蜂胶中具有抗氧化活性的木脂素

利用柱层析方法从蜂胶中分离得到了4种呋喃骈呋喃类木脂素，利用波谱学方法鉴定为芝麻脂素（sesamin）、yangambin、（＋）－松脂素[( )-pinoresinol]和（＋）－丁香脂素[( )-syringaresinol]，其中（＋）－松脂素和（＋）－丁香脂素为首次从蜂胶中得到。利用测定小鼠肝细胞微粒体脂质过氧化抑制作用的方法，检测了所得到的4种木脂素的抗氧化活性，其IC50值范围为9.0-14.0μg/ml。     

天然“药品”——蜂胶

主要介绍了蜂胶的营养成分、分类、质量标准、提取方式及保健功能。     

Encapsulation of propolis flavonoids in a water soluble polymer using pressurized carbon dioxide anti-solvent crystallization

This study investigated the pressurized carbon dioxide anti-solvent co-precipitation process (abbr. PAS) on encapsulation of propolis with water soluble polyethylene glycol (PEG). The extent to which recovery of propolis flavonoids, total yield, amount of propolis (i.e. drug content) affects the precipitation was examined using a two-factor central composite schemed experimental design method. Analysis results indicated that the drug content and total yield of the precipitates are conversely related to each other in the PAS process. Additionally, micro-sized amorphous particulates of propolis encapsulated on the surface of PEG were generated, as evidenced by chromatography and X-ray diffraction analyses. Total yield of the PAS co-precipitation process reached 88%, and recovery of propolis flavonoids achieved 97%. Experimental results also indicated that the concentration ratio of propolis to PEG in the feed more significantly affects the drug content than that of the pressure factor. Moreover, nearly spherical and aggregated micro-sized co-precipitated particulates are more soluble in an aqueous solution than those of ethanol extracts.     

蜂胶在食用油脂中的抗氧化作用

本文以猪油、菜油为实验材料,测定其POV值来评价蜂胶在动物油和植物油中的抗氧化特性,并与没食子酸丙酯(PG)比较.结果表明蜂胶能够延缓这些底物的氧化进程,明显延长诱导期.且柠檬酸、卵磷脂能增强其抗氧化功能.蜂胶的抗氧化能力强于相近浓度的PG,蜂胶在短时间高温加热后,其抗氧化效率不受影响.     

蜂胶总黄酮的提取最佳工艺研究

对蜂胶毛胶的总黄酮提取工艺进行了研究,在单因素实验和正交试验的基础上,得出最佳的提取条件为:30℃下,100%乙醇为提取剂,料液比为1:14,,提取时间48h,一次提取率高达92.25%。     

Development and validation of HPLC method for determination of salicin in poplar buds: Application for screening of counterfeit propolis

The main plant origins of propolis are the populus species and their hybrids, both located in China. Poplar tree gum, the extract of populus buds, has been widely used as counterfeit propolis, but no efficient method was known for detecting the counterfeit. Salicin is a characteristic marker of the genus populus, which may be hydrolysed by β-glucosidase during propolis collection and processing. A simple, sensitive and specific reversed-phase high-performance liquid chromatography (RP-HPLC) method was developed and validated for the rapid assay of salicin, which was aimed at distinguishing poplar tree gum from propolis. Isocratic elution at a flow rate of 1.0 mL/min was employed on a Sepax HP-C18 column (150 × 4.6 mm, 5 μm) and the column temperature was 30 °C. The mobile phase consisted of acetonitrile and 0.5% aqueous phosphoric acid (5:95, v/v). The UV detection wavelength was 213 nm. Following this method, salicin was detected in populus buds and leaves and 11 poplar tree gum samples, but not in any of the 40 propolis samples, which indicates that salicin was hydrolysed in propolis collection and processing but was stable in the production process of poplar tree gum. The proposed method could be an effective technique for routine analysis of salicin and monitoring the quality of propolis as possible counterfeit poplar tree gum.     

In vivo antitumoural activity and composition of an oil extract of Brazilian propolis

The present study aimed to evaluate in vivo and in vitro the antitumoural activity of a propolis extract obtained with edible vegetable oil and its fractions and also to investigate its chemical composition by LC–MS and LC–MS/MS. To evaluate the toxicological aspects related to the propolis extract treatment, hematological, biochemical, histopathological and morphological analyses of treated animals were performed. All propolis extracts showed an in vivo antitumour activity in the experimental model with a moderate toxicity effect at experimental exposure levels. The oil extract was as effective as the ethanolic extract at inhibiting tumour growth. In vitro assays showed that the whole oil extract produced better inhibition of tumour cells than its fractions. LC–MS and LC–MS/MS identified four phenolic acids and three flavonoids. The anticancer potential of the oil extract of propolis has been demonstrated and the edible vegetable oil was shown as an attractive alternative solvent to extract bioactive natural propolis components.     

Bioassay-guided isolation and identification of antifungal components from propolis against Penicillium italicum

The present study was aimed at identification of antifungal components against Penicillium italicum from Chinese propolis with bioassay-guided fractionation technique. Propolis ethanolic extract (PEE) was separated and purified by liquid–liquid extraction and thin layer chromatography (TLC) and the most active band was subjected to HPLC–MS/MS to identify the antifungal compounds. The results showed PEE and its fractions had strong antifungal activity against P. italicum. Among the fractions of PEE partitioned by petroleum ether, ethyl acetate, n-butanol and water, ethyl acetate fraction (E-Fr) exhibited the most effective activity against P. italicum. Further bioautographic TLC assay showed Band I, with Rf value of 0.70, had an inhibitive zone, which showed the strongest antifungal activity and completely inhibited the growth of P.italicum at 200 mg/L. Bioactive components found in Band I were further identified as pinobanksin, pinocembrine, chrysin and galangin. This study exhibited Chinese propolis and its main flavonoids was potential natural alternatives for the control of citrus blue mould caused by P.italicum.     

利用HPLC测定蜂胶中阿魏酸含量

对三种不同的蜂胶制品用反相高效液相色谱法测定了其中阿魏酸的含量 ,色谱柱为Nova -PakC18柱 ,3.9×15 0mm ;流速为 0 .8mL/min ;检测器为UV340nm× 0 .0 1AUFS。该实验希望对蜂胶制品的质量标准的制定有所帮助     

Propolis to Curb Lifestyle Related Disorders: An Overview

The natural products are gaining immense importance in the domain of nutrition to prevent various maladies and improve the quality of life. Among these, natural exudates are of significant worth as these biochemical compounds are released by various living entities having pharmacological properties for utilization in various drug developments. These natural exudates are the promising source for the discovery of new medications. Numerous bioactive moieties collected by honeybees from exudates and buds of particular trees and plants, considered to be utilized as defensive barrier with special reference to propolis. It generally contains numerous biochemical components, i.e., polyphenols, steroids, terpenoids, and amino acids. They also contain isoferulic acid, sinapinic acid, caffeic acid, and chrysin responsible for antibacterial perspectives. With special attention to propolis, it has been utilized in folk medicines due to several of its therapeutic activities, i.e., antioxidant, antidiabetic, anti-inflammatory, antiviral, and anticancer properties. In this context, it is extensively used in foodstuffs and beverages to improve health related disorders like inflammation, diabetes, heart disease, protects injured gums, and cancer insurgence. Moreover, it has been used to curtail stomatology, gastroenterology, skin lesions, and otorhinolaryngologic and respiration diseases.