短梗大参多酚的提取及体外抗氧化性研究

为开发利用短梗大参树皮中的活性成分,本文对影响短梗大参多酚提取量的工艺参数(提取溶剂、乙醇体积分数、料液比、提取温度和提取时间)进行了优化;以DPPH自由基清除率和羟基自由基清除率为指标,研究了短梗大参多酚的体外抗氧化性能。结果表明:短梗大参多酚提取的最优工艺条件为乙醇体积分数50%、料液比1:40 (g/mL)、提取时间50 min、提取温度45 ℃,在该参数条件下多酚提取量为(0.320±0.02)mEq GA/g;短梗大参多酚对DPPH、羟自由基均具有较好的清除效果,其半清除浓度(EC₅₀)分别为43.4 μg/mL和37.6 μg/mL。短梗大参多酚具有较好的体外抗氧化活性,可作为一种潜在的抗氧化剂进行开发利用。

Optimization of Polyphenols Extraction from Macropanax rosthornii and Its Antioxidant Activity In Vitro

In order to exploit the active constituents of Macropanax rosthornii,this paper optimized the parameters(solvents,volume fraction of ethanol,the ratio of material to liquid,extraction temperature and extraction time)which influence the yield of the total polyphenol content. Meanwhile,the antioxidant activity of polyphenols in vitro was studied by using the DPPH and hydroxyl radical scavenging rate as indicators. The results showed that the optimum parameters for polyphenol extraction from Macropanax rosthornii were ethanol volume fraction of 50%,solid to liquid ratio of 1∶40 (g/mL),extraction time of 50 min and extraction temperature of 45 ℃,under which the extracted polyphenol content was(0.320±0.02) mEq GA/g. The results of the antioxidant activity showed that the polyphenols from Macropanax rosthornii presented good scavenging rate on DPPH and hydroxyl radicals,with its concentration of decrease 50% free radicals(EC₅₀)of 43.4 μg/mLand 37.6 μg/mL,respectively. Macropanax rosthornii indicates favourable antioxidant activity in vitro,and it deserves to exploit as a potential antioxidant.