桃胶多糖体内外抗氧化作用的研究

为了研究桃胶多糖体内外抗氧化的作用,实验采用体外测定桃胶多糖对1,1-二苯基-2-三硝基苯肼自由基(DPPH·)、2,2'-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐阳离子自由基(ABTS+·)、羟基自由基(·OH)和超氧阴离子(O₂-·)等的清除率及对铁的还原能力,体内通过建立D-半乳糖小鼠氧化损伤模型,给予不同剂量(2、4、6 g/kg)桃胶多糖灌胃,测定血清及肝脏中丙二醛(MDA)含量及总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性。结果显示,桃胶多糖体外对DPPH·、ABTS+·、·OH和O₂-·的半数抑制浓度(IC₅₀)分别为6.95、0.56、1.10、0.11 mg/mL,且对铁还原力随着浓度(0.02~0.14 mg/mL)的升高而增大;在体内实验中,与模型组相比,连续服用不同剂量(2、4、6 g/kg)的桃胶多糖能够显著或极显著降低小鼠血清及肝脏中MDA含量、提高T-AOC能力、增强SOD及GSH-Px的活性(P<0.05或P<0.01),同时剂量间存在一定的量效关系。研究结果表明桃胶多糖具有良好的体内外抗氧化活性,可进一步申报成为新型的食品资源,应用于制药与食品工业中抗氧化、抗衰老、降血糖等保健产品的开发。

Antioxidant Activity in Vivo and in Vitro of Polysaccharide from Peach Gum

In order to study the antioxidative effect of peach gum polysaccharide in vitro and in vivo, this experiment determined the removal rates of 1, 1-diphenyl-2-trinitrophenylhydrazine free radicals (DPPH·), 2, 2-diazo-bis (3-ethyl-benzothiazo-6-sulfonic acid) diammonium salt free cation (ABTS+·), hydroxyl radical (·OH) and superoxide anion (O₂-·) and reduction ability of iron in vitro. An oxidative damage model of D-galactose mice was established, and different doses (2, 4, 6 g/kg) of polysaccharide of peach gum were injected into the stomach. The content of MDA and the activities of total antioxidant capacity (T-AOC), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activity in serum and liver were determined. The results showed that the 50% inhibition concentration (IC₅₀) of DPPH·, ABTS+·,·OH and O₂-·in vitro were 6.95, 0.56, 1.10, and 0.11 mg/mL, respectively. And the resistance to iron increased with the increase of the concentration (0.02~0.14 mg/mL).In vivo, compared with the model group, continuous administration of different doses (2, 4, 6 g/kg) of peach gum polysaccharide could significantly or extremely significantly reduce the MDA content in the serum and liver of mice, improve T-AOC ability, and enhance the activity of SOD and GSH-Px (P<0.05 or P<0.01). At the same time, there was a certain quantitative effect relationship between doses. The results show that polysaccharide from peach gum has good antioxidant activity in vivo and in vitro, and can be used as a new antioxidant and free radical scavenger.