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排序方式: 共有1134条查询结果,搜索用时 31 毫秒
1.
Yaxin Chen Jiexiang Tong Jiahao Dong Jing Luo Xiaoya Liu 《Small (Weinheim an der Bergstrasse, Germany)》2019,15(13)
Fast and highly efficient enrichment and separation of glycoproteins is essential in many biological applications, but the lack of materials with high capture capacity, fast, and efficient enrichment/separation makes it a challenge. Here, a temperature‐responsive core cross‐linked star (CCS) polymer with boronate affinity is reported for fast and efficient enriching and separating of glycoproteins from biological samples. The temperature‐responsive CCS polymers containing boronic acid in its polymeric arms and poly(N‐isopropyl acrylamide) in its cross‐linked core are prepared using reversible addition‐fragmentation chain transfer polymerization via an “arm‐first” methodology. The soluble boronate polymeric arms of the CCS polymers provide a homogeneous reaction system and facilitate interactions between boronic acid and glycoproteins, which leads to a fast binding/desorption speed and high capture capacity. Maximum binding capacity of the prepared CCS polymer for horseradish peroxidase is determined to be 210 mg g?1, which can be achieved within 20 min. More interestingly, the temperature‐responsive CCS polymers exhibit rapid reversible thermal‐induced volume phase transition by increasing the temperature from 15 to 30 °C, resulting in a facile and convenient sample collection and recovery for the target glycoproteins. Finally, the temperature‐responsive CCS polymer is successfully applied to enrichment of low abundant glycoproteins. 相似文献
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根据星轮装载机构的工作运动特点及掘进机的工作性能,通过对星轮装载机构的力学分析,介绍了星轮装载机构的星轮结构、星轮转速及装载功率的确定方法,为研究设计提供理论依据,以供参考。 相似文献
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多TDI CCD拼接相机在异速工作模式下,不同通道的CCD图像间会存在相互干扰现象,不同行频差会在图像上产生不同斜率、不同宽度的干扰斜条纹。为了解决此问题,文中对原设计电路系统进行了详细分析和优化设计。首先,从异速时序在不同行周期的相位差别上进行分析,异速模式下不同CCD成像电路时序间存在行频差异,导致各个行周期内CCD个别像元上与其他像元叠加的干扰不一致,是干扰斜条纹产生的原因。然后,采取优化关键信号布线方式、系统地和电源布置、去耦电容接地方式等多方面对电路系统进行了改进,优化多TDI CCD成像系统电路抗异速干扰设计。最后,对改进后的电路系统进行测试,去除干扰斜纹,并将图像均方根噪声水平由21.5 mV降低到4.2 mV。结果表明:采用抗干扰技术,有效地去除了通道间干扰斜纹,提高了图像质量。 相似文献
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Star‐shaped amphiphilic block polyurethane with pentaerythritol core for a hydrophobic drug delivery carrier 下载免费PDF全文
Shihai Wang Yu Zhou Bo Zhuang Peng Zheng Hongxiang Chen Tongcun Zhang Haiman Hu Dengcheng Huang 《Polymer International》2016,65(5):551-558
To obtain polyurethane micelles with excellent stability as a drug delivery carrier, star‐shaped amphiphilic block polyurethane (SAPU) was successfully synthesized by the ‘arm‐first’ method, using methoxypoly(ethylene glycol) and poly(ε‐caprolactone) diol as soft segments, hexamethylene diisocyanate as hard segments and pentaerythritol as the core. The structure of the SAPU was characterized by Fourier transform infrared spectroscopy, 1H NMR spectroscopy and gel permeation chromatography. The micellization behaviour and micelle properties of SAPU were measured by the pyrene fluorescence probe technique, 1H NMR, SEM and dynamic light scattering. The results indicated that SAPU could self‐assemble to form nanomicelles in aqueous solution and that the micelles showed excellent stability upon dilution and storage. Indometacin as a model drug could be incorporated into SAPU micelles and be released sustainedly. Meanwhile, the hydrophilic segment content and the molecular weight of SAPU had effects on the micelle properties. In addition, SAPU exhibited good cytocompatibility estimated by methylthiazole‐tetrazolium assay. © 2016 Society of Chemical Industry 相似文献
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《核技术(英文版)》2016,(4):109-117
We investigated the properties of strange quark matter in an external strong magnetic field with both confinement and leading-order perturbative interactions considered. It was found that the leading-order perturbative interaction can stiffen the equation of state of magnetized quark matter, while the magnetic field lowers the minimum energy per baryon. By solving the Tolman–Oppenheimer–Volkoff equations, we obtain the internal structure of strange stars. The maximum mass of strange stars can be as large as 2 times the solar mass. 相似文献
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This article presents a review of glyph-based techniques for engineering visualization as well as practical application for the multivariate visualization process. Two glyph techniques, Chernoff faces and star glyphs, uncommonly used in engineering practice, are described, applied to the selected data set, run through the chosen optimization methods and user evaluated. As an example of how these techniques function, a set of data for the optimization of a heat exchanger with a microchannel coil is adopted for visualization. The results acquired by the chosen visualization techniques are related to the results of optimization carried out by the response surface method and compared with the results of user evaluation. Based on the data set from engineering research and practice, the advantages and disadvantages of these techniques for engineering visualization are identified and discussed. 相似文献
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We present a quantum Monte Carlo application for the computation of energy eigenvalues for atoms and ions in strong magnetic fields. The required guiding wave functions are obtained with the Hartree–Fock–Roothaan code described in the accompanying publication (Schimeczek and Wunner, 2014). Our method yields highly accurate results for the binding energies of symmetry subspace ground states and at the same time provides a means for quantifying the quality of the results obtained with the above-mentioned Hartree–Fock–Roothaan method. 相似文献
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This article presents a scheme for improving the power output of grid-connected induction generator commonly used in wind energy conversion systems. Generally, the stator of the induction generator is connected in a star with a line voltage of √3 times the rated winding voltage to reduce the line current and, hence, conductor size. To extend the generating operation over a wider speed range, delta-star switchable stator windings are also in vogue. In such cases, the stator is star connected in the lower speed range and switched to a delta connection above a threshold speed. In this study, a new switching scheme is proposed wherein the stator coils are always connected in a star, while the stator is connected to different voltages in low- and high-speed conditions. At low wind speeds, nominal winding voltage is applied to the stator, whereas at higher speeds, the stator applied voltage is √3 times higher than the rated winding voltage. The efficacy of the scheme is demonstrated experimentally with a suitable microcontroller-based switching arrangement. Typical results indicate an increase in output with reduced switching transients. A case study on a 3-Φ, 50-kW induction generator is presented to emphasize the performance improvement with the proposed scheme. 相似文献