| Application of N-succinimidyl 4-[(18)~F](fluoromethyl) benzoate to protein labeling |
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| 引用本文： | LI Jun-Ling,WANG Yong-Xian,ZHANG Xiu-Li,ZHOU Wei,TIAN Hai-Bin,YIN Duan-Zhi (Shanghai Institue of Nuclear Research,the Chinese Academy of Sciences,Shanghai 201800). Application of N-succinimidyl 4-[(18)~F](fluoromethyl) benzoate to protein labeling[J]. 核技术(英文版), 2003, 14(1) |
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| 作者姓名： | LI Jun-Ling WANG Yong-Xian ZHANG Xiu-Li ZHOU Wei TIAN Hai-Bin YIN Duan-Zhi (Shanghai Institue of Nuclear Research the Chinese Academy of Sciences Shanghai 201800) |
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| 作者单位： | Shanghai Institue of Nuclear Research，the Chinese Academy of Sciences，Shanghai 201800 |
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| 摘 要： | N-succinimidyl 4-[18F](fluoromethyl) benzoate for protein labeling was prepared (57%, EOB) in about 30min. Reaction conditions of S18FMB with IgG including pH of solutions, protein concentration, reaction temperature and time were studied. The optimal labeling conditions were: 0.2mg/mL IgG, pH = 7.8-8.5, 25℃, and reaction time 5min.Under these conditions the yield was about 80%. The 18F-labeled protein was purified by size exclusion chromatography.
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Application of N-succinimidyl 4-[18F](fluoromethyl) benzoate to protein labeling |
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| LI Jun-Ling,WANG Yong-xian,ZHANG Xiu-Li,ZHOU Wei,TIAN Hai-Bin,YIN Duan-Zhi. Application of N-succinimidyl 4-[18F](fluoromethyl) benzoate to protein labeling[J]. , 2003, 14(1) |
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| Authors: | LI Jun-Ling WANG Yong-xian ZHANG Xiu-Li ZHOU Wei TIAN Hai-Bin YIN Duan-Zhi |
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| Abstract: | N-succinimidyl 4-[18F](fluoromethyl) benzoate for protein labeling was prepared (57%, EOB) in about 30min. Reaction conditions of S18FMB with IgG including pH of solutions, protein concentration, reaction temperature and time were studied. The optimal labeling conditions were: 0.2mg/mL IgG, pH = 7.8-8.5, 25℃, and reaction time 5min.Under these conditions the yield was about 80%. The 18F-labeled protein was purified by size exclusion chromatography. |
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| Keywords: | S(18)~FMB IgG Protein labeling Size exclusion chromatography |
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