黄花草总黄酮超声辅助提取工艺优化及抗氧化活性研究

采用超声辅助法提取黄花草总黄酮,通过单因素试验和正交试验确定了总黄酮的最佳提取工艺条件,并研究了黄花草总黄酮对羟基自由基(·OH)、DPPH自由基(DPPH·)和亚硝酸盐的清除效果。结果表明:黄花草总黄酮的最佳提取工艺条件为料液比1:15 (g/mL),乙醇浓度50%,提取功率40 W,超声时间50 min,提取温度50℃,该条件下黄花草总黄酮得率为(2.711±0.002)%。黄花草总黄酮对·OH和亚硝酸盐具有明显清除能力,对DPPH·具有较强清除能力,最大清除率分别为(52.48±0.88)%,(95.58±0.28)%,(57.27±0.15)%,表明黄花草中的总黄酮具有较好的抗氧化能力。

Ultrasonic-assisted extraction technology optimization and antioxidant activity of total flavonoids from Cleome viscosa

Total flavonoids were extracted fromCleome viscosa by ultrasound-assisted extraction. The optimum extraction conditions were investigated based on single factor and orthogonal tests.Meanwhile,the antioxidant activities of total flavonoids were evaluated by studying hydroxyl radical(·OH)scavenging assay,1,1-diphenyl-2-picrylhydrazyl free radical(DPPH · )scavenging assay and nitrite scavenging assay.The results showed that the extraction rate of total flavonoids of Cleome viscosa was(2.711±0.002)%under the following conditions:material-to-liquid ratio of 1:15(g/mL),ethanol concentration of 50%,extraction power of 40 W,ultrasonic time of 50 min,extraction temperature of 50 ℃.Total flavonoids of Cleome viscosa exhibited obvious · OH and nitrite scavenging capacity,and showed a higher scavenging capacity to DPPH·,and the corresponding maximum scavenging rates were(52.48±0.88)%,(95.58±0.28)% and(57.27±0.15)%,respectively.The results revealed that total flavonoids of Cleome viscosa had excellent antioxidant activities.