金银花和蒲公英提取物对肉源性假单胞菌生物被膜的清除作用

为明确金银花和蒲公英提取物对假单胞菌生物被膜的清除效果,采用单因素实验确定假单胞菌生物被膜的最佳培养温度、培养时间和固定方法;在此条件下,采用结晶紫法结合扫描电镜研究金银花和蒲公英提取物在不同质量浓度、不同添加时间下对肉源性假单胞菌生物被膜的清除作用。结果表明,30 ℃培养24 h生物被膜测定值最高。提取物对生物被膜的清除效果随质量浓度的增加而逐渐增强(P<0.05),在生物被膜形成0 h加入的清除效果显著强于在24 h加入的清除效果(P<0.05)。当提取物浓度高于最小抑菌浓度(MIC)时,主要通过抑制菌体生长而清除生物被膜;当低于最小抑菌浓度时,主要通过破坏生物被膜立体结构而清除生物被膜。提取物破坏假单胞菌生物被膜结构,使生物被膜形成孔洞,从而清除生物被膜。本研究证明金银花和蒲公英提取物能够清除假单胞菌生物被膜,对肉的保鲜具有潜在应用价值。

Scavenging Effect of Honeysuckle and Dandelion Extracts on the Biofilm of Pseudomonas Stains from Meat

The scavenging effect of honeysuckle and dandelion extracts on the biofilm of Pseudomonas strains was researched in this study. The optimum culture temperature, time and fixing method of Pseudomonas were determined by single factor tests. The effects of honeysuckle extract and dandelion extract on the biofilm of Pseudomonas were evaluated by crystal violet method combined with scanning electron microscopy. The results showed that, Pseudomonas strains from meat had strong biofilm-forming ability with the highest value under 30℃ at 24 h. The scavenging effect of honeysuckle extract and dandelion extract on biofilm increased gradually with the increase of mass concentration (P<0.05). The honeysuckle extract and dandelion extracts, adding at 0 h had better scavenging effects on forming biofilm than adding at 24 h (P<0.05). The laser-scanning confocal microscope imaging results showed that, extracts scavenged biofilm by inbibiting bacterial growth above MIC by destroying the spatial structure of biofilm. With the extracts concentration was higher than MIC, the holes on mature biofilm were smaller and fewer. Extracts destroyed the structure of biofilm and lead to the formation of holes on the biofilm when lower than MIC. This study proved that honeysuckle and dandelion extracts had the effect of scavenging Pseudomonas biofilm with potential application value in meat preservation.