丝素和丝胶的凝胶特性及其结构

桑蚕丝素和丝胶溶液在一定的条件下会形成凝胶。其凝胶化的过程与温度、浓度、溶液的PH值有关。温度和浓度的提高有利于凝胶化速度的加快，丝素在PH3～4时最容易形成凝胶．而丝胶则在PH6～7时最易形成凝胶；凝胶的强度也和上述因素有关。文章并指出凝胶化实质上是丝素和丝胶大分子p化的过程。     

真丝绸新型快速精练剂与应用工艺

介绍了ZS系列丝素保护快速精练剂的应用范围和性能，并与国内外常用精练剂作了对比试验。结果表明；它能保护真丝绸在碱性条件下的强力不受损伤；若采用合适的工艺条件．能缩短精练时间，提高产品质量，降低成本，提高生产效率。     

功能性丝素共混膜的研究现状与进展

丝素是一种性能优良的天然高分子，在生物、医药等很多方面有着广泛的应用。本文综述了国内外学者在丝素的结构、性能尤其在共混方面的研究及成果。     

棉织物的丝素防皱整理

将丝素整理剂与水溶性聚氨酯复配，对棉织物进行防皱整理。结果表明，两者之间有较好的协同增效作用，整理品的抗皱性明显改善，且白度、强力保留率以及手感等均达到满意效果。     

胶原/丝素膜的制备及其SO2等离子体改性研究

利用丝素和胶原蛋白各自的优点,制备了胶原一丝素共混膜,并通过FTIR、TGA、SEM等手段对其结构进行了表征。选用SO2采用低温等离子体技术对胶原／丝素膜进行了表面处理。材料的体外抗凝血性能由体外凝血时间——凝血酶原时间（PT）、部分凝血活酶时间（APTT）、凝血酶时间（TT）作为评价标准。结果表明,共混膜中二组分间存在的分子间作用力,加入小于50％的丝素的胶原膜经乙醇处理后与纯胶原膜相比,其力学性能及热稳定性有所改善。通过改变丝素比例可以调整共混膜的吸水性。经SO2处理后由于在共混膜表面引入了磺酸基团,因而具有良好的抗凝血性能。     

Three-dimensional fibroin/collagen scaffolds derived from aqueous solution and the use for HepG2 culture

Although three-dimensional fibroin scaffolds have been prepared with freeze–drying method, these scaffolds still cannot meet the requirements of tissue engineering. In this article, a new process is described to form fibroin-based porous scaffolds with controllable structure and morphological features. When collagen was added to fibroin solution, the viscosity of the blend solution increased because of the interaction between fibroin and collagen, and then it restrained the unwanted fibroin leaf formation in freezing process that generally appeared in the previous fibroin scaffold preparation. With methanol treatment, the fibroin/collagen scaffolds became water-stable, following the transition from random and -helix to β-sheet conformation. The aqueous-fibroin porous scaffolds had highly homogeneous and interconnected pores with pore sizes ranging from 127 to 833 μm, depending on the fibroin concentration. The porosity of scaffolds was >90%, and the yield strength and modulus were up to 354±25 kPa and 30±0.1 MPa, respectively, when the blend solution, containing 20% collagen, maintained 4% fibroin concentration. Adhesion, spreading and proliferation of HepG2 cells on fibroin and fibroin/collagen blend scaffolds were also observed to investigate the biocompatibility. Scanning electron microscopy (SEM) and MTT analyses demonstrated that the adding of collagen evidently facilitated HepG2 attachment and proliferation in vitro. These new fibroin based three-dimensional scaffolds provided much more excellent properties due to the greatly improved control of pore size, the uniform pore distribution, the hydrophility, the mechanical properties and the biocompatibility compared with those of reported three-dimensional fibroin scaffolds.     

Acceleration effect of sericin on shear-induced β-transition of silk fibroin

Although silk sericin (SS) occupies 25% of silk protein, its importance has often been overlooked in the natural silk spinning process and in the formation of the crystalline structure of silk fibroin (SF). In this study, we elucidated the role of SS in the crystallization process of SF under shear using SF/SS blend solutions. In order to apply shear stress to the blend solution, a rotating glass rod was inserted into a glass tube filled with the solution and the shear rate was determined to be in the range of 598–724 s⁻¹. After shearing, SF aggregates were formed and the amount of the aggregates increased with shearing time. Additionally, it was observed that the aggregate formation and β-sheet transition of SF were enhanced when a proper amount of SS was in the blend solution. Consequently, the SS considerably contributes to the structural transition of SF under shear. The SS can improve the shear-induced β-sheet transition and crystallization of SF.     

丝素蛋白膜膜电位和离子透过量的理论模拟

蚕丝丝素膜是一种含有碱性氨基酸和酸性氨基酸的两性弱荷电膜。用有关强荷电膜的ＴＭＳ理论可以计算荷电膜的膜电位、离子的透过系数和膜的一些重要参数。经过一些假定后，用理论计算结果来模拟实验值，得到了丝素膜的有效荷电密度约为０．０１５ｍｏｌ／Ｌ，以及某些离子在丝素膜中的移动度等重要参数。由此得知，用丝素膜可以控制一定浓度的可离子化药物和离子的渗透速度。     

碳酸钙-丝素粉体材料结构性能的探究

制备了不同质量分数的碳酸钙-丝素粉体材料,利用差热分析(DTA)、傅里叶红外光谱(FTIR)、X射线衍射(XRD)、扫描电镜(SEM)测试技术,探究了不同质量分数对粉体中丝素蛋白凝聚态结构和碳酸钙晶型的影响。结果表明:碳酸钙对丝素蛋白凝聚态结构的影响显著,当碳酸钙质量分数过大时,丝素蛋白的β-折叠成分会减少;同时研究发现,控制丝素蛋白的质量分数可以获得粒径较小、分散性能良好的立方形或球形碳酸钙。     

Growth of fibroblast and vascular smooth muscle cells in fibroin/collagen scaffold

In our recent study, a novel fibroin/collagen scaffold with improved mechanical properties and controllable porous structure was prepared through freeze–drying method. In this research, the cyto-compatibility was further studied, using fibroblast and vascular smooth muscle cells (VSMC) as the model cells. MTT results indicated that the growth of fibroblast and VSMC both further improved in the fibroin/collagen scaffold than in pure fibroin scaffolds. The confocal and SEM results showed that fibroblast cells and VSMCs had better adhesion and spreading properties in the fibroin/collagen scaffolds. Although further studies, such as the extracellular matrix production and the functional gene expression, are necessary to clarify the biocompatibility of the fibroin/collagen scaffolds, the present results indicate that the fibroin/collagen scaffold is a new scaffold material suitable for tissue engineering. On the other hand, the mild and all-aqueous preparation processes also make it possible to embed different growth factors inside the scaffolds to maximize cell functions and the formation of specific tissues.