| 麻疯树籽粕中毒蛋白的提取 |
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| 引用本文： | 章宇宁,陈福明,张博宇,汪爱国,杨海燕. 麻疯树籽粕中毒蛋白的提取[J]. 北京服装学院学报(自然科学版), 2010, 30(4): 47-54. DOI: 10.3969/j.issn.1001-0564.2010.04.007 |
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| 作者姓名： | 章宇宁 陈福明 张博宇 汪爱国 杨海燕 |
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| 作者单位： | 深圳清华大学研究院,深圳,518057;深圳清华大学研究院,深圳,518057;深圳清华大学研究院,深圳,518057;深圳清华大学研究院,深圳,518057;深圳清华大学研究院,深圳,518057 |
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| 摘 要： | 研究了溶剂浸提法提取麻疯树籽饼粕中麻疯树毒蛋白时的各影响因素,考察了提取时间、提取温度、pH值、缓冲液浓度和无机盐浓度对麻疯树毒蛋白溶出的影响.结果表明:提取液pH值是影响麻疯树毒蛋白溶出的最重要因素,提取时间、缓冲液浓度及盐浓度对提取也有一定影响.通过正交试验获得最佳提取条件:提取时间3 h,提取温度25℃,提取液为浓度0.4 mol/L的NaCl溶液,pH=7.在此条件下得到的毒蛋白占去壳种仁的质量分数的0.39%.
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| 关 键 词： | 麻疯树籽 麻疯树毒蛋白 提取 |
Extraction of Curcin from Jatropha Curcas L.Seed Cake |
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| ZHANG Yu-ning,CHEN Fu-ming,ZHANG Bo-yu,WANG Ai-guo,YANG Hai-yan. Extraction of Curcin from Jatropha Curcas L.Seed Cake[J]. Journal of Beijing Institute of Clothing Technology, 2010, 30(4): 47-54. DOI: 10.3969/j.issn.1001-0564.2010.04.007 |
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| Authors: | ZHANG Yu-ning CHEN Fu-ming ZHANG Bo-yu WANG Ai-guo YANG Hai-yan |
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| Affiliation: | ZHANG Yu-ning,CHEN Fu-ming,ZHANG Bo-yu,WANG Ai-guo,YANG Hai-yan(Research Institute of Tsinghua University in Shenzhen,Shenzhen 518057,China) |
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| Abstract: | This paper studied the impact of extraction time, extraction temperature, pH, buffer concentration and inorganic salts concentration on solvent extraction of Curcin from Jatropha Curcas L. seed cake. The results showed that pH value was the most important factor, extraction time, buffer concentration and salt concentration also had an impact. The optimum extraction conditions are as the following： the extraction time is 3 h, the extraction temperature is 25 ℃, the concentration of NaC1 solution is 0.7 mol/L and the pH value is 7. Under the conditions the mass of curcin accounted for 0.39% of seeds （without shell）. |
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| Keywords: | Jatropha Curcas L. seed cake curcin extraction |
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