| 双歧杆菌的分离与培养 |
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| 引用本文： | 秦玲玲,廖海印,金雨华,张宁,张鹭.双歧杆菌的分离与培养[J].齐齐哈尔轻工业学院学报,2008(3):78-82. |
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| 作者姓名： | 秦玲玲 廖海印 金雨华 张宁 张鹭 |
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| 作者单位： | 齐齐哈尔大学生命科学与工程学院,黑龙江齐齐哈尔161006 |
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| 摘 要： | 利用大多数双歧杆菌具有半乳糖苷酶且活性较高的特性，在改良的MRS培养基中加入X-Gal（5-溴-4-氯-3-吲哚-α-D-半乳糖苷）作为显色底物，产生颜色反应，进而从市售酸奶中分离出3株双歧杆菌，并对其中的1株进行生长曲线的绘制及培养基成分的优化，为今后双歧杆菌产品的开发及生物学功能的探索奠定基础。
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| 关 键 词： | 双歧杆菌 分离与鉴定 生长曲线 培养基 |
The separation and cultivation of Bifidobacterium |
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| Authors: | QIN Ling-ling LIAO Hai-yin JIN Yu-hua ZHANG Ning ZHANG Lu |
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| Affiliation: | ( College of Life Science and Engineer, Qiqihar University, Heilongjiang Qiqihar 161006, China ) |
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| Abstract: | Most Bifidobacterium have the galactose glueoside enzyme and higher enzyme activity, it can produce colour reaction in MRS ameliorating medium adding X-Gal （5-bromine-4-chlorines-3-benzazole-alpha-D-galactoses glucoside） as substrate. We separated three strains of Bifidobacterium in market yoghurt according to this characteristic. Then the growth curve was drew and the culture medium was optimized this study provide basic formation for yoghurt development and Bifidobacterium biological study. |
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| Keywords: | Bifidobacterium separation and identification growth curve culture medium |
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