| 免疫磁性微球的制备及其在丙肝病毒检测中的应用 |
| |
| 引用本文： | 杨明珠,官月平,赖仙红,谢立,侯涛,王强.免疫磁性微球的制备及其在丙肝病毒检测中的应用[J].功能材料,2011,42(10). |
| |
| 作者姓名： | 杨明珠 官月平 赖仙红 谢立 侯涛 王强 |
| |
| 作者单位： | 1. 北京科技大学材料科学与工程学院,北京,100083
2. 北京市卫生局肝炎研究所,北京,100054 |
| |
| 基金项目： | 国家高技术研究发展计划(863计划)资助项目(2007AA03Z315) |
| |
| 摘 要： | 采用悬浮-乳液聚合法合成了超顺磁性聚丙烯酸甲酯微球,通过氨解反应在微球表面修饰了氨基并偶联了丙肝抗体,通过硅烷化反应在载玻片上修饰了氨基并偶联了丙肝抗体。采用振动样品磁强计（VSM）、生物显微镜和原子吸收光谱仪（AAS）对合成的磁性微球进行了表征。结果表明微球尺寸均一,单分散性较好,平均粒径为4μm,比饱和磁化强度为7.181A.m2/kg,微球表面氨基浓度为0.433mmol/g,具有超顺磁性。通过微球表面的抗体和载玻片上的抗体与丙肝抗原的免疫反应检测丙肝病毒,阴、阳性得到了很好的区分,达到了较好的检测效果。
|
| 关 键 词： | 聚丙烯酸甲酯微球 免疫检测 悬浮-乳液聚合 |
Synthesis of immunomagnetic microspheres and their applications in the detection of hepatitis C virus antigen |
| |
| YANG Ming-zhu,GUAN Yue-ping,LAI Xian-hong,XIE Li,HOU Tao,WANG Qiang.Synthesis of immunomagnetic microspheres and their applications in the detection of hepatitis C virus antigen[J].Journal of Functional Materials,2011,42(10). |
| |
| Authors: | YANG Ming-zhu GUAN Yue-ping LAI Xian-hong XIE Li HOU Tao WANG Qiang |
| |
| Affiliation: | YANG Ming-zhu1,GUAN Yue-ping1,LAI Xian-hong1,XIE Li2,HOU Tao1,WANG Qiang1(1.School of Materials Science and Engineering,University of Science and Technology Beijing,Beijing 100083,China,2.Beijing Municipal Hepatitis Institute,Beijing 100054,China) |
| |
| Abstract: | Superparamagnetic poly(methyl acrylate) microspheres were synthesized by suspension-emulsion polymerization.Amino group was modified on the surface of the magnetic polymer and the slide by ammonolysis and silanized chemical reaction,respectively,then the hepatitis C virus antibody was immobilized.The obtained magnetic microspheres were characterized by means of vibrating sample magnetometry(VSM),biological microscope and atomic adsorption spectrophotometer(AAS).The results showed that the magnetic microsphe... |
| |
| Keywords: | magnetic poly(methyl acrylate) microspheres immunoassay suspension-emulsion polymerization |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
