| 应用响应面法优化超声波法提取甘草中总黄酮的工艺 |
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| 引用本文： | 逯家辉,姜鑫,李昊龙,郭伟良,刘艳,滕利荣.应用响应面法优化超声波法提取甘草中总黄酮的工艺[J].吉林大学学报(工学版),2008(Z2). |
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| 作者姓名： | 逯家辉 姜鑫 李昊龙 郭伟良 刘艳 滕利荣 |
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| 作者单位： | 吉林大学生命科学学院; |
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| 基金项目： | 吉林大学生物实验教学示范中心创新基金二类项目(ZXCX 2006011)． |
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| 摘 要： | 采用响应面法优化了超声波法提取甘草中总黄酮的条件。在单因素实验基础上,选择提取溶剂中乙醇体积分数、提取时间、超声波功率和液料比为随机因子,进行4因素3水平Box-Behnken中心组合设计,采用响应面法(RSM)分析了4个因素对响应值(总黄酮得率)的影响。实验结果表明,超声波法提取甘草中总黄酮的最优条件如下:乙醇体积分数为70.5%,超声波时间为20 min,超声波功率为328 W,液料比(mL:g)为20.8:1。在最优的条件下进行了9次验证实验,总黄酮的平均得率为4.31%,与理论值4.21%的相对误差为2.4%,理论值与实验值相吻合,说明该优化方法可行。
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| 关 键 词： | 生物化学工程 甘草 总黄酮 超声波提取法 响应面优化法 |
Optimization of ultrasonic-assisted extraction of general flavonoids in glycyrrhiza uralensis using the response surface method |
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| LU Jia-hui JIANG Xin LI Hao-long GUO Wei-liang LIU Yan TENG Li-rong.Optimization of ultrasonic-assisted extraction of general flavonoids in glycyrrhiza uralensis using the response surface method[J].Journal of Jilin University:Eng and Technol Ed,2008(Z2). |
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| Authors: | LU Jia-hui JIANG Xin LI Hao-long GUO Wei-liang LIU Yan TENG Li-rong |
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| Affiliation: | College of Life Science;Jilin University;Changchun 130012;China |
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| Abstract: | Response surface analysis methodology(RSM)was applied to optimize the ultrasonic- assisted extraction conditions of general flavonoids from glycyrrhiza uralensis.Based on single-factor experiments,four-factors-three-levels Box-behnken central composition experiments design were applied.The concentrations of ethanol in solvent,extraction time,ultrasonic power and ratio of liquid to solid were chosen as causal factors.RSM was employed to study the effect of these factors on the yield of general flavonoids fro... |
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| Keywords: | biochemical engineering glycyrrhiza uralensis general flavones ultrasound assisted extraction response surface analysis methodology |
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