胭脂果多糖提取工艺优化及其抗氧化活性分析

为了考察超声辅助水提法对胭脂果多糖得率的影响,本研究应用单因素实验对超声功率、超声时间、提取温度和液料比展开了研究。在此基础上,采用响应面法优化了工艺参数,并分析了胭脂果粗多糖的体外抗氧化活性。结果表明,当胭脂果多糖最佳提取工艺为超声时间6 min、超声功率97 W、提取温度86℃、提取时间150 min和液料比40 mL/g时,粗多糖得率可达12.55%±0.31%,仅低于预测值0.23%,而且其中多糖含量达到了(413.75±0.41)mg/g,说明该模型能较好地预测实际得率。胭脂果多糖对DPPH·和·OH以及总还原能力与质量浓度呈量效关系,对DPPH·和·OH的IC₅₀分别为0.0203、1.44 mg/mL。因此,响应面法优化超声辅助水提法提取胭脂果多糖工艺方便可行,得到的多糖有较好的体外抗氧化活性,可为进一步的合理开发利用提供理论依据。

Optimization of Extraction Process of Polysaccharide from Padus napaulensis and Its Antioxidant Activity

In order to investigate the effect of ultrasound-assisted water extraction on the extraction rate of polysaccharides from Padus napaulensis,single factor experiments were carried out to study the ultrasonic power,ultrasonic time,extraction temperature and liquid-to-solid ratio. On this basis,the process parameters were optimized by response surface method,and the antioxidant activities of raw polysaccharides from Padus napaulensis in vitro were analyzed. The results showed that the extraction rate of raw polysaccharides could reach 12.55%±0.31% when the optimum extraction technology of polysaccharides was ultrasonic time of 6 min,ultrasonic power of 97 W,extraction temperature of 86℃,extraction time of 150 min and liquid-to-solid ratio of 40 mL/g,which was only less 0.23% than the predicted value. The contents of polysaccharides reached(413.75±0.41) mg/g,which indicated the model could predict the actual extraction rate well. The IC₅₀ of polysaccharide solution of Padus napaulensis for DPPH·,·OH and total reduction ability were 0.0203 and 1.44 mg/mL,respectively,and in which had dosage-effect relationship and good antioxidant activity in vitro. Therefore,it was feasible to optimize the extraction process of polysaccharides by ultrasound-assisted water extraction with response surface method,and could provide theoretical basis for further development and utilization of Padus napaulensis.