| 双酶法提取甜瓜籽多肽的工艺研究 |
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| 引用本文： | 王思琪,傅晶依,丁修庆,刘佳鑫,赵珺.双酶法提取甜瓜籽多肽的工艺研究[J].食品研究与开发,2020,41(8):94-99. |
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| 作者姓名： | 王思琪 傅晶依 丁修庆 刘佳鑫 赵珺 |
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| 作者单位： | 长春大学食品科学与工程学院，吉林 长春 130022 |
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| 基金项目： | 长春大学与吉林大学联合培养“协同培养”项目 |
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| 摘 要： | 以甜瓜籽为原料,水解度为考察指标,采用胰蛋白酶和碱性蛋白酶双酶协同酶解法制备甜瓜籽多肽。通过单因素试验和响应面设计试验对酶解条件进行优化,结果表明:酶解的最佳工艺条件为底物浓度为3%、加酶量为4%、酶解时间为180 min、酶解温度为50℃、pH值为8、双酶质量比为6∶4,水解度达到最大值13.85%。
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| 关 键 词： | 甜瓜籽 多肽 双酶酶解 水解度 工艺优化 |
| 收稿时间： | 2020/1/31 0:00:00 |
Study on Extraction Technology of Melon Seed Peptides by Dual-enzymatic Hydrolysis |
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| WANG Si-qi,FU Jing-yi,DING Xiu-qing,LIU Jia-xin,ZHAO Jun.Study on Extraction Technology of Melon Seed Peptides by Dual-enzymatic Hydrolysis[J].Food Research and Developent,2020,41(8):94-99. |
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| Authors: | WANG Si-qi FU Jing-yi DING Xiu-qing LIU Jia-xin ZHAO Jun |
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| Affiliation: | (College of Food Science and Engineering,Changchun University,Changchun 130022,Jilin,China) |
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| Abstract: | Melon seeds was used as raw materials,and taking the degree of hydrolysis as an index,trypsin and alkaline protease were used to prepare the melon seed polypeptide.The single-factor test and response surface design test were used to optimize the enzymolysis conditions.The results showed that the best conditions for enzymolysis were found to be:substrate concentration 3%;enzyme amount 4%;hydrolysis time 180 min;hydrolysis temperature 50℃;pH 8 and dual-enzymatic mass ratio 6∶4.The degree of hydrolysis reached a maximum of 13.85%. |
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| Keywords: | melon seed polypeptide dual-enzymatic hydrolysis the degree of hydrolysis process optimization |
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