Photoregulation of Gene Expression with Ligand-Modified Caged siRNAs through Host/Guest Interaction

Small interfering RNA (siRNA) can effectively silence target genes through Argonate 2 (Ago2)-induced RNA interference (RNAi). It is very important to control siRNA activity in both spatial and temporal modes. Among different masking strategies, photocaging can be used to regulate gene expression through light irradiation with spatiotemporal and dose-dependent resolution. Many different caging strategies and caging groups have been reported for light-activated siRNA gene silencing. Herein, we describe a novel caging strategy that increases the blocking effect of RISC complex formation/process through host/guest (including ligand/receptor) interactions, thereby enhancing the inhibition of caged siRNA activity until light activation. This strategy can be used as a general approach to design caged siRNAs for the photomodulation of gene silencing of exogenous and endogenous genes.     

Identification of Host Cellular Protein Substrates of SARS-COV-2 Main Protease

The novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of coronavirus disease-19 (COVID-19) being associated with severe pneumonia. Like with other viruses, the interaction of SARS-CoV-2 with host cell proteins is necessary for successful replication, and cleavage of cellular targets by the viral protease also may contribute to the pathogenesis, but knowledge about the human proteins that are processed by the main protease (3CLpro) of SARS-CoV-2 is still limited. We tested the prediction potentials of two different in silico methods for the identification of SARS-CoV-2 3CLpro cleavage sites in human proteins. Short stretches of homologous host-pathogen protein sequences (SSHHPS) that are present in SARS-CoV-2 polyprotein and human proteins were identified using BLAST analysis, and the NetCorona 1.0 webserver was used to successfully predict cleavage sites, although this method was primarily developed for SARS-CoV. Human C-terminal-binding protein 1 (CTBP1) was found to be cleaved in vitro by SARS-CoV-2 3CLpro, the existence of the cleavage site was proved experimentally by using a His₆-MBP-mEYFP recombinant substrate containing the predicted target sequence. Our results highlight both potentials and limitations of the tested algorithms. The identification of candidate host substrates of 3CLpro may help better develop an understanding of the molecular mechanisms behind the replication and pathogenesis of SARS-CoV-2.     

CN decoration of dibenzofuran modified biphenyl for high triplet energy host for blue phosphorescent organic light-emitting diodes

A strongly electron deficient and high triplet energy host for blue emitters was developed by decorating a dibenzofuran modified biphenyl backbone structure with multiple CN units. Two hosts, 6,6′-bis(6-cyanodibenzo[b,d]furan-4-yl)-[1,1′-biphenyl]-3,3′-dicarbonitrile(CNDBF1) and 2,2′-bis(6-cyanodibenzo[b,d]furan-4-yl)-[1,1′-biphenyl]-4,4′-dicarbonitrile(CNDBF2), were derived from the CN decoration strategy for application in blue organic light-emitting diodes requiring high triplet energy host. They showed high triplet energy above 2.79 eV and acted as the electron transport type host based on the strong electron deficiency. The mixture of the CNDBF1 and CNDBF2 hosts with a hole transport type 3,3′-di(9H-carbazol-9-yl)-1,1′-biphenyl host performed as the exciplex host of a blue phosphor and accomplished high external quantum efficiency of 22.7% in the blue phosphorescent organic light-emitting diodes.     

Adsp-21060的主机接口在实时图像处理中的应用

简要介绍了数字信号处理器Adsp 2 10 6 0和大规模可编程逻辑器件EP1K5 0。详细讨论了Adsp 2 10 6 0的主机接口工作模式以及一个实时图像跟踪处理器的硬件组成原理 ,在这个系统中EP1K5 0充当了主机。调试结果表明 ,所提出的硬件结构设计思想工作效率高 ,完全能够胜任实时图像处理的实际需要。     

几种涉密网主机系统保护及审计技术的比较

论文比较详细地阐述了涉密网主机系统所面临的安全隐患,提出了涉密网主机安全防护所要解决的几个技术问题,并就可能实现的技术方法进行了探讨和比较。     

Biotransformation of l‐menthol by twelve isolates of soil‐borne plant pathogenic fungi (Rhizoctonia solani) and classification of fungi

The microbial transformation of l‐menthol ( 1 ) was investigated by using 12 isolates of soil‐borne plant pathogenic fungi, Rhizoctonia solani (AG‐1‐IA Rs24, Joichi‐2, RRG97‐1; AG‐1‐IB TR22, R147, 110.4; AG‐1‐IC F‐1, F‐4, P‐1; AG‐1‐ID RCP‐1, RCP‐3, and RCP‐7) as a biocatalyst. Rhizoctonia solani F‐1, F‐4 and P‐1 showed 89.7–99.9% yields of converted product from 1 , RCP‐1, RCP‐3, and RCP‐7 26.0–26.9% and the other isolates 0.1–12.0%. In the cases of F‐1, F‐4 and P‐1, substrate 1 was converted to (?)‐(1S,3R,4S,6S)‐6‐hydroxymenthol ( 2 ), (?)‐(1S,3R,4S)‐1‐hydroxymenthol ( 3 ) and (+)‐(1S,3R,4R,6S)‐6,8‐dihydroxymenthol ( 4 ), which was a new compound. Substrate 1 was converted to 2 and/or 3 by RRG97‐1, 110.4, RCP‐1, RCP‐3 and RCP‐7. The structures of the metabolic products were elucidated on the basis of their spectral data. In addition, metabolic pathways of the biotransformation of 1 by Rhizoctonia solani are discussed. Finally, from the main component analysis and the differences in the yields of converted product from 1 , the 12 isolates of Rhizoctonia solani were divided into three groups based on an analysis of the metabolites. Copyright © 2003 Society of Chemical Industry     

基于LotusNotes的文档ID应用剖析

介绍了文档数据库Lotus Domino／Notes的特点．对其文档处理的核心，即Notes文档的ID构成机制作了系统剖析．并在此基础上分析了如何在文档复制中运用ID的不同组件以及如何运用API程序访问文档ID等。     

光电设备电子机柜布线工艺研究

作为信号和电能载体的导线，同时也是噪声的载体和外来噪声侵入设备的媒体。描述了噪声通过导线侵入设备内部的方式，通过降低噪声的途径确定了机柜布线的原则，介绍了布线的要求和方法，最后提出了用布线槽布线的建议。     

用PB和ORACLE方法实现身份证号升位

在对人员的管理工作中 ,需经常使用姓名和身份证号来进行人员检索。身份证号所具有的唯一性 ,决定了它成为表征一个人信息的准确特征。原有的 15位身份证号存在着“2 0 0 0年”问题 ,需升至 18位。本文分别用PB和ORACLE两种语言方式 ,讲述了实现身份证号升位的方法。     

身份管理渐行其道

目前人们面临的网络安全问题越来越严峻，不仅包括计算机病毒、垃圾电子邮件等的挑战，对信息资源的有效存取保护要求也在提高。身份管理技术能对用户身份标识进行集中与简化管理，实现安全、简便登录等功能，不但削减了投入和维护成本，还提高了效率，因此近来得到迅速发展。2003年的调查显示，身份管理在安全产品市场增幅达45％；在各企业应用普及率已达到17.9％。但身份管理还需要解决提高单点登录安全性，实施多方可靠数字身份以及本身复杂性等问题。