentrations of both protein and tannin. The same experiments were performed with bovine serum albumin (BSA). The amount of insoluble aggregates, resulting from the formation of polyphenol–protein aggregates, increased quickly up to a maximum value which thereafter remained practically unchanged. pH was set at 5.0 for all further assays, since it was the nearest value to that encountered in human saliva (pH 5.6–7.9), where proteins were stable and had a maximum ability to bind and precipitate procyanidin oligomers. These proteins were shown to have a strong affinity for procyanidin oligomers and were unable to resolubilise the polyphenol–protein aggregates when present in excess. PRPs required a much lower content to bind all the tannins (400 µg of procyanidin oligomers) than BSA and especially α‐amylase (48, 60 and 132 µg respectively). The procyanidin's ability to bind PRPs, BSA and α‐amylase increased with its average molecular weight. This ability increased regularly for PRPs up to 4500 Da, whereas the ability to bind the globular proteins decreased beyond 3400 Da. © 2001 Society of Chemical Industry     

葡萄籽油的提取方法及精炼工艺研究进展

葡萄酒生产过程中的下脚料,经过清洗、烘干等处理,分离出葡萄籽.所得葡萄籽通过压榨法、溶剂提取法或超临界流体萃取法得到葡萄籽毛油,所得毛油经脱胶、脱酸、水洗干燥、脱色、脱臭、特色过滤等精炼工艺最后得到精制成品油.本文对葡萄籽油的提取方法及精炼工艺研究进展进行了综述.     

层析法分离提取葡萄籽原花青素的进一步研究

以吸附量和解吸率为指标,比较了7种大孔吸附树脂对原花青素的静态吸附解吸性能并考查了AB—8树脂对原花青素的吸附特性及机理。实验结果表明:AB—8树脂是较理想的吸附剂;在溶液中AB—8树脂对原花青素的吸附达到平衡的时间为240 min,吸附行为符合Langmuir等温方程;最佳动态吸附参数原花青素提取液浓度4.0 mg/mL,吸附流速2 BV/h(1BV=13.03 mL)用40%乙醇溶液洗脱。AB—8树脂适合于柱层析操作分离原花青素。     

砾石土质条件下葡萄滴灌生育期土壤含水率变化规律研究

为探究酿酒葡萄在砾石土质条件下的土壤含水率变化规律及合理灌溉制度,选取贺兰山东麓砾石土典型试验区,以五年生赤霞珠品种为研究对象,设计2 550、2 850、3 225、3 600 m3/hm24个不同定额的灌水处理,应用TDR土壤水分剖面仪和土壤水势仪,监测生育期滴灌前后不同土层含水率与水势变化,针对监测数据从灌水处理整体与单个生育期不同角度进行分析,研究酿酒葡萄在砾石土条件下不同滴灌定额土壤含水率变化规律,最终提出生育期适宜灌溉制度。研究结果表明:随着灌溉定额的增加,土壤含水率在0~40cm土层范围内变化较明显;不同深度土层土壤水势变化规律与灌溉定额的大小有关;1m深土壤水分蓄存比并不是随着灌水量的增加而增大,而是当灌水量达到某一定额时,随着灌水量的增加,土壤水分蓄存比减小,砾石土单次灌水量高于300 m3/hm2时,土壤水分蓄存比较低,易产生深层渗漏。     

葡萄贮藏中SO2伤害与活性氧代谢的关系

以耐SO2品种巨峰和不耐SO2品种红地球葡萄为材料,研究了葡萄贮藏中SO2伤害与活性氧代谢的关系,结果表明,SO2伤害葡萄体内活性氧水平,GSH含理和ASP活性增加,SOD和CAT活性降低,与红地球相比,巨峰具有较高的SOD活性和GSH水平,受SO2伤害后,巨峰的ASP活性上升幅度大于红地球,其SOD活性和CAT活性的下降幅度低于红地球。     

咪鲜胺等新型杀菌剂对葡萄病害的防治效果

本文选用5种低毒杀菌剂对葡萄灰霉病和黑豆病进行了田间防治试验,结果表明,25%使百克1000倍液对葡萄灰霉病和黑豆病防治效果都最好,80%大生-M800倍液对灰霉病防效好,40%福星6000倍液和5%安福1000倍液对黑豆病防效好,32.5%丰收纯800倍液次之,其它供试药剂防效不理想。     

葡萄痕量灌溉技术试验研究

引进北京普泉公司痕量灌溉技术,建立葡萄井水痕量灌溉试验区,开展葡萄痕量灌溉试验研究。监测土壤湿润范围、土壤含水率变化、作物生长状况,并与大田葡萄沟灌进行试验对比。试验结果表明：痕量灌溉节水效果优越,作物根系层土壤长期处于适宜含水率范围,能够为作物生长创造良好的水环境,灌溉均匀度高于滴灌均匀度,研究提出了葡萄痕量灌溉制度。