Sub‐100‐nanometre resolution in total internal reflection fluorescence microscopy

Combining total internal reflection fluorescence microscopy with structured illumination allows optical wide‐field imaging with sub‐100‐nanometre resolution. We present a novel objective‐launch set‐up for standing wave illumination that takes advantage of a tunable transmission diffraction grating and transparent phase shifters actuated by electro‐active polymers to control the excitation pattern in three dimensions. Image acquisition is completed in less than 1 s. To reconstruct the extended image spectrum, we apply a new apodization function that results in a lateral resolution of 89 nm for green emission wavelength.     

Mathematical Knowledge Management in HELM

The paper describes the general philosophy and the main architectural and technological solutions adopted in the HELM Project for the management of large repositories of mathematical knowledge. The leitmotiv is the extensive use of XML technology, and the exploitation of information in the Web way, that is without a central authority, with few basic rules, in a scalable, adaptable, and extensible manner.     

基于分层极限学习机和局部稀疏模型的视觉跟踪算法*

目标跟踪过程中常遇到形变和部分遮挡问题.为了解决该问题,文中提出结合分层极限学习机和自适应结构化局部稀疏外貌模型的视觉跟踪算法.分层极限学习机具有提取鲁棒特征并快速分类的能力.自适应结构化局部稀疏外貌模型可以使跟踪结果更准确,也可以解决部分遮挡问题.在不同视频序列上的性能测试表明文中算法在保持较高跟踪精度的同时,跟踪过程也较稳定.