|
|||||
|
|
| C.pasteurianum高产1,3-丙二醇菌株的选育及发酵培养基的研究(Ⅰ) | |
| 引用本文： | 迟乃玉,张庆芳,王圆,李晓艳,李爱民,谷瑛,刘长江.C.pasteurianum高产1,3-丙二醇菌株的选育及发酵培养基的研究(Ⅰ)[J].食品与发酵工业,2003,29(6):10-13. |
| 作者姓名： | 迟乃玉 张庆芳 王圆 李晓艳 李爱民 谷瑛 刘长江 |
| 作者单位： | 1. 大连大学生物工程学院,大连,116622;大连大学生命科学工作室,大连,116622 2. 大连大学生物工程学院,大连,116622 3. 沈阳农业大学食品学院,沈阳,110161 |
| 摘 要： | 以淡水湖泊泥土中分离出的 13株梭菌 (Clostridium)为出发菌株 ,利用常规筛选方法选出 2株 1,3 丙二醇产生菌 (Clostridiumpasteurianum)。经UV、DES、NTG、EMS、LiCl单独及复合诱变 ,选育出一株 (CpN 38) 1,3 PD高产突变株。该突变株较出发菌株 ( 98- 77,98- 10 8) 1,3 PD产量提高了 7倍 ,产量为 31.0 0g/L。通过单因素实验 ,确定了CpN 38发酵培养基为 :甘油 ,90g/L ;NH4Cl,1.70g/L ;Fe2 +,0 .0 0 5 % ;Co2 +,0 .0 0 4 %。 |
| 关 键 词： | Clostridium pasteurianum 1 3-丙二醇 培养基 选育 |
| 修稿时间： | 2003年1月19日 |
The Screening of the 1,3-PD High-production Strain from Clostriduims and Studies on Its Fermentation Medium |
|
| Chi Naiyu , Zhang Qingfang Wang Yuan , Li Xiaoyan Li Aimin Gu Ying Liu Changjiang.The Screening of the 1,3-PD High-production Strain from Clostriduims and Studies on Its Fermentation Medium[J].Food and Fermentation Industries,2003,29(6):10-13. | |
| Authors: | Chi Naiyu Zhang Qingfang Wang Yuan Li Xiaoyan Li Aimin Gu Ying Liu Changjiang |
| Affiliation: | Chi Naiyu 1,3 Zhang Qingfang 1 Wang Yuan 1,3 Li Xiaoyan 1 Li Aimin 2 Gu Ying 2 Liu Changjiang 2 1 |
| Abstract: | Two 1,3 PD producing strains were obtained from 13 Clostridiums, which were isolated from freshwater marsh. A 1,3 PD producing mutant(CpN 38)was bred through multiple mutagenesis (UV, DES, NTG, EMS, LiCl etc).The yield of 1,3 PD was 31.00*#g/L, which was 7 times of original strain (Clostridium pasteurianum 98-107 and 99-77). Through monofactorial experiment, the optimal medium was obtained for producing 1,3 PD of CpN 38.The medium was 90*#g/L glycerol, 1.70*#g/L NH 4Cl, Fe 2+ 0.005%, Co 2+ 0.004 %. |
| Keywords: | Clostridium pasteurianum 1 3 PD medium screening |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |