暗纹东方鲀鱼精蛋白的纯化及抑菌活性研究

采用葡聚糖凝胶(Sephadex G-50)层析和羧甲基琼脂糖凝胶FF(CM Sepharose Fast Flow)离子交换层析对暗纹东方鲀鱼精蛋白进行纯化,利用精氨酸显色反应鉴定鱼精蛋白,并测定了鱼精蛋白对金黄色葡萄球菌、大肠杆菌和沙门氏菌的最小抑菌浓度、抑菌圈直径和生长曲线的影响。结果表明,暗纹东方鲀鱼精蛋白在217,273nm处各有1个吸收峰且217nm处的吸收峰较高,经SDS-PAGE电泳发现纯化后的鱼精蛋白白条带较纯化前的细且无拖尾现象;暗纹东方鲀鱼精蛋白对革兰氏阴性菌(沙门氏菌和大肠杆菌)和革兰氏阳性菌(金黄色葡萄球菌)均具有抑制作用,可以延缓菌种的生长,对金黄色葡萄球菌、大肠杆菌和沙门氏菌的最小抑菌浓度分别为500,1 000,1 000mg/L。

Study on purification and antibacterial activity of protamine from Takifugu obscurus

In this study,Sephadex G-50 and CM Sepharose Fast Flow were used to purify the spermatozoa of Takifugu obscui-us.Identification of protamine by arginine color reaction.The effects of protamine on the minimum inhibitory concentration,inhibition zone diameter and growth curve of Staphylococcus aureus,Escherichia coli and Salmonella were determined.The results showed that;The spermatozoa of Takifugu obscui-us had an absorption peak at 217 and 273 nm,respectively,and the absorption peak at 217 nm was higher.The purified protamine was found to be better than the protamine before purification by SDS-PAGE electrophoresis.The strips were fine and had no tailing.The Takifugu obscuj-us protamine had inhibitory effects on Gramnegative bacteria(Salmonella and Escherichia coli)and Gram-positive bacteria(Staphylococcus aureus),which could delay the bacteria growth,and the minimum inhibitory concentrations against Staphylococcus aureus,Escherichia coli and Salmonella were 500,1000 and 1000 mg/L,respectively.