菠萝蜜低聚肽的免疫调节作用研究

目的:探究菠萝蜜低聚肽(jackfruit oligopeptides,JOPs)免疫调节功能。方法:采用SPF级雌性BALB/c小鼠为实验对象,进行8项免疫实验测定,系统评价不同干预剂量(0.20、0.40、0.80 g/kg·bw)JOPs对小鼠免疫器官相对重量、细胞免疫功能、体液免疫功能、单核-巨噬细胞功能、自然杀伤细胞(nature killer cell,NK细胞)活性以及T淋巴细胞亚群的影响。结果:与空白对照组相比,JOPs 0.40、0.80 g/kg·bw剂量组脾脏指数显著提高(P<0.05),且JOPs 0.40 g/kg·bw剂量组脾脏指数显著高于乳清蛋白组(P<0.05);与空白对照组及乳清蛋白组相比,JOPs 0.40、0.80 g/kg·bw剂量组ConA诱导的小鼠脾淋巴细胞增殖能力、足跖增厚度、溶血空斑数、碳廓清吞噬指数a、巨噬细胞吞噬率与吞噬指数及NK细胞活性显著提高(P<0.05),JOPs 0.80 g/kg·bw剂量组血清溶血素含量、CD3+T细胞数量、CD4+T细胞百分比及CD4+CD25+/CD4+、CD4+/CD8+比值显著提高(P<0.05);各JOPs剂量组对小鼠体重增长、胸腺指数均无显著性影响(P>0.05)。结论:JOPs可提高机体适应性免疫及固有免疫功能,起到免疫增强作用。

Study on the Immunoregulatory Function of Jackfruit Oligopeptides

Objective:To investigate the immunomodulatory function of jackfruit oligopeptides(JOPs). Methods:SPF female BALB/c mice were used as experimental subjects,and 8 immunoassays were performed to systematically evaluate the effect of different intervention doses' JOPs(0.20,0.40,0.80 g/kg·bw)on relative weight of immune organ,relative weight,cellular immune function,humoral immune function,mononuclear-macrophage function,natural killer cell(NK cell)activity and the T lymphocyte subsets in mice. Results:Compared with the blank control group,the spleen index in the 0.40,0.80 g/kg·bw dose group of JOPs was significantly increased(P<0.05),and the spleen index of the JOPs 0.40 g/kg·bw dose group was significantly higher than that of the whey protein group(P<0.05). Compared with the blank control group as well as the whey protein group,the proliferation of spleen lymphocytes induced by ConA,the degree of footpad thickening,the number of hemolytic plaques,the carbon clearance phagocytosis index(a),The macrophage phagocytic rate as well as phagocytic index and NK cell activity of macrophages in the 0.40,0.80 g/kg·bw dose group of JOPs were significantly increased(P<0.05). Compared with the blank control group as well as the whey protein group,the serum hemolysin content,the number of CD3+T cells,the percentage of CD4+T cells and the ratio of CD4+CD25+/CD4+ and CD4+/CD8+ in the 0.80 g/kg·bw group of JOPs were significantly increased(P<0.05). There was no significant effect on the weight gain and thymus index of the mice in each dose group of JOPs(P>0.05). Conclusion:JOPs can strengthen the immune function by improving the function of body's adaptive and innate immunity.