胶基型嚼烟中19种多环芳烃的气相色谱—串联质谱技术法测定

建立了一种应用气相色谱—串联质谱技术(GC-MS/MS)测定胶基型嚼烟中19种多环芳烃含量的方法,内标法定量,分别对样品预处理方式和串联质谱检测条件进行了优化。结果表明:①在优化的试验条件下,19种多环芳烃的检出限、定量限分别可达0.04~0.14,0.14~0.46 ng/mL,线性相关系数达0.998以上;②高、中、低3个加标浓度样品回收率范围为92.26%~109.46%;③方法的日内精密度和日间精密度分别为0.77%~3.85%,1.57%~4.86%,均小于5.00%(n=6)。该方法灵敏度高、线性关系好,是胶基型嚼烟质量控制监控的有效手段之一。

Determination of polycyclic aromatic hydrocarbons in gum-based chewing tobacco by GC-MS/MS

In this study, an inter-standard quantitative method was used for determination of PAHs in gum-based chewing tobacco by GC-MS/MS. Under the control of the optimized experimental conditions, the LODs and the LOQs of 19 target PAHs could achieve 0.04~0.14 ng/mL and 0.14~0.46 ng/mL, respectively, while the linear correlation efficiencies were larger than 0.998 for all PAHs. With three different adding-levels, the recovery rates for all PAHs ranging from 92.26% to 109.46%. And the Intra-assay and Inter-assay precisions for the PAHs were 0.77%~3.85% and 1.57%~4.86%, respectively. The method developed was reliable and suitable for routine analysis of PAHs, and was verified to be one of the effective methods for quality control of gum-based chewing tobacco.