GOLPH3 Regulates EGFR in T98G Glioblastoma Cells by Modulating Its Glycosylation and Ubiquitylation

Protein trafficking is altered when normal cells acquire a tumor phenotype. A key subcellular compartment in regulating protein trafficking is the Golgi apparatus, but its role in carcinogenesis is still not well defined. Golgi phosphoprotein 3 (GOLPH3), a peripheral membrane protein mostly localized at the trans-Golgi network, is overexpressed in several tumor types including glioblastoma multiforme (GBM), the most lethal primary brain tumor. Moreover, GOLPH3 is currently considered an oncoprotein, however its precise function in GBM is not fully understood. Here, we analyzed in T98G cells of GBM, which express high levels of epidermal growth factor receptor (EGFR), the effect of stable RNAi-mediated knockdown of GOLPH3. We found that silencing GOLPH3 caused a significant reduction in the proliferation of T98G cells and an unexpected increase in total EGFR levels, even at the cell surface, which was however less prone to ligand-induced autophosphorylation. Furthermore, silencing GOLPH3 decreased EGFR sialylation and fucosylation, which correlated with delayed ligand-induced EGFR downregulation and its accumulation at endo-lysosomal compartments. Finally, we found that EGF failed at promoting EGFR ubiquitylation when the levels of GOLPH3 were reduced. Altogether, our results show that GOLPH3 in T98G cells regulates the endocytic trafficking and activation of EGFR likely by affecting its extent of glycosylation and ubiquitylation.     

基于多线程技术的监控系统的设计

详细介绍在32位操作系统Windows95／98下，用C Builder3．0开发基于多线程技术的监控系统的有关方法。     

EPSW98在地形测量中的应用

EPSW98是面向对理信息系统（简称GIS）的GIS前端软件，可实现内外业一体化，能与GIS数据、CAD数据相互转换，达到一图多用的目的。对EPSW98系统概述、作业流程、应用技巧作了详细论述。     

精心维护电脑三步曲

本文针对安装ＷＩＮ２０００ Ｐｒｏｆｅｓｓｉｏｎａｌ的个人计算机安全维护，从三个方面提出几点建议。     

无线智能网技术

本介绍CDMA无线智能网（WIN）的产生背景、技术标准、提供的业务、与GSM移动智能网技术上的差异以及它在实际工程中的应用，最后对WIN的发展进行展望。     

基于PDA的血细胞自动计数系统的研究

为克服现有血细胞计数仪结构复杂、体积大的缺点,介绍了一种基于个人数字助理(PDA)的便携式血细胞自动计数系统.本系统具有操作简便、体积小、准确度高等优点,能够满足野外血细胞计数检验的需要.系统包括显微镜、CCD摄像头、PDA,并采用eVC++4.0编程,实现了WIN CE操作系统下细胞图片的实时观察与处理,直接获得细胞...     

Necessary optimality conditions for fuzzy variational problems

The main aims of this study are to derive the fuzzy Euler-Lagrange conditions for both fuzzy unconstrained and constrained variational problems based on the concepts of differentiability and integrability of a fuzzy mapping that may be parameterized by the left and right-hand functions of its α-level sets.     

Windows98环境下实时控制系统的开发

针对Windows98环境下开发实时控制系统等有关内容进行了分析,指出仅仅通过Windows中提供的定时器要完成高精度的实时控制是不可能的,必须从软、硬件两方面加以解决,提出了两种编写中断处理程序的方法;开发虚拟设备驱动程序和使用TVicHW32控件。     

机房软件系统维护探讨

主要探讨通过系统恢复和系统重装两种途径来解决Windows98系统运行不稳定甚至崩溃的问题，介绍了使用“系统件检查器”和通过修复注册表来恢复系统，以及采用释放分区克隆件、通过硬盘安装以及网上邻居安装Windows98系统来重装系统。     

PS型数据采集接口板卡的驱动模块开发

采用Vineo Sofware公司出品的VxD开发软件包VtoolsD，开发出了Windows98环境下的数据采集卡虚拟设备驱动程序，VxD部分完成对物理设备的直接控制和通讯，DLL部分完成设备驱动（Ring0)与应用程序（Ring3)之间的接口，Microsoft Visual C 开发的应用程序通过调用Windows系统函数，实现对采样的精确定时，其周期最小可达0．1ms。