Culture Medium Evaluation Using Low-Cost Substrate for Biosurfactants Lipopeptides Production by Bacillus amyloliquefaciens in Pilot Bioreactor |
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Authors: | Frederico A Lima Olga Silva Santos Alan William Vilela Pomella Eloízio J Ribeiro Miriam Maria de Resende |
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Affiliation: | 1. Chemical Engineering Faculty, Federal University of Uberlândia, P.O. Box 593, Av. João Naves de Ávila 2121, Campus Santa Mônica, Bloco 1K, Uberlândia, MG, 38408-144 Brazil;2. Laboratório de BioControle Farroupilha S.A., Lallemand, Av. Júlia Fernandes Caixeta n°55, Bairro Cidade Nova, Patos de Minas, MG, 38706-420 Brazil |
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Abstract: | Total biosurfactant production by Bacillus amyloliquefaciens IT45 was evaluated with different substrates concentrations in a culture medium. A central composite design (CCD) was developed to evaluate the influence of variables, including glucose syrup, yeast extract, and calcium chloride, on surface tension (ST), total biosurfactant production, and residual sugar (RS). As a result, the best observed results for ST, RS, and total biosurfactant production were 30 mN m?1, 31%, and 5.5 g L?1, respectively, after 48 h of fermentations carried out in batch operation process. Characterization of the biosurfactant identified the presence of surfactin. To validate the CCD experiments, fermentations were conducted in a 40 L pilot bioreactor. For this fermentation, the cellular growth was 3.0 × 109 CFU mL?1, surfactin production was 0.55 g L?1, and RS was 28%. The results demonstrate that B. amyloliquefaciens IT45 has the potential to produce biosurfactants and does not require high concentrations of carbon and nitrogen sources for its development. |
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Keywords: | Pilot bioreactor Surfactin production Value-aggregated bioproduct Bactericidal and antifungal function Process optimization |
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