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用褐藻酸钙对小新月菱形藻进行固定化培养实验,测定不同胶粒大小、胶珠密度、CaCl2浓度及不同接种量对该藻的影响,比较了自由化生长与固定化细胞的生长曲线。小新月菱形藻在褐藻酸钙凝胶中仍具有呼吸和光合作用的能力。球径为3.5mm,CaCl2浓度为2%时,细胞生长快,每50mL培养液中加入200个胶珠时细胞生长量大,接种量不能低于104个细胞/mL。与游离的小新月菱形藻相比,固定化小新月菱形藻生长慢,但生长周期长。 相似文献
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以NaNO3为氮源,研究了氮浓度的五个水平及光照强度对小新月菱形藻的生长率及总脂含量的影响.结果表明:小新月菱形藻(Nitzschia closterium f.minutissima)(MACC/B222)在氮浓度为32mmol/L时平均生长率∥达到最大值0.7100d,脂肪含量在16mmol/L时达到最大值34.8%;在光强260-μmol/(s·m^2)处获得最大生长率0.6505d^-1,140μmol/(s·m^2)处获得最大总脂含量33.9% 相似文献
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Karchiyappan Thirugnanasambandham 《Energy Sources, Part A: Recovery, Utilization, and Environmental Effects》2018,40(12):1461-1468
In the present work, a new and pioneering hybrid technology, called hydrodynamic-cavitation reactor, was established and investigated to proof the feasibility for the biodiesel production from Chlorella minutissima microalgae. The process parameters such as inlet pressure (A), molar ratio (B), catalyst concentration (C), and reaction time (D) have been investigated over the biodiesel yield from Chlorella minutissima microalgae. Box–?Behnken design was applied to develop the second- order polynomial model. The error between experimental values and model prediction was found to be less than 10%. Interactive effects of process variables on the biodiesel yield from Chlorella minutissima microalgae was studied using contour graphs. Inlet pressure of 4 bar, molar ratio of 1: 30, catalyst concentration of 1.3%, and reaction time of 40 min produced 99% of biodiesel yield. Further, a kinetic model has also been developed and considers the transesterification reaction to be a second-order reversible, first order with respect to each of the reactants and products. Estimated values of kinetic constants are k1 = 0.00014 L min/mol and k2 = 0.035 L min/mol. 相似文献
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