| HPLC分离黄曲霉毒素生防微生物的拮抗物及拮抗效果检测 |
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| 引用本文： | 王凯,闫培生,王琢,任秀莲,郇征宇.HPLC分离黄曲霉毒素生防微生物的拮抗物及拮抗效果检测[J].食品科技,2011(8):32-34. |
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| 作者姓名： | 王凯 闫培生 王琢 任秀莲 郇征宇 |
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| 作者单位： | 哈尔滨工业大学(威海)海洋学院;哈尔滨工业大学市政环境工程学院; |
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| 基金项目： | 国家自然科学基金项目(30571244,30870003); 哈尔滨工业大学科研创新基金项目(IMXK57080022) |
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| 摘 要： | 黄曲霉毒素在世界范围内不仅带来巨大的经济损失,而且严重威胁人们的身体健康。生物防治技术在黄曲霉毒素防治方面具有一定优势。利用课题组从土壤中筛选出的一株黄曲霉毒素生防细菌B05,使用HPLC对其代谢产物进行初步分离,确定了分离条件。将分离物收集后通过微孔板培养,确定了各分离组分对黄曲霉毒素的拮抗效果。
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| 关 键 词： | 黄曲霉毒素 生物防治 拮抗物 HPLC 线性梯度洗脱 |
Purify the antagonists of biological-control agent against aflatoxin by HPLC and detect their inhibitive effect |
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| WANG Kai,YAN Pei-sheng,WANG Zhuo,REN Xiu-lian,HUAN Zheng-yu.Purify the antagonists of biological-control agent against aflatoxin by HPLC and detect their inhibitive effect[J].Food Science and Technology,2011(8):32-34. |
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| Authors: | WANG Kai YAN Pei-sheng WANG Zhuo REN Xiu-lian HUAN Zheng-yu |
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| Affiliation: | WANG Kai1,2,YAN Pei-sheng1*,WANG Zhuo2,REN Xiu-lian1,HUAN Zheng-yu1(1.School of the Ocean,Harbin Institute of Technology at Weihai,Weihai 264209,2.School of Municipal & Environmental Engineering,Harbin Institute of Technology,Harbin 150090) |
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| Abstract: | In the world aflatoxins lead to great economy loss and seriously endanger human health.The biological-control method has advantage on inhibiting aflatoxins.B05 isolated from soil was used as biological-control agent against aflatoxins.The antagonists of biological-control agent was purified by using HPLC and the purification condition was confirmed.The isolates were collected and their inhibitive effect was detected by microtiter agar plate assay. |
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| Keywords: | aflatoxin biological-control antagonist HPLC gradient elution |
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