具有潜在改善炎症反应益生菌的筛选

目的:探讨供试菌株对脂多糖(LPS)诱导THP-1细胞分泌炎性细胞因子的调节作用,筛选出具有潜在改善炎症反应的益生菌。方法:通过佛波酯(PMA)诱导THP-1成为成熟巨噬细胞,脂多糖(LPS)刺激成熟巨噬细胞产生炎症反应,评价益生菌对这种炎症反应的改善作用,并对具有改善炎症反应潜力的益生菌株进行胃肠道耐受能力、粘附能力、抑菌能力及抗生素敏感性评价。结果:植物乳杆菌SS-9、SD-H9、鼠李糖乳杆菌SD-L8及罗伊氏乳杆菌WX-94具有改善机体炎症反应的潜力,其中SS-9和SD-H9能显著降低炎症模型细胞IL-1β的分泌(P<0.05),WX-94和SS-9能显著降低炎症模型细胞的IL-6的分泌(P<0.05);同时四株菌均具有较高的胃肠道耐受能力、粘附能力,具有安全通过肠道的潜力;并可以不同程度地抑制致病菌大肠杆菌、金黄色葡萄球菌、铜绿假单胞菌及阴沟肠杆菌的生长,具有抑制肠道感染的潜力;抗生素敏感性研究表明,四株菌均未发现抗生素耐受的风险。结论:植物乳杆菌SS-9、SD-H9、鼠李糖乳杆菌SD-L8及罗伊氏乳杆菌WX-94均可作为具有潜在炎症调节能力的益生菌进行开发和应用。

Screening of Probiotics with Potential to Improve Inflammatory Response

Objective:To explore the regulatory effects of the tested strains on the secretion of inflammatory cytokines in THP-1 cells induced by lipopolysaccharide(LPS),and to screen probiotics with the potential function of improving the inflammatory response. Methods:THP-1 was induced into mature macrophages by phorbol ester(PMA),then lipopolysaccharide(LPS)stimulated mature macrophages to produce inflammatory responses,so as to evaluate the improvement effect of probiotics on this inflammatory response. Besides,the gastrointestinal tolerance,adhesion,bacteriostatic ability and antimicrobial susceptibility of probiotic strains with the potential function of improving inflammatory response were evaluated. Results:Lactobacillus plantarum SS-9,Lactobacillus plantarum SD-H9,Lactobacillus rhamnosus SD-L8 and Lactobacillus reuteri WX-94 had the potential function to improve inflammatory response. SS-9 and SD-H9 could significantly reduce the secretion of IL-1β in inflammatory model cells(P<0.05). WX-94 and SS-9 could significantly reduce the secretion of IL-6(P<0.05). Four kinds of strains of bacteria had high gastrointestinal tolerance,adhesion ability,and had the potential to safely pass through the intestine. Four kinds of strains of bacteria could inhibit the growth of pathogenic bacteria such as Escherichia coli,Staphylococcus aureus,Pseudomonas aeruginosa and Enterobacter cloacae in varying degrees,and had the potential to inhibit intestinal infection. Results of antibiotic susceptibility showed that none of the four kinds of strains had a risk of antibiotic tolerance. Conclusion:Lactobacillus plantarum SS-9,Lactobacillus plantarum SD-H9,Lactobacillus rhamnosus SD-L8 and Lactobacillus reuteri WX-94 can be developed and applied as probiotics with potential inflammatory regulation functions.