当归润肠茶润肠通便功能和急性毒性研究

本文采用冰水混合物(0~2℃)灌胃建立脾胃虚寒型小鼠便秘模型,用150 mg/kg甲状腺素和2.0 mg/kg氢化可的松联合建立阴虚血瘀型小鼠便秘模型,用10 mg/kg复方地芬诺酯建立慢传输型小鼠便秘模型,研究当归润肠茶对模型小鼠首次排便时间、6h内排便量、粪便含水量和肠推进率的影响,用最大给药量评价当归润肠茶的急性毒性。结果发现,当归润肠茶高、中剂量对脾胃虚寒型便秘小鼠的排便时间显著缩短、排便量和肠推进率显著增加,当归润肠茶对阴虚血瘀型模型小鼠的排便时间显著缩短,当归润肠茶高剂量对阴虚血瘀型模型小鼠的排便量、粪便含水量和肠推进率均显著增加;当归润肠茶对慢传输型便秘小鼠首次排便时间均显著缩短、排便量显著增多,粪便含水量均显著增加、肠推进率均显著升高;按最高浓度最大给药容量灌胃后,当归润肠茶对小鼠未见明显急性毒性反应。说明当归润肠茶具有一定的润肠通便功能,口服安全性较高。

Laxative Function and Acute Toxicity of Danggui Runchang Tea

Three constipation models of spleen and stomach deficiency (SSD) mice (established by ice-water mixture (0~2 oC), yin deficiency and blood stasis (YDBS) mice (established by 2.0 mg/kg hydrocortisone and 150 mg/kg thyroxine ) and gastric slow transit constipation (GSTC) mice (established by 10 mg/kg compound diphenoxylate) were used to investigate the effects of Danggui Runchang tea (DRT) on the first defecation time, defecation within 6 h, fecal water content and peristalic rate of intestinal , and the acute toxicity of DRT was evaluated with maximum tolerance dose. The results showed that the high or medium dose of DRT significantly reduced the first defecation time, and the defecation and peristalic rate of intestinal was increased significantly in SSD mice. For YDBS mice, DRT significantly shortened the first defecation time, and high dose of DRT significantly increased the defecation, fecal water content and peristalic rate of intestinal. In addition, DRT reduced the first defecation time, and increased the defecation, fecal water content and peristalic rate of intestinal significantly in GSTC mice. No acute toxicity reaction was observed on mice by gavage at the highest concentration and maximum administration capacity, which showed that DRT had the laxative function and high oral administration safety.