高效液相色谱法同时测定保健食品中11种功效成分

建立高效液相色谱同时测定保健食品中11种功效成分的高通量分析方法。样品前处理采用甲醇-水-磷酸溶液提取,对油脂样品则采用甲醇-水-磷酸溶液与正己烷液液萃取除脂。采用Agilent TC-C18色谱柱(250 mm×4.6 mm,5μm),乙腈-2 g/L辛烷磺酸钠溶液(加入1 m L磷酸)作为流动相,流速1.0 m L/min,梯度洗脱,用二极管阵列检测器串联荧光检测器检测,外标法峰面积定量。VB2在0.25~25 mg/L、VB6在0.5~20 mg/L、烟酸、烟酰胺在1~100 mg/L、其余5种功效成分在0.5~50 mg/L质量浓度范围内线性良好,相关系数r为0.999 1~1.000 0。在样品中待测成分含量约0.5、1倍和2倍3个水平的添加回收率为86.4%~110%之间,相对标准偏差为0.21%~11.7%。方法的定量限:烟酸、烟酰胺为20 mg/kg,其余9种功效成分方法的定量限为10 mg/kg。     

Systems pharmacology approach to investigate the molecular mechanisms of herb Rhodiola rosea L. radix

Rhodiola rosea L. radix (RRL) is one of the most popular medical herb which has been widely used for the treatment of different diseases effectively, including cardiovascular diseases and nerve system diseases. However, due to the multiple compounds in RRL, the underlying molecular mechanisms of RRL are remained unclear. To decipher the action mechanisms of RRL from a systematic perspective, a systems pharmacology approach integrated absorption, distribution, metabolism, and excretion (ADME) system, drug targeting, and network analysis was introduced. First, by the ADME screening system and the target fishing process, 56 potential active compounds and 62 targets were obtained, respectively. In addition, compound-target network demonstrated that most compounds interacted with multiple targets, indicating that RRL may enhance its therapeutic effects probably through hitting on multiple targets in a holistic level. Moreover, target-pathway network and gene ontology analysis showed that multiple targets of RRL were involved in several biological pathways, i.e. Neuroactive ligand-receptor interaction, calcium signaling pathway, adrenergic signaling in cardiomyocytes, and VEGF signaling pathway, which dissecting the therapeutic effects of RRL on various diseases, such as cardiovascular diseases, depression, adaptation diseases, etc. In summary, this work successfully explains the potential active compounds and the multi-scale curative action mechanisms of RRL for treating various diseases; meanwhile, it implies that RRL could be applied as a novel therapeutic agent in arthritic diseases. Most importantly, this work provides an in silico strategy to understand the action mechanisms of herbal medicines from molecular/system levels, which will promote the new drug development of traditional Chinese medicine.     

食用乙醇常温浸提法提取红景天苷

以超临界CO_2萃取后的高山红景天干粉为原料,进行食用乙醇常温浸提法提取红景天苷的工艺研究,采用正交实验设计筛选出最佳的提取工艺参数为：乙醇浓度为40%,溶剂和原料之比为6：1。常温浸提时间为48h,颗粒度为40目。     

利用LC-MS对保健酒中七种成分的定性分析研究

分别对神酒、健身酒、海马酒这3种保健酒中是否含有红景天苷、鲁斯可皂苷元、人参皂苷Rb1、腺苷、紫丁香苷、羟基红花黄色素和山奈素这7种成分进行定性研究。用LCMS-8050分别对3种保健酒和7种成分标准品进行分析,并对所得结果进行了比较总结。结果表明,3种保健酒中均检测出红景天苷、人参皂苷Rb1、腺苷和紫丁香苷;3种保健酒中均未检测出鲁斯可皂苷元和山奈素;健身酒和神酒中检测出羟基红花黄色素。     

红景天活性成分及药理作用

红景天为景天属植物,种类较多,分布广。其主要化学成份有红景天苷、酪醇及红景天多糖,并且含有黄酮类化合物、酚类化合物、蛋白质、脂肪、微量挥发油及微量元素、氨基酸等。研究表明,红景天具有抗缺氧、抗疲劳、抗衰老、抗辐射、调节神经系统、心脑血管系统和免疫力等功效。现就其化学成分及药理作用作一综述。     

红景天苷的溶剂浮选

建立了一种测定红景天苷的新方法,即采用溶剂浮选法分离富集红景天苷,用紫外分光光度法测定其含量.考察了浮选溶剂、试液pH、氮气流速、浮选时间及电解质NaC1等因素对浮选效果的影响,优选出最佳浮选条件.采用所述方法对不同前处理样品中红景天苷含量进行测定,样品加标回收率为99.71％～104.21％,RSD为1.6～2.21...     

高效液相色谱法测定长鞭红景天中红景天苷和酪醇含量

本文建立了高效液相色谱法快速测定长鞭红景天中红景天苷和酪醇含量的方法。色谱柱AgilentRP-C18(3.9×50mm,3.5μm);流动相为水-甲醇(80:20);流速为1ml/min;检测波长276nm。红景天苷和酪醇在1.5～30μg和0.8～16μg范围内线性关系良好(r=0.9999),平均回收率分别为98.5%和98.2%。实验3min即可完成测定,方法简便,快速,结果可靠,可用于优良单株的快速筛选。     

长白山地区高山红景天中红景天苷含量及农药残留的测定

为了解长白山地区高山红景天药材的质量,对产自长白山地区不同年限的人工栽培和野生高山红景天中的红景天苷含量和农药残留量进行了测定.在检测波长为275nm、色谱柱为PLATISTLTM C18柱(250mm×4.6mm,5μm)、流动相为甲醇-乙酸铵溶液、线性度(r)高于0.999 8、平均回收率为96.62%、相对标准偏差为1.32%的条件下,采用高效液相色谱法测定了红景天苷的含量;在色谱柱为HP-5毛细管气相色谱柱(0.32mm×30m×0.25μm)、进样口温度为250℃、Ni63电子捕获检测器温度为300℃、尾吹15mL/min、柱温以10℃/min升至220℃并保持20min条件下,采用气相色谱法测定了农药残留.结果表明,不同年限人工栽培品的红景天苷含量有差异,且含量均低于野生品,样品中均无六六六、滴滴涕农药残留.     

Development of Lipid-Shell and Polymer Core Nanoparticles with Water-Soluble Salidroside for Anti-Cancer Therapy

Salidroside (Sal) is a potent antitumor drug with high water-solubility. The clinic application of Sal in cancer therapy has been significantly restricted by poor oral absorption and low tumor cell uptake. To solve this problem, lipid-shell and polymer-core nanoparticles (Sal-LPNPs) loaded with Sal were developed by a double emulsification method. The processing parameters including the polymer types, organic phase, PVA types and amount were systemically investigated. The obtained optimal Sal-LPNPs, composed of PLGA-PEG-PLGA triblock copolymers and lipids, had high entrapment efficiency (65%), submicron size (150 nm) and negatively charged surface (−23 mV). DSC analysis demonstrated the successful encapsulation of Sal into LPNPs. The core-shell structure of Sal-LPNPs was verified by TEM. Sal released slowly from the LPNPs without apparent burst release. MTT assay revealed that 4T1 and PANC-1 cancer cell lines were sensitive to Sal treatment. Sal-LPNPs had significantly higher antitumor activities than free Sal in 4T1 and PANC-1 cells. The data indicate that LPNPs are a promising Sal vehicle for anti-cancer therapy and worthy of further investigation.     

红景天保健面包的研制(Ⅰ)

红景天是一种食药兼用植物，具有多种保健功能。利用其干燥根茎经乙醇提取后可得到含功能因子红景天苷1.1%的提取物，作为功效成分应用于面包生产中，结果表明：面粉中添加1％－3％的红景天提取物，对面包的感官质量无不良影响，可增加面包的持水性，减缓老化。其口感良好、风味独特，并具有保健作用。