兰州市文化产业发展现状的SWOT分析

文化产业是21世纪具有战略地位的朝阳产业。兰州市拥有丰富的历史文化资源,文化产业将成为兰州未来经济发展的主导产业。运用SWOT分析法对兰州市文化产业发展现状进行综合分析,并提出兰州市文化产业发展的策略和建议。     

响应面法优化超声波辅助提取百合花秋水仙碱工艺

以百合花为研究对象,利用响应面分析法优化百合花中秋水仙碱的超声辅助提取工艺。通过单因素实验分别考察乙醇体积分数、提取时间、提取温度和料液比对百合花秋水仙碱含量的影响。选取适当的实验因素水平,利用Design Expert软件和Box-Behnken设计法设计响应面实验,对各个因素的显著性和交互作用进行分析。结果表明超声提取百合花秋水仙碱的最佳工艺条件为:60%乙醇为提取溶剂、温度50℃、料液比为1∶21、提取时间为33min,该条件下提取的秋水仙碱含量为16.25μg/g。      

响应面法优化兰州百合多糖的提取工艺

采用水提醇沉法对兰州百合多糖进行提取,选取提取温度、提取时间、料液比三个因素作为研究对象,以兰州百合多糖的得率为评价指标,在单因素实验的基础上,通过Box-Behnken响应面分析法优化兰州百合多糖的提取工艺条件。最终确定最佳的提取条件为:提取温度为64.7℃、提取时间为6.12h、料液比为1∶30,在此提取条件下,兰州百合多糖的理论提取率可达到12.37%。      

贮藏温度和包装材料对百合保鲜包装品质的影响

采用不同贮藏温度(30℃和5℃)和包装材料(PE、OPP、OPP/PE膜)对百合进行保鲜包装,测定不同贮藏时间下的百合球茎Vc含量、总糖含量、色差、失重率和感官品质变化。结果表明:低温能够显著抑制百合失重,保持其较好的色泽和总糖含量。不同包装材料比较,具有较高透气率的PE、OPP膜保鲜效果较好,OPP/PE膜次之。低温与包装之间有较好的协同作用。     

睡莲花提取物对酪氨酸酶的抑制作用及其动力学研究

采用紫外分光光度计测定多巴醌,得到了睡莲花提取物对酪氨酸酶的抑制率;以L-酪氨酸为底物,采用酶动力学的方法研究了睡莲花提取物对酪氨酸酶单酚酶活性的抑制作用及抑制机制。结果表明,睡莲花提取物对酪氨酸酶具有明显抑制作用,其中30%乙醇洗脱部位抑制效果最好,其对酪氨酸酶单酚酶活性的抑制作用表现为可逆反竞争型抑制;50%乙醇洗脱部位对酪氨酸酶单酚酶活性的抑制作用表现为可逆性抑制,低浓度时为混合型,高浓度时为竞争型。     

基于步行者视角的兰州市步行环境研究

近年来,伴随着各地区的城市规划建设热潮,步行空间被挤占、步行环境恶化问题不断涌现。本文基于对兰州市四个区域520位出行者的问卷调查,分析城市步行环境存在问题及规划的必要性并提出部分建议,以期对城市规划有一定的借鉴意义。研究表明:①现代交通体系中,步行仍是居民的首要出行方式,出行时间与年龄影响出行方式的选择。②收入水平及出行目的决定步行者需求,影响着步行者对步行环境的满意度。③步行环境问题表现出明显的区域差异。④城市步行环境规划必须明确观念、原则、对象,抓落实,勤管理。     

3种百合淀粉主要理化性质的研究

对湖南株洲和宜章两地的卷丹及麝香百合的淀粉的主要理化性质进行了研究。其结果表明:株洲、宜章卷丹及麝香百合淀粉的淀粉含量分别为78·74%、87·33%、78·36%,其直链淀粉分别为24·36%、26·04%、21·38%;淀粉晶体结构是B型结构;糊化温度分别为51·9~54·3℃、55·7~61·0℃、57·3~60·7℃。随着加热温度的上升,百合淀粉的膨胀度和溶解度均呈增加趋势,在65℃时膨胀较小,在75~95℃时膨胀较快,为典型的二段膨胀过程,属限制型膨胀淀粉。     

Expression Patterns of Sugar Transporter Genes in the Allocation of Assimilates and Abiotic Stress in Lily

During the growth cycle of lilies, assimilates undergo a process of accumulation, consumption and reaccumulation in bulbs and are transported and allocated between aboveground and underground organs and tissues. The sink–source relationship changes with the allocation of assimilates, affecting the vegetative growth and morphological establishment of lilies. In this study, the carbohydrate contents in different tissues of five critical stages during lily development were measured to observe the assimilates allocation. The results showed bulbs acted as the main source to provide energy before the budding stage (S3); after the flowering stage (S4), bulbs began to accumulate assimilates as a sink organ again. During the period when the plant height was 30cm with leaf-spread (S2), leaves mainly accumulated assimilates from bulbs through the symplastic pathway, while when leaves were fully expanded, it transformed to export carbohydrates. At the S4 stage, flowers became a new active sink with assimilates influx. To further understand the allocation of assimilates, 16 genes related to sugar transport and metabolism (ST genes) were identified and categorized into different subfamilies based on the phylogenetic analysis, and their protein physicochemical properties were also predicted. Tissue-specific analysis showed that most of the genes were highly expressed in stems and petals, and it was mainly the MST (monosaccharide transporter) genes that were obviously expressed in petals during the S4 stage, suggesting that they may be associated with the accumulation of carbohydrates in flowers and thus affect flower development process. LoSWEET14 (the Sugar will eventually be exported transporters) was significantly correlated with starch in scales and with soluble sugar in leaves. Sugar transporters LoHXT6 and LoSUT1 were significantly correlated with soluble sugar and sucrose in leaves, suggesting that these genes may play key roles in the accumulation and transportation of assimilates in lilies. In addition, we analyzed the expression patterns of ST genes under different abiotic stresses, and the results showed that all genes were significantly upregulated. This study lays a solid foundation for further research on molecular mechanism of sink–source change and response to abiotic stresses in lilies.     

食用百合高效生态栽培技术

文章从食用百合的经济意义入手,分析了现阶段百合生产存在的问题,总结了食用百合高效生态栽培技术,期望为食用百合的栽培提供理论基础与实践经验。