| 具有抑菌作用乳酸菌的筛选及其抑菌物质特性的研究 |
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| 引用本文： | 于娜,陈忠军.具有抑菌作用乳酸菌的筛选及其抑菌物质特性的研究[J].食品科技,2011(5):13-17. |
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| 作者姓名： | 于娜 陈忠军 |
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| 作者单位： | 内蒙古农业大学食品科学与工程学院; |
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| 摘 要： | 从内蒙古地区传统谷物发酵食品——酸粥中分离并鉴定的13株乳酸杆菌中筛选具有抑菌活性的菌株,并对其抑菌能力及抑菌物质的特性进行研究。结果表明,菌株NSZL-2(鼠李糖乳杆菌L.rhamnose)和NSZL-12(植物乳杆菌L.plantarum)对指示菌具有较高的抑菌活性,且抑菌能力主要来自菌体的发酵代谢产物。排除酸和过氧化氢的干扰后代谢产物仍具有抑菌活性,且抑菌物质对热具有很高的稳定性。代谢产物经蛋白酶K、胃蛋白酶、胰蛋白酶、木瓜蛋白酶、过氧化氢酶、溶菌酶处理后,抑菌能力均有不同程度的降低,说明抑菌物质对蛋白酶敏感,故判定抑菌物质中有蛋白质类细菌素。
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| 关 键 词： | 乳杆菌 抑菌物质 细菌素 |
Screening of lactic acid bacteria for bacteriostatic activity and characteristics of antibacterial compounds |
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| YU Na,CHEN Zhong-jun.Screening of lactic acid bacteria for bacteriostatic activity and characteristics of antibacterial compounds[J].Food Science and Technology,2011(5):13-17. |
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| Authors: | YU Na CHEN Zhong-jun |
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| Affiliation: | YU Na,CHEN Zhong-jun* (College of Food Science and Engineering,Inner Mongolia Agricultural University,Huhhot 010018) |
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| Abstract: | the antibacterial capacities of 13 strains Lactobacillus which were isolated from traditional grain fermentation food-acid gruel in Inner Mongolia were studied. The result showed that the metabolins of NSZL-2(Lactobacillus. rhamnosus)and NSZL-12(Lactobacillus plantarum)were of obvious antibacterial activity and heat stability. The metabolins still revealed inhibited activity after excluded acid and hydrogen peroxide, meanwhile were sensitive to proteinase. It expressed that there was bacteriocin in inhibito... |
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| Keywords: | lactic acid bacteria antibacterial substance bacteriocin |
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