洛神花多糖对大鼠急性肝损伤干预作用

研究洛神花多糖提取物对Wister大鼠急性肝损伤模型的干预作用。首先采用乙醇灌胃法建立大鼠急性肝损伤模型,将成模大鼠分为模型组、实验组(洛神花多糖 1.0、2.0、4.0、8.0 g/kg 组)、阳性对照组("海王金樽"解酒药 10 mg/kg BW),以健康大鼠作为正常组,持续治疗7 d,颈动脉采血测定各组大鼠血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸脂酶(ALP)和胆碱酯酶(CHE)的活力及总蛋白(TP)、白蛋白(ALB)、球蛋白(GLB)和谷胱甘肽(GSH)的含量;称量各组大鼠肝脏重量,计算肝脏指数,以及肝脏组织切片的观察;对洛神花多糖提取物对急性肝损伤大鼠干预效果进行综合评价。结果表明,与模型组相比,4.0 g/kg洛神花治疗组血清中ALT、AST、ALP活力均极显著降低(P<0.01),TP、GLB含量显著降低(P<0.05),CHE活力显著增高(P<0.05),洛神花多糖提取物对ALD大鼠体重具有一定稳定作用,能有效减少胃黏膜组织溃疡、减轻肝脏肿胀与肝细胞变性坏死情况。因此洛神花多糖提取物对急性肝损伤大鼠具有良好的干预治疗作用和保肝能力,具有良好开发应用潜力。

Intervention of Roselle Flower Polysaccharide on Acute Liver Injury in Rats

Intervention effect of roselle polysaccharide extract on the model of acute liver injury in Wister rats was studied. The rat model of acute liver injury was established by the method of ethanol gavage.The model rats were divided into model group, treatment group (1.0, 2.0, 4.0, 8.0 g/kg roselle polysaccharide), positive group (Alcoholic medicine 10 mg/kg BW), while the healthy rats were as the normal group. All the groups were continues treated for 7 d, and the carotid blood was collected. The serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphate enzyme (ALP) and cholinesterase (CHE) activity and total protein (TP), albumin (ALB), globulin (GLB), and glutathione (GSH) contents were determined. The liver weight and liver index were calculated. And the section of liver tissue was observed. The effect of roselle polysaccharide extract on the intervention of rats with acute liver injury was comprehensively evaluated. The results showed that, compared with the model group, the serum ALT, AST, and ALP activities of 4.0 g/kg roselle treatment group extremely significantly reduced (P<0.01), and the TP and GLB contents significantly reduced (P<0.05), while the CHE activity significantly increased (P<0.05), which indicated that the roselle polysaccharide had a certain stabilizing effect on the body weight of ALD rats, and could effectively reduce gastric ulcers, liver swelling and hepatocyte degeneration and necrosis. The roselle polysaccharide extract has good interventional treatment and liver protection ability in rats with acute liver injury, and has good development and application potential.