| 应用OPA柱前衍生法测定食品中的牛磺酸 |
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| 引用本文： | 任一平,黄百芬.应用OPA柱前衍生法测定食品中的牛磺酸[J].食品与发酵工业,1995(1):43-48. |
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| 作者姓名： | 任一平 黄百芬 |
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| 作者单位： | 浙江省轻工业研究所 |
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| 摘 要： | 本文研究了采用ＯＰＡ柱前衍生法测定食品中的牛磺酸。样品经粉碎、振荡、离心等前处理，通过ＯＤＳＣ－１８柱进行衍生和分离。柱前衍生剂为含有０．５ｇＯＰＡ和５ｍｌα－巯基乙醇的０．２ｍｏｌ／Ｌ（ｐＨ９．５）硼酸缓冲液。流动用为５５％的０．０５ｍｏｌ／Ｌ乙酸钠溶液和４５％的甲醇。荧光检测器波长为：Ｅｘ：３３５ｎｍ和ＥＭ：４５５ｎｍ．食品中牛磺酸浓度在５μｇ／１００ｇ范围内能够被定量测定。回收率为９９．６３～１００．０１％，变异系数（ＣＶ）为１．３８％。
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| 关 键 词： | 柱前衍生，邻苯二甲醛，牛磺酸 |
Application of Pre-Column Derivation to the Indentification of Taurine in Food |
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| Ren Yiping,Huang Baifen,Hu Hongwei.Application of Pre-Column Derivation to the Indentification of Taurine in Food[J].Food and Fermentation Industries,1995(1):43-48. |
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| Authors: | Ren Yiping Huang Baifen Hu Hongwei |
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| Abstract: | method for identification of taurine in foods by Pre-Column Derivation is described. The sample is pretreated simply through shivering,oscillating, centrifuging, derivation and separated by ODS C-18 column. The Pre-Column Derivation reagent is 0. 5g OPA and 5ml α-mercaptoethanol in 100ml 0.2mol/L boric acid buffer solution(pH= 9.5).The mobile phase is 55% 0. 05 mol/L sodium acetate and 45% methanol. The fluorescence detector wavelength is EX: 335nm and EM: 455nm. 5μg/100g taurine in food can be completely identified quantitatively. The recovery is from 99. 63 to 100. 01% .Coefficient of variation(CV) is 1. 38% . |
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| Keywords: | re-Column Derivation OPA Taurine |
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