| 纳米细菌纤维素柱层析分离 |
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| 引用本文： | 吴周新,牛成,王桂振,张军峰,冯玉红,李开绵.纳米细菌纤维素柱层析分离[J].功能材料,2011(S5). |
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| 作者姓名： | 吴周新 牛成 王桂振 张军峰 冯玉红 李开绵 |
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| 作者单位： | 海南大学材料化工学院海南优势资源化工材料应用技术教育部重点实验室;中国热带农业科学院热带作物品种资源研究所; |
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| 基金项目： | 国家自然科学基金资助项目(50763002); 中央级公益性科研院所基本科研业务费专项资金资助项目(PZS054) |
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| 摘 要： | 以海南特有的椰子水为原料,通过木醋杆菌合成细菌纤维素(bacterial cellulose,简称BC)。通过膜分离将大分子凝胶和菌种以及破碎细胞除去,采用等电点法和Sevage法除蛋白、乙醚除脂肪、离子交换树脂除盐、DEAE-32柱进行柱层析得到中性纳米细菌纤维素,经过Sephadex-G100柱层析精制得到重均分子量(Mw)为9598,分子量分布指数约为1.1的纳米细菌纤维素。用透射电镜测试了其形貌,证明其颗粒近似球形,直径约20nm。
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| 关 键 词： | 纳米细菌纤维素 柱层析 DEAE-32柱 Sephadex-G100柱 |
Column chromatographie separation of nano bacterial cellulose |
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| WU Zhou-xin,NIU Cheng,WANG Gui-zhen,ZHANG Jun-feng,FENG Yu-hong,LI Kai-mian.Column chromatographie separation of nano bacterial cellulose[J].Journal of Functional Materials,2011(S5). |
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| Authors: | WU Zhou-xin NIU Cheng WANG Gui-zhen ZHANG Jun-feng FENG Yu-hong LI Kai-mian |
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| Affiliation: | WU Zhou-xin1,NIU Cheng1,WANG Gui-zhen1,ZHANG Jun-feng1,FENG Yu-hong1,LI Kai-mian2(1.Key Laboratory of Ministry of Education for Application Technology of Chemical Materials in Hainan SuperiorResources,College of Materials and Chemical Engineering,Hainan University,Haikou 570228,China,2.Tropical Crops Genetic Resource Institute,Chinese Academy of Tropical Agricultural Sciences,Danzhou 571737,China) |
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| Abstract: | In present study,nano bacterial cellulose(NBC) was produced with Hainan coconut juice as the raw material by Acetobacter xylinum HN001.Membrane separations technology was employed to wipe off macromolecule hydrogels,strains and smudge cells.Then neutral NBC were obtained using Isoionic point and Sevage removing albumen,aether removing fat,ion exchange resin eliminating celite and DEAE-32 cellulose anion exchange chromatography.Finally,the NBC with weight-average molar mass(Mw) 9598 and polydispersity index ... |
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| Keywords: | nano bacterial cellulose column cromatographie DEAE-32 cllulose column sephadex-G100 column |
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