| 高速逆流色谱法分离β-谷甾醇和菜油甾醇的研究 |
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| 引用本文： | 周玉杰,戴玲妹,陈福明,李总成.高速逆流色谱法分离β-谷甾醇和菜油甾醇的研究[J].精细化工,2002,19(3):183-185. |
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| 作者姓名： | 周玉杰 戴玲妹 陈福明 李总成 |
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| 作者单位： | 1. 清华大学化工系,北京,100084
2. 深圳清华大学研究院,广东,深圳,518057 |
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| 摘 要： | 利用高速逆流色谱法 ,并选用V(庚烷 )∶V(乙腈 )∶V(乙酸乙酯 ) =5∶5∶1溶剂体系 ,成功地分离了混合植物甾醇标准品中的 β 谷甾醇和菜油甾醇。经过HPLC检测 ,一次性分离可得到质量分数为 97%的 β 谷甾醇和质量分数为 91%的菜油甾醇产品 ,收率分别可达 5 6 %和 5 0 %。该方法简便、重现性好 ,可作为高纯度 β 谷甾醇和菜油甾醇标准品的制备分离方法
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| 关 键 词： | 高速逆流色谱 β谷甾醇 菜油甾醇 分离 提纯 |
| 文章编号： | 1003-5214(2002)03-0183-03 |
Separation of β-Sitosterol and Campesterol by High-speed Countercurrent Chromatography |
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| ZHOU Yu jie ,DAI Ling mei ,CHEN Fu ming ,LI Zong cheng.Separation of β-Sitosterol and Campesterol by High-speed Countercurrent Chromatography[J].Fine Chemicals,2002,19(3):183-185. |
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| Authors: | ZHOU Yu jie DAI Ling mei CHEN Fu ming LI Zong cheng |
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| Affiliation: | ZHOU Yu jie 1,DAI Ling mei 1,CHEN Fu ming 2,LI Zong cheng 2 |
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| Abstract: | β-Sitosterol and campesterol were successfully separated from mixed plant sterol standard by high speed countercurrent chromatography with V (heptane)∶ V (acetonitrile)∶ V (ethyl acetate)=5∶5∶1 solvent system.Monitored by HPLC,purities of the products could be as high as 97% for β sitosterol and 91% for campesterol,with 56% and 50% recovery respectively, through one step separation.This method is convenient and with good reproducibility and can be used as a preparative scale purification method for standards of the two phytosterols. |
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| Keywords: | high speed countercurrent chromatography (HSCCC) β sitosterol campesterol separation purification |
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